A liquid nitrogen grinding system

Abstract

The invention relates to the technical field of a liquid nitrogen grinding device used in physiology, biochemistry and molecular biology, in particular to a liquid nitrogen grinding system. It is characterized in that it includes a grinding pestle, the grinding pestle is provided with a grinding pestle frosted plastic cover, the mortar and the grinding pestle are correspondingly arranged in a low temperature tank, the mortar is provided with a mortar and grinding plastic cover, and the low temperature tank Correspondingly, a centrifuge tube suspension frame and a grinding pestle suspension frame are also provided. It can crush various test samples quickly, conveniently and effectively, especially for scientific research work with a large number of samples. It can not only save a lot of manpower and time, but also has the advantages of simplicity, speed, high efficiency and low cost, and will not Contamination between test samples. The system can be used at normal temperature, low temperature and ultra-low temperature, making the solution to the above problems more efficient, fast, economical and convenient.

Description

technical field [0001] The invention relates to the technical field of a liquid nitrogen grinding device used in physiology, biochemistry and molecular biology, in particular to a liquid nitrogen grinding system. Background technique [0002] In various physiological, biochemical or molecular biology researches in the fields of modern life science, food science and environmental science, it is often necessary to perform physical fragmentation pretreatment on test samples, such as RNA / DNA extraction, protein separation, mineral Nutrients (Na + 、K + 、PO 3 2- , Ca 2+ etc.) content analysis, soluble sugar content determination, soluble amino acid analysis, chlorophyll content determination and various metabolic enzyme activities (such as catalase, superoxide dismutase, glutathione reductase, etc.) analysis etc. These test samples include plants, animals or soil. The quality of sample crushing will directly affect the accuracy of subsequent test results. For example, in agi...

AI Technical Summary

Problems solved by technology

Its disadvantages are as follows: ① For the traditional mortar, when the number of test samples is large, grinding and transfer are time-consuming and inefficient; and the mortar needs to be carefully cleaned, dried, and sterilized before and after the test , and even high-temperature baking (for example, RNA extraction needs to be baked at 180°C for more than 6 hours), so it is laborious and time-consuming

In addition, such as extracting RNA or DNA, when ultra-low temperature grinding is repeatedly added with liquid nitrogen, the ground tissue powder will splash everywhere due to the strong liquid nitrogen boiling, and it is very easy to cause cross-contamination between samples due to insufficient operating experience. causing the test to fail

② For the special grinding mechanical equipment, its cost is expensive, not all laboratories or scientific research personnel can have enough funds to purchase, and for a very small number of samples, special mechanical grinding needs to consume more liquid nitrogen, which is economical not worth it

In the field of modern cutting-edge scientific research, each scientific research project contains many experimental steps, and the completion of each experimental step requires a lot of time and effort; It is unacceptable that the whole pre-experimentation wasted

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