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Plant online glucose detection method and device based on microelectrode biosensing technology

A microelectrode and glucose technology, applied in the field of electrochemistry, can solve the problems of complex sample processing, high extraction purity requirements, inability to perform dynamic analysis, long-term tracking and monitoring, etc., and achieve the effect of simple detection conditions

Active Publication Date: 2017-10-31
BEIJING RES CENT FOR INFORMATION TECH & AGRI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This type of method needs to sample the plant material in vitro, and calculate the content in the plant by detecting the concentration of glucose in the extract, which requires high purity of the sample extraction. Momentary concentration cannot be used for dynamic analysis and long-term follow-up monitoring
[0004] In order to realize the research on plant growth and environmental adaptability, it is necessary to carry out in-situ dynamic detection in vivo. Currently, the glucose in vivo detection products on the market are mainly aimed at animals or human bodies. Because plants have thin leaves, less tissue fluid, and lignified stems, etc. The above-mentioned detection products cannot be directly applied to plants, so it is urgent to develop an online glucose detection method and device for plant tissues

Method used

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  • Plant online glucose detection method and device based on microelectrode biosensing technology
  • Plant online glucose detection method and device based on microelectrode biosensing technology
  • Plant online glucose detection method and device based on microelectrode biosensing technology

Examples

Experimental program
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Effect test

Embodiment 1

[0027] The preparation of embodiment 1 glucose oxidase microelectrode

[0028] The glucose oxidase microelectrode prepared in this example is 5 cm long, and its tip part is 10 mm long; the base electrode material of the microelectrode is a silicon wafer, and the microelectrode has a three-electrode system, including Ag / AgCl reference electrode, platinum Counter electrode and gold working electrode modified by glucose oxidase. ( figure 1 )

[0029] Among them, gold working electrode 2, Ag / AgCl reference electrode 3, and platinum counter electrode 4 are sequentially arranged on the tip of silicon chip substrate 1; the above three electrodes.

[0030] The length of the silicon wafer base is 5 cm, the thickness is 1.5 mm, and the length of the tip part is 4 mm; the exposed tip part of the silicon wafer base 1 is provided with a gold working electrode 2, an Ag / AgCl reference electrode 3, and a platinum counter electrode 4 in sequence. ; The other end of the silicon wafer base i...

Embodiment 2

[0042] Example 2 Real-time online detection of glucose content in potato stem samples based on microelectrode biosensing technology

[0043] After the glucose oxidase microelectrode prepared in Example 1 was cleaned with deionized water, three parts of standard concentration (0.1mmol / L, 1mmol / L, 5mmol / L) glucose solutions were detected respectively, and electrochemical calibration was carried out to obtain the glucose concentration After plotting the relationship with the response peak current, obtain the slope. When the deviation between the slope and the slope of the working curve is within 15%, it is considered that the enzyme activity is normal and the electrode can work normally.

[0044] Insert the cleaned and calibrated microelectrode into the potato stem tissue to be tested, and connect it to the electrochemical workstation for cyclic voltammetry scanning for 5 minutes to obtain a stable cyclic voltammetry curve. The obtained response peak current value is substituted i...

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Abstract

The invention provides an online glucose detection method for plants based on microelectrode biosensing technology. The glucose oxidase microelectrode is inserted into the plant tissue to be tested, connected to an electrochemical workstation for cyclic voltammetry scanning, and the online glucose detection of the plant tissue is realized. . The present invention also provides a device for realizing the above detection method. The device includes an electrochemical workstation and a glucose oxidase microelectrode, and the two are connected by wires. The invention can realize the real-time detection of the glucose content of the plant, and the detected part of the plant does not need to be isolated from the body, without destructive damage, and only needs to clean the surface and fix the tissue to detect in vivo, and obtain continuous results for dynamic analysis. The detection device of the invention is portable, has simple detection conditions, can be operated in the field, and realizes continuous on-line detection.

Description

technical field [0001] The invention relates to the field of electrochemistry, in particular to a plant online glucose detection method and device based on microelectrode biosensing technology. Background technique [0002] Glucose is a monosaccharide, a hexose containing aldehyde groups. It widely exists in plants and is the main component of some sugars (such as sucrose, maltose, starch, cellulose, etc.), and is the main energy substance in plants. Glucose is the product of photosynthesis and the substrate of respiration. It provides energy for plant growth and development and can enhance plant stress resistance. Glucose metabolism is the center of the whole plant metabolism, which communicates the metabolism of proteins, lipids and secondary products. [0003] At present, in the research of plant physiology, colorimetric method, DNS reagent reducing sugar determination method, liquid chromatography and so on are mostly used for glucose detection. This type of method ne...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/48G01N27/327
Inventor 胡叶王成王晓冬侯佩臣何璐璐周航任鹏路文超宋鹏潘大宇
Owner BEIJING RES CENT FOR INFORMATION TECH & AGRI
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