Serum-free preservation liquid for umbilical cord mesenchymal stem cells and application of serum-free preservation liquid

Abstract

The invention provides serum-free preservation liquid for umbilical cord mesenchymal stem cells and application of the serum-free preservation liquid. The serum-free preservation liquid comprises normal saline with a NaCl mass fraction of 0.85-0.92%, insulin, estrogen, progestogen, hydrocortisone, sodium selenite, adenosine, N-acetylcysteine and L-glutamine. The insulin, the estrogen and the progestogen are added into the normal saline. Compared with serum preservation liquid, the serum-free preservation liquid and the application have the advantages that material sources capable of generating harm due to toxin with diversified components can be reduced to the greatest extent; the serum-free preservation liquid contains functional components with few components, basal metabolism of the cells can be maintained, and the cells are smooth in performance without huge variation; certain basic active nutrient substances and energy substances can be provided by the components, so that low metabolism level can be kept, the activity of the cells is in a stable state, shape variation or differentiation or the like due to growth and cultivation can be prevented, protection effects can be realized for the cells, the activity and the quality of the transplanted umbilical cord cells can be kept in a long-acting manner, and transplanted cell therapy clinical effects can be improved.

Description

technical field [0001] The invention belongs to the technical field of cell preservation solutions, and in particular relates to a serum-free preservation solution for umbilical cord mesenchymal stem cells and an application thereof. Background technique [0002] UC-MSCs (umbilical cord mesenchymal stem cells) have been widely used in cell transplantation replacement therapy, and the activity and quality of cells in transplantation and replacement therapy are the key to successful treatment. After the umbilical cord mesenchymal stem cells are cultured, UC-MSCs are preserved for a short period of time with the commonly used clinical cell preservation solution until the surgery and operation are suppressed. Among them, in the process of cell preservation, in order to ensure that the cells can continue to maintain cell metabolism and avoid other aspects of damage, etc., serum is added to the preservation solution as a cell protection component. [0003] After repeated research...

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Problems solved by technology

[0003] After repeated research and tracking, due to the existence of many unknown components in serum, including growth inhibitory factors, toxic factors, and other macromolecular proteins, etc.; although these components neutralize each other, the final cell properties change after preservation However, various substances such as intermediate growth inhibitory factors and toxic factors act on the cells to cause the stress response of the cells, which cannot be regarded as having never occurred, and these intermediate processes may include an increase in the level of oxidative stress, energy Metabolism will be unbalanced, ATP level will drop rapidly, etc., which will cause a significant decline in cell activity and quality, and eventually lead to failure to fuse with damaged tissue after transplantation and replacement therapy and death after a period of time, affecting the therapeutic effect

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