High-purity collagen sponge with biological activity and preparation method of high-purity collagen sponge

A collagen sponge and trypsin technology, applied in the field of medical biomaterials, can solve the problems of lack of high-purity, biologically active structure, etc.

Active Publication Date: 2015-12-23
MICONVEY TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In view of the shortcomings of the prior art described above, the object of the present invention is to provide a biologically active collagen sponge and its preparation method, which is used ...

Method used

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  • High-purity collagen sponge with biological activity and preparation method of high-purity collagen sponge
  • High-purity collagen sponge with biological activity and preparation method of high-purity collagen sponge
  • High-purity collagen sponge with biological activity and preparation method of high-purity collagen sponge

Examples

Experimental program
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Effect test

Embodiment 1

[0109] Fresh bovine Achilles tendons aged 18-24 months were taken, the fascia was removed, washed with purified water three times for 4 minutes each time, and then frozen in bundles in a low-temperature refrigerator at a temperature of -20 to -40°C. Freezing time ≥ 12h, and avoid repeated freezing and thawing. Cut the frozen Achilles tendon into 0.5mm slices with a microtome. Take by weighing 40g of bovine Achilles tendon thin slices, soak 3 times with 75% (V / V) ethanol aqueous solution of 1% (m / V) sodium bicarbonate as degreasing liquid, remove fat after soaking time is 20min, rinse 3 times with purified water , the time is 4 minutes until the pH is close to that of purified water, which is neutral.

[0110] Take the above treated Achilles tendon slices and disperse them in 2L of purified water, adjust the pH to 8.0, add trypsin with a final concentration of 0.1%, react at a reaction temperature of 37°C for 2 hours, rinse with purified water for 3 times for 4 minutes until t...

Embodiment 2

[0119] Take fresh Achilles tendon of 18-24 months old cattle, remove the fascia, rinse with purified water 5 times for 5 minutes, and then freeze them in bundles. The freezing temperature is -20 to -40°C, and the freezing time is for 12 hours, and avoid repeated freezing and thawing. Cut the frozen Achilles tendon bundle into 0.5mm thin slices with a microtome, weigh 40 g of the bovine Achilles tendon, soak it with 75% (V / V) ethanol aqueous solution of 1% (m / V) sodium bicarbonate as degreasing liquid 5 times, after soaking for 30 minutes to remove fat, rinse with purified water 5 times, each time for 5 minutes until the pH is close to that of purified water and becomes neutral.

[0120] Take the above treated Achilles tendon slices and disperse them in 1L of purified water, adjust the pH to 8.2, add trypsin with a final concentration of 0.1%, react at a reaction temperature of 40°C for 2 hours, rinse with purified water 5 times, each time for 5 minutes to reach the pH Close t...

Embodiment 3

[0129] Take fresh Achilles tendon of 18-24 months old cattle, remove the fascia, rinse with purified water 4 times for 3 minutes, and then freeze them in bundles. The freezing temperature is -20 to -40°C, and the freezing time is for 15 hours, and avoid repeated freezing and thawing. With slicer, frozen Achilles tendon bundle is cut into thin slices of 5 mm, and 40 g of bovine Achilles tendon is taken, soaked with 90% (V / V) ethanol aqueous solution of 5% (m / V) sodium bicarbonate as degreasing liquid for 2 After soaking for 15 minutes to remove fat, rinse with purified water for 4 times, each time for 10 minutes until the pH is close to that of purified water and becomes neutral.

[0130] Take the above treated Achilles tendon slices and disperse them in 400mL of purified water, adjust the pH to 8.5, add trypsin with a final concentration of 0.025%, react at a reaction temperature of 40°C for 2h, wash with purified water 4 times, each time for 10min to pH Close to purified wat...

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Abstract

The invention provides high-purity collagen sponge with biological activity and a preparation method of the high-purity collagen sponge. High-purity collagen with the purity higher than 99% is prepared through purification procedures of special enzymolysis, self-assembly, gel chromatography, dialysis and the like of achilles tendon of animals, and the collagen sponge is prepared through a special freeze drying procedure. According to the high-purity collagen sponge with biological activity and the preparation method of the high-purity collagen sponge, the prepared collagen sponge has a complete two-time self-assembly triple-helical structure, has a flat and fine surface and soft texture, can be used for natural cavity filling and rapid hemostasis of a wound, can be used for rapidly stopping bleeding to prevent hematopedesis of the wound and has low immunogenicity, and safety of the collagen sponge is better guaranteed.

Description

technical field [0001] The invention belongs to the technical field of medical biomaterials, and relates to a biologically active high-purity collagen sponge and a preparation method thereof, in particular to a biologically active high-purity collagen sponge in animal Achilles tendons and a preparation method thereof. Background technique [0002] Collagen is an extracellular protein, which is a fibrous protein twisted into a helical shape by three peptide chains. Collagen is the most abundant protein in the human body, accounting for more than 30% of the total protein in the body. Existing in almost all tissues, it is an extracellular matrix protein that exists in the form of insoluble fibers and has high tensile strength. It is very important for the formation of animal and human skin, blood vessels, bones, and cartilage. It is an important substance of connective tissue. Is the main factor to determine the toughness of connective tissue, collagen is also the most importan...

Claims

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Application Information

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IPC IPC(8): A61L15/32A61L15/42A61L15/60C07K14/78C08J9/28
Inventor 李康郑宗清
Owner MICONVEY TECH CO LTD
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