A kind of preparation method and application of bacillus cereus monoclonal antibody
A Bacillus cereus and monoclonal antibody technology, applied in the biological field, can solve the problems of time-consuming, poor sensitivity, and many influencing factors, and achieve the effects of ensuring animal welfare, good specificity, and promoting animal immunity
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[0033] The present invention is further described in detail by the following examples.
[0034] Preparation of Whole Cell Antigen of Bacillus cereus
[0035] Resuscitation and activation of Bacillus cereus in this laboratory. Prepare LB medium (weigh 10 g of tryptone, 5 g of yeast yeast powder and 10 g of NaCl per liter, pH 7.0), and autoclave at 121° C. for 20 minutes. Under sterile environment conditions, add 1mL of Bacillus cereus preserved at ~80°C to 25mL of liquid medium, and culture in an incubator at 37°C and 220rpm for 15h. Centrifuge at 10,000 rpm for 25 minutes and discard the supernatant to obtain bacterial pellets. Prepare sterile physiological saline, weigh 0.9g NaCl and dissolve in 100mL distilled water, and autoclave at 121°C for 20 minutes. Resuspend the above bacterial pellet with sterile normal saline, centrifuge at 10,000rpm for 20min, pour off the supernatant, wash 3 times in reciprocating cycles, and resuspend wit...
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