Nucleic acid amplification reaction apparatus

Abstract

The invention provides a nucleic acid amplification reaction apparatus which can more stably amplify nucleic acid in a method for moving the nucleic acid amplification reaction solution in the oil with different temperature zones and performing the nucleic acid amplification reaction at a high speed. The nucleic acid amplification reaction apparatus includes a first heating section and a second heating section configured to heat a first region and a second region of a nucleic acid amplification reaction container respectively to a first temperature and a second temperature and a driving mechanism configured to switch the arrangement of the first region and the second region in the order of a first arrangement, a second arrangement, and a third arrangement. The second temperature is higher than the first temperature. The first arrangement and the third arrangement are arrangements in which the second region is below the first region in a direction in which the gravity acts. The second arrangement is an arrangement in which the first region is below the second region in the direction in which the gravity acts. The nucleic acid amplification reaction container includes a projecting section where an inner wall of the nucleic acid amplification reaction container projects outward. The projecting section is configured to enable the reaction liquid to stay in the projecting section in the third arrangement.

Description

technical field [0001] The invention relates to a nucleic acid amplification reaction device. Background technique [0002] As a method for amplifying nucleic acid at a high speed, a method is known in which oil and a small amount of nucleic acid amplification reaction solution are put into a cylindrical nucleic acid amplification reaction container, one end of the container is maintained at a high temperature, and the other end is maintained at a high temperature. Low temperature, rotate the container, and alternately switch to the state where the reaction solution is placed in the high-temperature oil in such a way that one end of the nucleic acid amplification reaction container is vertically downward, and the other end of the nucleic acid amplification reaction container is vertical. In the downward direction, the reaction liquid is placed in a low-temperature oil state, and thermal cycles are generated at high speed according to the temperature of the nucleic acid ampli...

AI Technical Summary

Problems solved by technology

The result may be biased in the amount of nucleic acid amplification

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