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Meta-zinc tetraphenylporphyrin-based electrogenerated chemiluminescence body as well as preparation method and application thereof

A tetraphenylporphyrin zinc, electrochemical technology, applied in the field of electrochemical analysis and detection, can solve the problems of application limitation of ruthenium bipyridine, complicated experimental equipment, high analysis cost, and achieve improved charge separation and transfer efficiency and high sensitivity. , the effect of excellent electrical conductivity

Active Publication Date: 2015-11-11
迈科若(苏州)医疗科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Taking ruthenium bipyridine as an example, it is expensive, and there are a series of problems such as high analysis cost, environmental pollution, and complicated experimental equipment caused by continuous consumption. Toxic and harmful to the environment, so that the application of ruthenium bipyridine is subject to certain restrictions

Method used

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  • Meta-zinc tetraphenylporphyrin-based electrogenerated chemiluminescence body as well as preparation method and application thereof
  • Meta-zinc tetraphenylporphyrin-based electrogenerated chemiluminescence body as well as preparation method and application thereof
  • Meta-zinc tetraphenylporphyrin-based electrogenerated chemiluminescence body as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] (1) Add 4.5 mg of footballene pyrrolidine tricarboxylic acid, 38.3 mg of N-ethyl-N'-(3-dimethylaminopropyl) carbodiimide, 5.8 mg of N- Hydroxysuccinimide and 4 mg aniline imidazole, magnetically stirred for 24 hours in isolation from air;

[0035] (2) After the reaction, transfer the above reaction product solution into a dialysis bag with a molecular weight cut-off of 1000, use DCM as the dialysate, change it every 2 to 3 hours, and dialyze at room temperature for 3 to 4 days to remove unreacted reactants ;

[0036] (3) Take the product solution after dialysis, add 17.0 mg m-tetraphenylporphyrin zinc, seal it away from light and magnetically stir for 2 hours, use a dialysis bag with a molecular weight cut-off of 2000 to purify the above reaction product, and the dialysis conditions are the same as the previous step;

[0037] (4) Obtain the reaction product solution after dialysis is finished, volatilize naturally and dry in vacuum to obtain with ImAn bridging C 60 an...

Embodiment 2

[0039] (1) Add 4.5 mg of football ene pyrrolidine tricarboxylic acid, 19.2 mg of N-ethyl-N'-(3-dimethylaminopropyl) carbodiimide, 5.8 mg of N- Hydroxysuccinimide and 2.4 mg aniline imidazole, magnetically stirred for 24 hours in isolation from air;

[0040] (2) After the reaction, transfer the above reaction product solution into a dialysis bag with a molecular weight cut-off of 1000, use DCM as the dialysate, change it every 2 to 3 hours, and dialyze at room temperature for 3 to 4 days to remove unreacted reactants ;

[0041] (3) Take the product solution after dialysis, add 10.2 mg m-tetraphenylporphyrin zinc, seal it away from light and magnetically stir for 2 hours, use a dialysis bag with a molecular weight cut-off of 2000 to purify the above reaction product, and the dialysis conditions are the same as the previous step;

[0042] (4) Obtain the reaction product solution after dialysis is finished, volatilize naturally and dry in vacuum to obtain with ImAn bridging C 60...

Embodiment 3

[0044] (1) Add 4.5 mg of footballene pyrrolidine tricarboxylic acid, 9.6 mg of N-ethyl-N'-(3-dimethylaminopropyl) carbodiimide, 5.8 mg of N- Hydroxysuccinimide and 4.7 mg aniline imidazole, magnetically stirred for 24 hours in isolation from air;

[0045] (2) After the reaction, transfer the above reaction product solution into a dialysis bag with a molecular weight cut-off of 1000, use DCM as the dialysate, change it every 2 to 3 hours, and dialyze at room temperature for 3 to 4 days to remove unreacted reactants ;

[0046] (3) Take the product solution after dialysis, add 20.3 mg m-tetraphenylporphyrin zinc, seal it away from light and magnetically stir for 2 hours, use a dialysis bag with a molecular weight cut-off of 2000 to purify the above reaction product, and the dialysis conditions are the same as the previous step;

[0047] (4) Obtain the reaction product solution after dialysis is finished, volatilize naturally and dry in vacuum to obtain with ImAn bridging C 60 a...

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Abstract

The invention discloses a meta-zinc tetraphenylporphyrin-based electrogenerated chemiluminescence body. The chemiluminescence body is prepared from aniline imidazole, footballene pyrrolidine tricarboxylic acid and meta-zinc tetraphenylporphyrin. The invention also provides a preparation method of the chemiluminescence body, comprising the step that on the commercial footballene pyrrolidine tricarboxylic acid, reacting with the meta-zinc tetraphenylporphyrin in an axial coordination way by taking the aniline imidazole as a bridging object, so as to generate the efficient and stable novel electrogenerated chemiluminescence body. On this basis, according to the envelope characteristics of beta-cyclodextrin molecules, a stable electrogenerated chemiluminescence biological sensing system with beta-cyclodextrin enveloping fixed chemiluminescence body molecules is designed and built, realizes high sensitivity and high specificity detection on DNA (deoxyribonucleic acid), and is stable in electrogenerated chemiluminescence output signal and lower in detection lower limit. The detection system can be applied to biological medicine, clinical diagnosis, environmental monitoring and the like.

Description

technical field [0001] The invention belongs to the technical field of electrochemical analysis and detection, and in particular relates to an electrochemiluminescent body based on m-tetraphenylporphyrin zinc, a preparation method and its application in DNA detection. Background technique [0002] Electrochemiluminescence (ECL) is known for its high sensitivity and low background. As a powerful analytical method, it has been applied in the food hygiene and medical industry. The sensitivity of ECL depends essentially on the luminous efficiency of the illuminant. Among them, cathodoluminescence is dominated by semiconductor nanocrystals, but such substances generally contain toxic components, and the ECL intensity is not high and relies on exogenous strong oxidants as co-reactants for sensitization, which greatly limits the further development of ECL technology. widely used. Therefore, it is very necessary to find highly efficient cathodic ECL luminophores and use them to de...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/30G01N27/26
Inventor 邓盛元季旭波孙文秀单丹
Owner 迈科若(苏州)医疗科技有限公司
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