Application of zoledronic acid in preparation of drugs for treating fatty liver diseases

A technology of fatty liver disease and zoledronic acid, which is applied in the field of zoledronic acid for the preparation of drugs for the treatment of fatty liver disease, can solve the problems that there is no zoledronic acid, etc., achieve low development, slow down steatosis, slow down The effect of lipid accumulation

Active Publication Date: 2016-01-06
NANJING UNIV
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AI Technical Summary

Benefits of technology

This patented method uses zebraleic acid (ZL) to make medicine that could help cure hepatic disorders such as alcohols syndrome or other causes associated with excessive consumption of certain substances like sugar. It also helps prevent abnormal buildup inside organs called lipids deposits which are harmful if they form too much. Zinc oxide was found effective against this problem but it had potential negative health consequences when taken into body due its high content of free radical oxygen atoms.

Problems solved by technology

This patented technical problem addressed in this patent relates to developing safe therapies for use against fatness associated conditions like NASH, DLB, LCH, etc., particularly when considering the potential benefits provided by zilefrigerators containing escalator(+)-5-oxycarboxilage hydroxyproline derivative).

Method used

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  • Application of zoledronic acid in preparation of drugs for treating fatty liver diseases
  • Application of zoledronic acid in preparation of drugs for treating fatty liver diseases
  • Application of zoledronic acid in preparation of drugs for treating fatty liver diseases

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preparation example Construction

[0034] Method 2 Preparation of Paraffin Sections

[0035] An appropriate amount of right lobe of liver tissue was taken, fixed with 4% paraformaldehyde for 24-48 hours, dehydrated with ethanol step by step, transparent in xylene, dipped in wax, embedded in paraffin, and sectioned with a thickness of 5 μm.

[0036] Method 3 HE staining of paraffin sections

[0037] The slices were soaked in xylene for 10min and 5min to dewax, and then hydrated in graded ethanol. After staining with hematoxylin for 4 minutes, wash with distilled water. with 0.25% NH 3 ·H 2After O bluish for 30s, wash with single distilled water. After dehydration with graded ethanol to 95% ethanol, stain with 0.5% eosin solution for 2-3 seconds, then dehydrate in 100% ethanol, and soak in xylene for 2 minutes. Finally, the slides were sealed with neutral gum.

[0038] Method 4 Preparation of Frozen Sections

[0039] An appropriate amount of tissue was taken from the same part of the right lobe of the live...

Embodiment 1

[0052] Example 1 Effect of zoledronic acid on food-induced non-alcoholic fatty liver

[0053] Clinically, fatty liver occurs in patients, and the liver is swollen in appearance, but the main reason is due to the accumulation of lipids. And this kind of lipid accumulation can be obtained by biopsy to obtain liver tissue, and the accumulation of lipid can be seen through HE staining and oil red staining, so direct detection of HE and oil red is a better way to observe lipid accumulation.

[0054] According to the aforementioned general materials and methods, firstly, zoledronic acid and placebo were administered to the mice in the high-fat diet group. In this embodiment, zoledronic acid is mainly administered through tail vein injection, and the main action site of the drug is the liver, and is injected once every two days. The dosage was 50 μg / kg zoledronic acid. Tissue triglyceride level detection and HE staining were performed on mice after administration, the experimental ...

Embodiment 2

[0061] Example 2 Effect of zoledronic acid on food-induced fatty liver injury

[0062] Similar to Example 1, according to the foregoing general materials and methods, at first high-fat-induced mice were administered with zoledronic acid and a placebo, and alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in mouse blood were detected after administration. ) level, the experimental data are shown in Table 3, and the results are as follows Figure 4 A and 4B are shown.

[0063] The levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in mouse blood after the administration of table 3

[0064]

[0065]

[0066] The HFD group was a high-fat diet group, the RC group was a normal diet group, PBS was a placebo, ZOL was zoledronic acid, and the dosage was 50 μg / kg.

[0067] It can be seen from Table 3 that for the HFD group, the levels of ALT and AST decreased significantly after administration of ZOL. Among them, in RC group, ALT and...

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Abstract

The invention provides application of zoledronic acid in preparation of drugs for treating fatty liver diseases. The fatty liver diseases include fatty liver diseases caused by food-borne and hereditary factors, especially non-alcoholic fatty liver diseases. According to the application, the zoledronic acid can be used for effectively treating the liver diseases and relieving liver lipid accumulation and fatty degeneration, has remarkable relief effect on the non-alcoholic fatty liver diseases and can reduce the levels of glutamic-pyruvic transaminase and glutamic oxalacetic transaminase in blood, so that a new way for treating the fatty liver diseases and liver function damage is open up, a new indication is provided for clinical application of the zoledronic acid, and the potential and promising application prospect is created for the zoledronic acid in liver disease treatment.

Description

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Claims

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Application Information

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Owner NANJING UNIV
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