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A cell recovery explosion-proof egg

A cell and eggshell technology, which is applied in tissue cell/virus culture devices, biochemical instruments, biochemical equipment and methods, etc., can solve problems such as uneven heating of cell cryopreservation tubes, affecting cell recovery effect, and injury to experimenters , to achieve the effect of convenient use, uniform heating and guaranteed effect

Active Publication Date: 2017-07-04
CHINA TOBACCO YUNNAN IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide a cell recovery explosion-proof egg to solve the problem that the explosion of the cell cryopreservation tube during the cell recovery process will cause injury to the test personnel and the uneven heating of the cell cryopreservation tube will affect the cell recovery effect.

Method used

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  • A cell recovery explosion-proof egg
  • A cell recovery explosion-proof egg

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Embodiment 1

[0028] Such as figure 1 and figure 2 The shown cell recovery explosion-proof egg includes a lower half eggshell 1 and an upper half eggshell 2 that can be combined; wherein the bottom of the lower half eggshell 1 is a lead bottom 4, and the upper half of the eggshell 2 is It has 1 air hole 5 and 2 sampling tubes 3 . The lower half eggshell 1 and the upper half eggshell 2 are connected by a flexible connecting belt 6 , and the air hole 5 is located at the top of the upper half eggshell 2 . Wherein the sampling tube 3 has a certain length, and its purpose is to prevent water or other substances from spraying out from the sampling tube 3 when the cell cryopreservation tube 7 explodes.

[0029] The use process of the device is as follows: separate the lower half eggshell 1 and the upper half eggshell 2, pour 37-39°C warm water 8 into the lower half eggshell 1, and cover the upper half eggshell 2. Take the device to the liquid nitrogen tank where the cells are frozen, then take...

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Abstract

The invention relates to a cell thawing explosion-proof egg which comprises a lower half egg (1) and an upper half egg (2) which can be combined together, wherein the bottom of the lower half egg (1) is a lead bottom (4); air holes (5) and a sampling tube (3) are arranged on the upper half egg (2). The cell thawing explosion-proof egg is simple in structure, and convenient to use, and the problem that a tester can be injured due to explosion of a cell cryopreservation tube during a cell thawing process is solved; moreover, due to the design of the lead bottom, the cell thawing explosion-proof egg can wobble as a tumbler, so that the cell cryopreservation tube can be heated uniformly to ensure the cell thawing effect.

Description

technical field [0001] The invention belongs to the technical field of cell resuscitation devices, and in particular relates to a cell resuscitation explosion-proof egg. Background technique [0002] In cell-related experimental operations, cells are often cryopreserved and thawed. Cell recovery and culture is an important working basis in the biosafety evaluation system. The principle of cell recovery is rapid thawing. Cells frozen at -196°C in liquid nitrogen or other low-temperature environments must be quickly thawed to 37°C, so that the ice crystals in extracellular storage can be melted quickly, and the ice crystals that are slowly thawing can be prevented from entering the cells to form again. Crystallization will cause damage to the cells, especially at 0-5°C, the cells are more likely to be destroyed, so it is necessary to make the frozen cells pass through this temperature as soon as possible. [0003] The operation process of cell recovery is usually as follows:...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M3/00C12M1/38
CPCC12M41/12C12M45/20
Inventor 高茜向阳李雪梅曾婉俐夭建华黄海涛米其利
Owner CHINA TOBACCO YUNNAN IND