A kind of preparation method and application of bacillus cereus monoclonal antibody

Abstract

The invention provides a preparation method and application of a bacillus cereus monoclonal antibody. The preparation method comprises the following steps of: 1, preparing bacterial antigen through fixation of bacillus cereus by incubation treatment of paraform; 2, injecting the bacterial antigen so as to avoid emulsification; 3, performing short spacing impulse injection so as to quickly promote immunization; 4, planting solid tumors so as to rejuvenate tumor cells; 5, performing chemical induction and fusion; 6, performing semifluid selection so as to obtain monoclonal hybridoma; 7, performing cooperation of negative screening and positive screening so as to obtain a specific antibody; and 8, using a polyclonal antibody as a capture antibody and using a monoclonal antibody as a detection antibody to establish dual-antibody sandwich ELISA.

Description

technical field

[0001] The invention belongs to the field of biotechnology, in particular to a preparation method and application of a Bacillus cereus monoclonal antibody. Background technique

[0002] Food poisoning is a food-borne disease that seriously endangers human health. Most food poisoning is caused by harmful bacteria contaminating food. In bacterial food poisoning, Bacillus cereus food poisoning is more common. Bacillus cereus is an aerobic and facultative anaerobic Gram-positive bacillus widely found in a variety of plant foods and raw and cooked foods. Bacillus cereus is an opportunistic pathogenic bacterium that can produce diarrhea toxin and vomitoxin to cause food poisoning. Sometimes it can also cause human eye diseases, endocarditis, meningitis and other diseases through bacterial infection. As early as 1906, the first Bacillus cereus food poisoning was reported, and then many northern and eastern European countries also reported similar poisonings. Accor...

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