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bFGF(basic fibroblast growth factor)-optimized ADM (acelluar dermal matrix) barrier membrane for promoting sclerous tissue regeneration as well as preparation method and application of ADM barrier membrane

A barrier film and biological technology, applied in the field of biomedical engineering, can solve the problems of unobvious sustained release effect and weak adsorption capacity, and achieve the effects of promoting bone defect healing, strong adsorption capacity, and saving time

Inactive Publication Date: 2015-12-09
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Patent CN103656751A discloses a bFGF-ADM complex, which is applied to cover wounds, granulation wounds, chronic ulcers and decubitus sores to prevent water evaporation, protein and heat loss, prevent microbial infection, and promote wound healing. The method of electrostatic adsorption loads bFGF on the surface of ADM, the adsorption capacity is weak, and the sustained release effect is not obvious, and the complex is limited to soft tissue repair, and there is no report that it can be applied to bone tissue engineering

Method used

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  • bFGF(basic fibroblast growth factor)-optimized ADM (acelluar dermal matrix) barrier membrane for promoting sclerous tissue regeneration as well as preparation method and application of ADM barrier membrane
  • bFGF(basic fibroblast growth factor)-optimized ADM (acelluar dermal matrix) barrier membrane for promoting sclerous tissue regeneration as well as preparation method and application of ADM barrier membrane
  • bFGF(basic fibroblast growth factor)-optimized ADM (acelluar dermal matrix) barrier membrane for promoting sclerous tissue regeneration as well as preparation method and application of ADM barrier membrane

Examples

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Embodiment 1

[0040] Example 1 Preparation method of heparinized ADM membrane loaded with bFGF:

[0041] Raw materials: finished ADM film (Yantai Zhenghai Biotechnology Co., Ltd., type B Oral biofilm Haiao, please specify the company name and the model of the product you bought)

[0042] 1mmol / L heparin / heparin sulfate solution

[0043] 200ng / mL bFGF solution

[0044] (1) MES biological buffer configuration: Take 1L as an example, weigh 9.76gMES, 29.22gNaCl, 5mL10% Brij35, dissolve in 1L distilled water, adjust the pH to 6.0±0.1 with NaOH solution, and set aside; PBS buffer configuration: Take 1L as an example, weigh 9gNaCl, 6gNa 2 HPO 4 12H 2 O (or Na 2 HPO 4 2.38g), 0.4gNaH 2 PO 4 2H 2 O, dissolved in 1L distilled water, adjusted to pH 7.4 with NaOH solution, set aside.

[0045] The above buffers can also be purchased directly from reagent suppliers.

[0046] (2) Preparation of heparinized acellular dermal matrix membrane: place the finished ADM membrane in 0.1mol / L MES biolo...

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Abstract

The invention discloses a bFGF(Basic Fibroblast Growth Factor)-optimized ADM (acelluar dermal matrix) barrier membrane for promoting sclerous tissue regeneration as well as a preparation method and application of the ADM barrier membrane. A heparinized ADM membrane is soaked with a bFGF solution at 0-8 DEG C and then freeze-drying the membrane material at -60 DEG C to obtain a bFGF-loaded heparinized ADM membrane. According to the ADM barrier membrane as well as the preparation method and the application thereof, bFGF is loaded on the surface of a GTR (guided tissue regeneration) barrier membrane material, so that not only can a space retention function be developed, but also healing of skull defect of rats is accelerated through the actions of collecting mesenchymal stem cells and promoting proliferation. Therefore, according to the ADM barrier membrane as well as the preparation method and application thereof, an existing ADM membrane material is upgraded and the development of bone tissue engineering is promoted.

Description

technical field [0001] The invention belongs to the technical field of biomedical engineering, and specifically relates to a bFGF-optimized ADM barrier film for promoting hard tissue regeneration, a preparation method and application thereof. Background technique [0002] Various bone defects caused by congenital malformations, trauma and tumors have seriously plagued patients and medical workers. Bone loss caused by periodontitis has always been the main bottleneck hindering the development of periodontal medicine. In recent years, bone tissue engineering, as an emerging interdisciplinary subject, has brought new hope for the repair of bone defects. Among them, the barrier membrane of guided tissue regeneration (GTR) has the function of cell barrier and maintaining the space to guide specific tissue regeneration, so it has become an indispensable part of GTR. The ideal barrier membrane material should have good biocompatibility and bioabsorbability And appropriate mechanic...

Claims

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Application Information

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IPC IPC(8): A61L27/38A61L27/54
Inventor 杨丕山杜密
Owner SHANDONG UNIV
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