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Molecular markers linked to ZYMV (zucchini yellow mosaic virus-1) dominant disease-resistant gene ZYMV-1 and application of molecular markers

A technology of ZYMV-1 and molecular markers, applied in the field of molecular biology, can solve the problems of long cycle, few disease-resistant varieties, and large influence of human operation factors, and achieve the effects of convenient detection, stable amplification, and convenient identification

Inactive Publication Date: 2015-04-29
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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Problems solved by technology

However, at present, disease resistance genes are mainly derived from wild pumpkin materials, and there are difficulties in the transfer of resistance traits, and the linkage of unfavorable traits is cumbersome. Traditional disease resistance inoculation identification methods are greatly affected by environmental and human factors, and the accuracy and stability of identification results are not high. , leading to low efficiency of disease-resistant breeding, long cycle, and few disease-resistant varieties, which are far from meeting production needs
However, there is no research report on molecular markers closely linked to zucchini ZYMV disease resistance gene

Method used

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  • Molecular markers linked to ZYMV (zucchini yellow mosaic virus-1) dominant disease-resistant gene ZYMV-1 and application of molecular markers
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  • Molecular markers linked to ZYMV (zucchini yellow mosaic virus-1) dominant disease-resistant gene ZYMV-1 and application of molecular markers

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] (1) Creation and phenotypic identification of inbred lines BS3 and BS12F2 of zucchini:

[0037] (1) The zucchini inbred line BS3 (the female parent) and BS12 (the male parent) are crossed to obtain the hybrid F1, and the F1 self-crossing produces the F2 generation population;

[0038] (2) The single plant of the F2 generation colony was planted in a greenhouse nutrition pot, covered with an insect-proof net, and the cotyledons were fully expanded and inoculated with a virus disease. After 15 days of inoculation, a disease resistance trait was investigated. The identification results are shown in Table 1.

[0039] Table 1 Genetic analysis of BS3×BS12F2 generation after inoculation with ZYMV virus disease

[0040]

[0041] R and S represent resistant and susceptible individual plants, respectively. x20.05, 1=3.84;

[0042] (3) After the individual plant of the F2 generation was investigated for the traits, the susceptible plants were transplanted in the greenhouse. T...

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Abstract

The invention relates to molecular markers linked to a ZYMV (zucchini yellow mosaic virus) dominant disease-resistant gene ZYMV-1 and application of the molecular markers. The genetic distances between the molecular markers ZY-138 and ZY-157 and the disease-resistant gene are 0.4 cM and 2.6 cM respectively. The molecular markers ZY-138 and ZY-157 are subjected to PCR amplification with primers; and whether a resistance character transformed progeny plant has the disease-resistant gene ZYMV-1 or not can be accurately detected through the products of amplification. The molecular markers have the benefits as follows: the efficiency of breeding for disease resistance is remarkably improved, the cycle of breeding is provided, and a guarantee is provided for selection of ZYMV disease-resistant varieties; and the molecular markers ZY-138 and ZY-157 have the advantages of reliability, stability, simplicity in operation, high repeatability and the like and achieve very high utilization values in distinguishing resistant / susceptible varieties and resistant individual plants.

Description

technical field [0001] The invention belongs to the field of molecular biology, and particularly relates to a linked molecular marker of ZYMV dominant disease resistance gene ZYMV-1 in zucchini and its application. Background technique [0002] Zucchini is one of the most important vegetables planted in the world. The cultivation area in my country is increasing year by year. Especially in recent years, the facility cultivation in protected areas has developed rapidly, and it has become the second largest cultivated crop after cucumber in protected areas. It is of great significance to the increase of farmers' income, the adjustment of agricultural structure and the enrichment of the people's vegetable basket. [0003] Zucchini yellow mosaic virus (ZYMV) is one of the main diseases that endanger the production of cucurbit crops such as zucchini. ZYMV spreads rapidly around the world, and in recent years, the harm of zucchini production in my country has become increasingly ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/11C12N15/10C12Q1/68
Inventor 李海真
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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