Tissue-culturing rapid propagation method for dendrobium officinale

Abstract

The invention discloses a tissue-culturing rapid propagation method for dendrobium officinale. The tissue-culturing rapid propagation method includes the steps of primary culturing, wherein seed culturing is adopted, stem tip and caulicle culturing is carried out, and protocorm and lateral bud induction is carried out; secondary culturing, wherein repeated transferring culturing is carried out on buds or protocorm obtained through the primary culturing on culturing media comprising 1 / 2MS, 0.5 mg / L of 6_BA, 0.2 mg / L of NAA, 5% of mashed potatoes, 5% of mashed bananas and 2% of sugar; rooting and hardening-off culturing, wherein culturing media comprise 1 / 2MS, 0.2 mg / L of NAA, 0.2 mg / L of 6_BIBA, 5% of mashed potatoes, 10% of mashed bananas and 7.5% of sugar, and the pH value is 5.8; inoculation is carried out, and seedling hardening is carried out through media prepared in the mode that pure pine bark is adopted and soaked in 2,000-fold carbendazim liquid for 24 hours, and then piling fermentation is carried out for two months to three months. The seed germination rate is high, the bud-seedling growing speed is high, seedlings are thick, blade surfaces are unfolded, root systems are developed, and the survival rate is high; the aim of rapid and nontoxic propagation of dendrobium officinale seedlings is achieved.

Description

technical field [0001] The invention relates to a method for tissue culture and rapid propagation of dendrobium candidum. Background technique [0002] Dendrobium officinale (scientific name: Dendrobium officinale), also known as black knot grass, belongs to the epiphytic orchids of the aerial orchidaceae herb. Dendrobium can be divided into dozens of species such as yellow grass, gold hairpin, and horsewhip. It is named for its iron-green skin. Wild Dendrobium candidum only grows in the shady crevices of cliffs or on big trees in virgin forests, and has strict requirements on temperature, humidity, light and other microclimates. The plants only branch once a year, about one inch long, and at least 2 -3 years to harvest. It is a traditional precious Chinese herbal medicine. In "Compendium of Materia Medica Supplements", it is said that Dendrobium "clears the stomach, eliminates deficiency and heat, and produces body fluid. Dry polydipsia, lack of food and retching, defici...

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to disclose a method for tissue culture and rapid propagation of Dendrobium candidum, to solve the problem of Dendrobium candidum seedlings, and to provide basic conditions for the development of artificial planting of Dendrobium candidum