Method for simultaneously quantitatively determining content of multiple phenols compositions in gastrodia elata Bl.

A technology for the quantitative determination of gastrodia, which is applied in the field of detection of the traditional Chinese medicine Gastrodia elata, can solve the problem of low sensitivity of the method, and achieve the effect of high sensitivity, good precision and clear components

Inactive Publication Date: 2016-01-27
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current literature or patent methods can only realize the determination of 1 to 4 phenolic components or total gastrodin in Gastrodia elata (Liu Yuhong, Journal of Pharmaceutical Analysis, 2010, 30 (1): 30-32) (Liu Yuhong, Zhong Patent Medicine, 2012, 34(1): 182-184) (Liu Zhi, Chinese Journal of Pharmaceutical Sciences, 2012, 40(5): 380-383), it is difficult to objectively and truly reflect all the active ingredients of Gastrodia elata exerting medicinal effects in vivo, And the sensitivity of the method is low
In addition, there is no report on the simultaneous determination of multiple phenolic components in Gastrodia elata

Method used

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  • Method for simultaneously quantitatively determining content of multiple phenols compositions in gastrodia elata Bl.
  • Method for simultaneously quantitatively determining content of multiple phenols compositions in gastrodia elata Bl.
  • Method for simultaneously quantitatively determining content of multiple phenols compositions in gastrodia elata Bl.

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preparation example Construction

[0030] The preparation method of Gastrodia elata extract is a solvent extraction method, wherein preferred ethanol-water extraction, the extraction method can be any one of reflux extraction, ultrasonic extraction, microwave-assisted extraction, the preferred preparation method is: Gastrodia elata medicinal material with 5- 10 times the volume concentration of 70% ethanol-water reflux extraction for 2 to 3 times, the extracts were combined and concentrated, dispersed with an appropriate amount of water, extracted 2-4 times with 1-2 times the volume of ethyl acetate, and the obtained water layers were combined and concentrated, that is have to.

[0031] Methanol (chromatographically pure), water is deionized water, and the rest are analytically pure.

Embodiment 1

[0032] Embodiment 1 The optimization of excitation wavelength, emission wavelength and chromatographic conditions

[0033] Screening for excitation-emission full-wavelength scans

[0034] Take appropriate amounts of gastrodin, p-hydroxybenzyl alcohol, parishinB, parishinC and parishin reference substances, weigh them accurately, dissolve and dilute them with 8% methanol-water to form a reference substance stock solution with a concentration of 1 mg / ml.

[0035] Precisely draw a certain amount of 5 kinds of reference substance stock solutions, add 8% methanol-water to dilute into a mixed reference substance solution with a concentration of 100ng / ml for condition optimization analysis;

[0036] The mixed solution of gastrodin, p-hydroxybenzyl alcohol, parishinB, parishinC and parishin reference substance with a concentration of 100ng / ml was scanned at the full excitation wavelength (200-290nm), and it was found that these five components had a maximum absorption at 225nm, as figu...

Embodiment 2

[0039] The content detection of gastrodin, p-hydroxybenzyl alcohol, parishinB, parishinC and parishin in the gastrodia elata extract of embodiment 2

[0040] Chromatographic conditions

[0041] The chromatographic column is Agilent ZORBAXSB-C18 (3.0×100mm, 1.8μm), the mobile phase is methanol (A)-0.5% formic acid solution (B), the elution program: 0-5min, A increases linearly from 8% to 40%, 5 -12min, A maintained at 40%; column temperature: 50°C; flow rate: 0.35ml / min; excitation wavelength 225nm, emission wavelength 310nm; injection volume 10μl. Get the test solution, sample injection analysis under the above-mentioned chromatographic conditions, the resolution of each component to be tested in the sample and the adjacent peak is greater than 1, and the chromatogram is as follows: Image 6 shown.

[0042] Preparation of reference solution

[0043] Take appropriate amounts of gastrodin, p-hydroxybenzyl alcohol, parishinB, parishinC and parishin reference substances, weigh ...

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Abstract

The invention relates to a content determining new method for simultaneously determining content of gastrodin, p-hydroxybenzylalcohol, parishin B, parishin C, parishin and other phenols compositions in gastrodia elata Bl.. The main steps comprise performing separation by utilizing rapid high performance liquid chromatography employing methanol or acetonitrile and an acid water with the volume percentage of 0.1%-0.5% as a mobile phase, determining the content of the target compounds by utilizing a high-sensitivity fluorescence detection process, and controlling the excitation wavelength to be 215-235 nm and the emission wavelength to be 295-335 nm. The method is high in sensitivity, good in precision and short in analysis time, and is applicable to rapid sensitive determination on phenols compositions in gastrodia elata Bl. medicinal material and extracts.

Description

technical field [0001] The invention relates to the detection field of traditional Chinese medicinal materials, in particular to a detection method of the traditional Chinese medical medicinal gastrodia elata. Background technique [0002] Gastrodia elata, also known as Chijian, Dingfengcao, and Duyaozhi, is the dry tuber of Gastrodia elata Bl. in the family Orchidaceae. The "Shen Nong's Materia Medica" listed it as a top grade two thousand years ago. It has the effects of calming the liver and calming the wind, dredging the collaterals and relieving pain, and is known as the "magic medicine for treating wind". Indications headache dizziness, limb numbness, epilepsy convulsion embolism. Gastrodin is the main active ingredient of Gastrodia elata. In the 2010 edition of the Chinese Pharmacopoeia, the content of gastrodin is used to control the quality of Gastrodia elata medicinal materials. The method for determining its content is high performance liquid chromatography, usin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88
Inventor 肖红斌唐春兰王莉
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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