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Microtube for blood type testing, integrated abo/rhd blood type testing card and its preparation

A detection card, an integrated technology, applied in the field of blood type detection, can solve problems such as low efficiency and cumbersome operation steps, and achieve the effect of improving efficiency and reducing operation steps

Active Publication Date: 2017-01-18
SHENZHEN DAKEWE BIOENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The current ABO / RhD blood typing test card needs to add diluted red blood cells to perform the test, and the operation steps are cumbersome and inefficient

Method used

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  • Microtube for blood type testing, integrated abo/rhd blood type testing card and its preparation
  • Microtube for blood type testing, integrated abo/rhd blood type testing card and its preparation
  • Microtube for blood type testing, integrated abo/rhd blood type testing card and its preparation

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preparation example Construction

[0040] The present invention also provides a method for preparing an integrated ABO / RhD blood typing test card, in a specific embodiment, comprising the following steps:

[0041] (1) prepare antibody diluent, the specific gravity of described antibody diluent is greater than serum separation gel and less than erythrocyte;

[0042] (2) Gel swelling: use physiological saline or the antibody diluent to swell the gel;

[0043] (3) Preparation of antibody working solution: anti-A monoclonal antibody, anti-B monoclonal antibody and anti-D monoclonal antibody were prepared by adding 7 volume parts of the antibody diluent for every 1 volume part of the antibody to prepare the antibody working fluid;

[0044](4) Preparation of specific gels: According to the ratio of adding 1.2-1.7ml of the antibody working solution per 1g of the swollen gel, they were respectively prepared into anti-monoclonal anti-A gels with IgM properties and anti-monoclonal anti-A gels with IgM properties. Anti-...

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Abstract

A micropipe (1) for blood grouping, a one-piece ABO / RhD blood typing test card, and a preparation method therefor. The micropipe (1) for blood grouping comprises a gel (11), an antibody working solution (12), and a serum separation gel (13) from bottom to top in order. The antibody working solution (12) comprises a blood group antibody and an antibody diluent that can make the blood group antibody remain active. The serum separation gel (13) has a specific gravity of 1.075-1.077, and is capable of thixotropy to turn into fluid from solid with the action of a centrifugal force of 150 G to 300 G. The specific gravity of the antibody diluent is greater than that of the serum separation gel (13) and less than that of the erythrocyte. Peripheral blood can be directly added to one-piece ABO / RhD blood typing test card prepared by using the micropipe for testing, and it is unnecessary to perform testing after extracting, by means of centrifugation, erythrocytes from the peripheral blood and diluting. Therefore, operation steps of the conventional ABO / RhD blood typing test card are reduced while the sensitivity and specificity of the blood type test card are guaranteed, and the efficiency is improved.

Description

technical field [0001] The invention relates to blood type detection, in particular to a microtube for blood type detection, an integrated ABO / RhD blood type detection card and a preparation method. Background technique [0002] Since the discovery of ABO blood type, ABO blood type has been widely used in clinical practice. Accurate typing of ABO blood type is very important and is the basis and guarantee for safe blood transfusion. The Rh blood group system is an important blood group system second only to the ABO blood group system, and it is also the most complex system in the red blood cell blood group system. The antigens in the Rh blood group system include D, C, E, etc., and the D antigen has the strongest antigenicity. If the red blood cell surface contains D antigen, it is RhD positive, if it does not contain D antigen, it is RhD negative. After RhD negative patients receive RhD positive blood, at least 20% of patients will develop an immune response to D antigen, w...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/80
CPCG01N33/80
Inventor 钟志荣周翔吴庆军
Owner SHENZHEN DAKEWE BIOENG
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