Kit for differentiating and detecting normal plasma cells and clonal plasma cells and its application

Abstract

The invention discloses a kit for detection and identification of normal plasma cell and clonal plasma cell and application thereof. The kit is matched with a flow cytometry for usage. The kit is equipped with monoclonal antibody combination according to the following substances: Ig, kappa Light Chain, Ig, lambda Light Chain, CD19, CD117, CD138, CD56, CD38 and CD45. CD38 and CD138 are first used for the identification of the plasma cells; CD45, CD56, CD19 and CD117 are employed for recognizing normal or abnormal state of the plasma cell surface; gating is conducted on normal and abnormal phenotypes (R4 and R5); and then the expression ratio of cKappa and cLambda in normal and abnormal phenotypes are respectively observed. The kit of the invention is used to identify all cPCs and has the advantages of high accuracy, fast analysis and simple method.

Description

[0001] The invention belongs to the structural design of a special kit in the process of cell phenotype confirmation, and in particular relates to a kit for identifying clonal plasma cell phenotypes using an eight-color antibody combination and its application. Background technique [0002] Plasma cell disease refers to a group of diseases in which clonal plasma cells (clonal plasma cells, cPCs) proliferate excessively and produce a large amount of monoclonal immunoglobulin, and cPCs are the root cause of plasma cell disease. Immobilized immunoglobulin electrophoresis or detection of free light chains can help determine the presence, but not the proportion, of clonal plasma cells; cPCs are not diagnostic if they are non-secreting, ie, do not synthesize immunoglobulin. Traditional bone marrow morphology methods can identify the proportion of abnormally shaped or naive plasma cells, but cannot determine their clonality. In certain diseases, such as monoclonal gammopathy of undet...

AI Technical Summary

Problems solved by technology

At present, most of the literature reports use a combination of 3-4 color antibodies. Except for the gating antibody, only 1-2 other antibodies can be detected in each tube. If you want to detect multiple antibodies to determine whether the plasma cells are abnormal, you need to use the gating antibody repeatedly. , about 3-5 sets of antibody combinations are required, and a total of 9-20 antibodies are required; this leads to high costs, increased sample volume, time-consuming operation, and low sensitivity

Moreover, the 4-color antibody combination cannot include gating antibodies, membrane antibodies, and antibodies against cytoplasmic Ig light chains at the same time, and cannot meet the requirements of grouping gating and further analysis of clonality

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