Purification and rejuvenation culture method of ganoderma lucidum strain and culture medium thereof

Abstract

The invention belongs to the field of fungus cultivation and provides a lucid ganoderma fungus purification and rejuvenation cultural method and a culture medium thereof against the problem that the activity of lucid ganoderma fungi is lowered due to an existing traditional lucid ganoderma fungus culture medium. The cultural method comprises the following steps: 1, preparing the lucid ganoderma fungus rejuvenation culture medium, wherein the lucid ganoderma fungus rejuvenation culture medium is prepared from 150-400 g of potatoes, 10-30 g of glucose, 10-25 g of agar powder, 1-5 g of monopotassium phosphate, 0.5-3 g of magnesium sulfate and 30-70 g of lucid ganoderma sporocarp; 2, culturing lucid ganoderma fungi into hypha, and then inoculating the hypha to the lucid ganoderma fungus rejuvenation culture medium obtained in step 1; 3, conducting culturing according to normal conditions, and obtaining rejuvenation fungi. The hypha obtained through the cultural method is quick in growth, pure white, orderly and dense, and the obtained sporocarp is good in agronomic trait.

Description

technical field [0001] The invention belongs to the field of fungal cultivation, and in particular relates to a method for purifying and rejuvenating ganoderma fungi and a culture medium thereof. Background technique [0002] Ganoderma lucidum is a treasure in the treasure house of Chinese medicinal materials. It is known as the "immortal grass" and is the source of Chinese medicine. As a clinical raw material of traditional Chinese medicine, Ganoderma lucidum has a history of more than 3,000 years. Ganoderma lucidum has been artificially cultivated in my country for more than 30 years. The cultivation area is wide and there are many varieties. The quality of Ganoderma lucidum directly affects the quality and curative effect of Chinese medicine products. Modern pharmacological research and clinical efficacy have proved that the medicinal value of Ganoderma lucidum includes anti-tumor and anti-radiation functions, anti-cardiovascular system diseases, improving immunity, prote...

AI Technical Summary

Problems solved by technology

[0004] Aiming at problems such as the reduction of the activity of the ganoderma strains caused by the existing traditional ganoderma strain culture substrate, the purpose of the present invention is to provide a new rejuvenation medium for the ganoderma lucidum strains and a culture method thereof, which can solve the large-scale problem of ganoderma lucidum strains. Problems such as reduced stress resistance, insufficient activity, poor vitality, and low yield of strains in production or even industrialized cultivation

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