A kind of cell volatile matter collection culture device

Abstract

The invention relates to a cell volatile matter collecting incubator for disease biomarker research. The cell volatile matter collecting incubator comprises a culture dish which is used for accommodating a to-be-detected liquid therein; a long elbow and a short elbow, wherein the inner ends of both the long elbow and the short elbow are arranged inside the culture dish; a beaker which is buckled in the liquid of the culture dish in an inverted mode, wherein the inner end of the long elbow is higher than the surface of the liquid and is located at the top of the beaker while the inner end of the short elbow is lower than the surface of the liquid and is located at the bottom of the beaker; and a crystallizing dish which covers and is buckled on the beaker, wherein the outer ends of both the long elbow and the short elbow extend out of the crystallizing dish. The incubator disclosed by the invention is a simple and non-sealed device, which not only can guarantee gas exchange between cells inside and outside the beaker but also can cause relatively low influence on cell activity, so that collected cell volatile matters are more significant in research; therefore, the incubator is more conducive to the exploration of disease metabolic mechanism and is beneficial for the preliminary screening and the early diagnosis of tumors and like diseases.

Description

technical field [0001] The invention relates to a cell culture device, in particular to a cell culture device for collecting volatile biomarkers of cells in a non-airtight environment. Background technique [0002] At present, the diagnosis of tumors mostly requires invasive examinations such as histopathology and biopsy, but these examinations take a long time, especially traumatic operations that bring inconvenience and pain to patients. For this reason, it is urgent to develop a simple, effective, economical, convenient and non-invasive diagnostic technique for early screening and clinical diagnosis of diseases. In recent years, people have studied the volatile organic compounds produced by solid tumors in various aspects, including blood, urine, exhaled breath, tumor tissue and tumor cells of tumor patients, trying to find volatile markers as early stage of the disease. New approaches to screening and diagnosis. The human body is a complex individual consisting of vari...

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Problems solved by technology

[0003] At present, there are literatures disclosing a cell culture and detection device for detecting volatile organic compounds in cells, which uses glass culture flasks for cultivation, but the device is airtight, and this airtight environment is not conducive to the gas exchange of cells. Failure to provide a suitable culture environment for cells can easily lead to hypoxic state of cells, resulting in cell apoptosis and affecting the normal metabolism of cells. In this environment, the organic compounds volatilized by cells are probably not produced by the cells under normal conditions. Substances are not conducive to the exact analysis of tumor markers, and it does not analyze the activity and state of cells in this culture environment, so the authenticity and reliability of the experimental results need to be further verified

[0004] Another document also discloses a cell culture device for detecting volatile organic compounds in cells, but the device has a complicated structure, and it needs to be directly connected with a gas tank containing 95% CO2 and 5% O2, and requires a catalyst, carbon Hydrogen compound trap, thermal distributed mass flow controller, sterile filter, water bath heating circuit, magnet, electronic control valve, dilution pipeline and other accessories, so the operation is relatively cumbersome and the cost is relatively high

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