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Parallel plate flow chamber with ports and method of use thereof
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A flow chamber and port technology, applied in the methods of stress-stimulated microbial growth, chemical instruments and methods, biochemical equipment and methods, etc. activities, etc.
Active Publication Date: 2018-05-29
思安初有限公司
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None of these systems are currently commercially available for large-scale culture activities
There are a few companies on the market (e.g., Ibidi, Fluxion, Cellix, Cellasics, Integrated Biodiagnostics, and Glycotech) that sell some flow chambers based on a parallel plate design; however, most of them are based on small microfluidic channels and do not offer multiple flow channels. channel condition or readout method
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[0086] The following examples provide illustrative embodiments. Those skilled in the art, in light of this disclosure and one's ordinary level of skill in the art, will appreciate that the following examples are intended to be illustrative only and that many variations, modifications and changes may be employed without departing from the scope of the disclosed subject matter.
example 1
[0088] Biomarker Analysis of Fluid Flow Regulated Cells
[0089] Differences in gene expression under two different shear stress conditions were tested in human aortic endothelial cells using a flow cell setup.
[0090] Table 1 shows the number of genes changed between human aortic endothelial cells exposed to a wall shear stress of 1.0 Pa for 20 hours compared to cells not exposed to flow. Cells were grown on collagen I-coated growth surfaces under static conditions (no flow) until confluence was reached. Cells were then exposed to fluid flow in the flow chamber at 0.2 or 1.0 Pa for 20 hours, or maintained in static culture for the same amount of time. After 20 hours, use Ambion MIRVANA TM The RNA Isolation Kit (Life Technologies, Foster City, CA, USA) isolated RNA from the cells and performed the analysis on Affymetrix PRIVIEW TM Array (Affymetrix Corporation, Santa Clara, California, USA) for analysis. Three experiments were performed for each condition, repeating th...
example 2
[0096] Drug treatment of fluid flow preconditioned and statically cultured cells
[0097] PI103(3-[4-(4-morpholino)pyrido[3',2',4,5]furo[3,2-d] Pyrimidin-2-yl]phenol; CAS No. 371935-74-9), a flow cell setup was also used to test for gene expression differences in human aortic endothelial cells under different shear stress conditions.
[0098] Cells were grown on collagen I-coated growth surfaces and treated with 100 nM PI-103 under flow. For the 4 hour time point, cells were exposed to fluid flow for 16 hours and treated with PI-103 for the final 4 hours with flow. Cells were also treated with PI-103 throughout the duration of flow (20 hours). The data were generated using the extracted RNA as described in Example 1 and presented in Affymetrix PRIVIEW TM Array (Affymetrix) was used for analysis. Three experiments were performed for each condition, repeating the microarray analysis.
[0099] Table 2 shows the number of genes that were significantly different between pair...
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Abstract
A flow chamber is provided. In some embodiments, the flow chamber includes: an inner plate having at least one flow channel formed therein, the at least one flow channel having a port on each end, wherein the port is adapted to releasably receive the septum; one or a plurality of ports adapted to releasably receive the plug and in at least fluid communication with the at least one flow channel; and an outer frame defining an exterior of the at least one flow channel and defining a perimeter of the flow chamber. In some embodiments, the flow chamber has the overall dimensions of a standard multiwell plate, and the at least one flow channel is located at a position corresponding to the column position of the standard multiwell plate. Also provided are methods for fabricating the flow chambers of the present disclosure and using the flow chambers to examine biological characteristics of cultured cells and / or tissues.
Description
[0001] Cross-references to related applications [0002] The subject matter of the present disclosure claims priority from US Provisional Patent Application Serial No. 61 / 791,770, filed March 15, 2013, the entire contents of which are hereby incorporated by reference. technical field [0003] The subject matter of the present disclosure relates generally to parallel plate flow cells and methods of using the same to examine the effects of different fluid flows on cells and their biological activity. In particular, the disclosed subject matter relates to devices on which cells and / or tissues can be cultured and examined in response to different fluid flow environments. Background technique [0004] In vitro cell culture is often performed as part of a variety of biological research and R&D projects. In its most common form, cell-based experiments are performed in petri dishes or flasks under static conditions (ie, those conditions in which no external force is applied to the ...
Claims
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