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Goat anti-rabbit retinoic acid binding protein 5 polyclonal antibody, and preparation method and application thereof

A technology of polyclonal antibody and binding protein, applied in the field of goat anti-rabbit retinoic acid binding protein 5 polyclonal antibody and its preparation

Inactive Publication Date: 2016-01-13
SHANXI AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Retinoic acid binding protein 5 is a secreted small molecular protein highly expressed in goat epididymis head. Specific antibodies are needed to study the expression localization characteristics and biological functions of the protein. and goat retinoic acid binding protein 5 polyclonal antibody

Method used

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  • Goat anti-rabbit retinoic acid binding protein 5 polyclonal antibody, and preparation method and application thereof

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preparation example Construction

[0016] The present invention also provides a preparation method of the above-mentioned goat anti-rabbit retinoic acid binding protein 5 polyclonal antibody, comprising the following steps:

[0017] S1. Using 18 amino acids at the N-terminal 81-98 of the retinoic acid binding protein 5 protein as the epitope of the goat anti-rabbit retinoic acid binding protein 5 polyclonal antibody, the sheep retinoic acid binding protein 5 polypeptide was synthesized with a purity of >75 %;

[0018] S2. Take 1.5 mg of the sheep retinoic acid binding protein 5 polypeptide obtained in step S1 and couple it to KLH, and use it as an immunogen after desalting;

[0019] S3, using the AbMax standard procedure (42 days), using the immunogen obtained in step S2 to carry out several booster immunizations on rabbits immunized with the immunogen KLH-CMKEKNQAVKGDAPGKFK polypeptide, and the multi-antibody titer of the polypeptide-BSA reaches the standard (serum titer ≥ 1:50000 ), bled 20ml of each animal ...

Embodiment 1

[0027] For immunofluorescence chemistry of goat epididymal head tissue, the antibody dilution ratio is 1:50. The green fluorescent area is the strong positive signal area of ​​retinoic acid binding protein 5.

Embodiment 2

[0029] For goat epididymis immunohistochemistry, the antibody dilution ratio is 1:50. The brown area is the strong positive signal area of ​​retinoic acid binding protein 5.

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Abstract

The invention discloses a goat anti-rabbit retinoic acid binding protein 5 polyclonal antibody, and a preparation method and an application thereof. The antibody can be used for goat and sheep immunohistochemistry, immunofluorescence chemistry and Western Blotting detection, wherein the concentration ratio is 1:50-200 when the antibody is used in the immunohistochemistry; the concentration ratio is 1:50-200 when the antibody is used in the immunofluorescence chemistry; and the concentration ratio is 1:500-1000 when the antibody is used in Western Blotting.

Description

technical field [0001] The invention relates to the field of biology, in particular to a goat anti-rabbit retinoic acid binding protein 5 polyclonal antibody and its preparation method and application. Background technique [0002] Retinoic acid binding protein 5 is a secreted small molecular protein highly expressed in goat epididymis head. Specific antibodies are needed to study the expression localization characteristics and biological functions of the protein. and goat polyclonal antibody to retinoic acid binding protein 5. Contents of the invention [0003] In order to solve the above problems, the present invention provides a goat anti-rabbit retinoic acid binding protein 5 polyclonal antibody and its preparation method and application. [0004] In order to achieve the above object, the technical scheme that the present invention takes is: [0005] Goat anti-rabbit retinoic acid binding protein 5 polyclonal antibody, its nucleotide sequence is [0006] TGCATGAAGGA...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K16/18C07K16/06
Inventor 张春香任有蛇郭丽娜刘文忠白元生师周戈赵宇琼焦光月
Owner SHANXI AGRI UNIV
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