Method for inducing vein burst of Newhall navel orange in magnesium deficiency condition

A technology of hormone umbilicus and magnesium deficiency, applied in the directions of botanical equipment and methods, application, magnesium fertilizer, etc., can solve the problems of leaf loss, leaf yellowing, magnesium deficiency, etc., and achieve the effect of easy to master and simple operation.

Active Publication Date: 2016-03-02
CITRUS RES INST SOUTHWEST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, after long-term treatment with boron application, the symptoms of yellowing leaves and swollen veins of Newhall navel oranges in Jiangnan production area have not been eliminated.
Since 2008, the inventor has investigated the orchards with symptoms of swollen veins (accompanied by leaf yellowing) in Gannan navel orange producing areas, and analyzed the status of the total nutritional elements of the leaves, showing that the boron content of the leaves of most orchards has been in an appropriate and moderate level. High levels, but widespread magnesium deficiency or deficiency, preliminarily judged that this symptom is caused by magnesium deficiency
The establishment of relevant methods mainly faces the following problems: first, Newhall navel oranges are grafted seedlings, and it is difficult to ensure their long-term normal survival by hydroponics; second, there has been a long-term controversy about whether sand culture methods will ensure magnesium deficiency in citrus; Third, there is currently no magnesium-deficient culture solution formula that can ensure the balance of nutrients in Newhall navel orange leaves; fourth, since magnesium is a medium element in plants, its proportion in citrus leaves is higher than that in other parts, so it is difficult to Severe loss of leaves in a short period of time

Method used

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  • Method for inducing vein burst of Newhall navel orange in magnesium deficiency condition
  • Method for inducing vein burst of Newhall navel orange in magnesium deficiency condition
  • Method for inducing vein burst of Newhall navel orange in magnesium deficiency condition

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Example 1 Initial Experiment of Magnesium Deficiency Inducing Newhall Navel Orange Leaf Vein Burst

[0043] Select grafted Newhall navel orange seedlings with a diameter of about 0.5 cm and a height of about 30 cm, and cut the aboveground part of the plant to a height of about 20 cm, leaving 5 autumn leaves in 2010, and cutting the main root to about 5 cm. On March 23, 2011, the potted sand cultivation experiment was carried out. One plant per pot was added with 8 kg of quartz sand and perlite (volume ratio 1:1), and a tray was placed on the bottom of the pot. In the early stage, only deionized water was supplied for seedling hardening.

[0044] On April 15, 2011, magnesium deficiency stress culture was carried out. The culture medium uses Hoagland (1 / 2 concentration) and Aron full concentration formula nutrient solution:

[0045] The formula of the normal group is: Ca(NO 3 ) 2 4H 2 O2mmol·L -1 , KNO 3 3mmol·L -1 , NH 4 h 2 PO 4 0.5mmol·L -1 , MgSO 4 ·7H 2...

Embodiment 2

[0054] Example 2 Simple Magnesium Deficiency Induced Newhall Navel Orange Leaf Burst Experiment

[0055] Such as figure 1 As shown, select the 2-year-old Newhall navel orange grafted seedlings with a rootstock diameter of about 1.5cm and a height of about 35cm, and cut the aboveground part of the plant to about 20cm, leaving five 2-year-old leaves (2013 autumn leaves) , The main root is cut to about 5cm. On March 18, 2014, the potted sand culture experiment was carried out. One plant per pot was added with 8 kg of quartz sand and perlite (volume ratio 1:1), a tray was placed on the bottom of the pot, and deionized water was supplied for hardening for 15 days.

[0056] On April 3, 2014, 1 / 4 of the improved Hoagland and Aron formula nutrient solutions were used to culture the grafted seedlings for 30 days. Its formula is: Ca(NO 3 ) 2 4H 2O0.8125mmol·L -1 , KNO 3 0.1575mmol·L -1 , NH 4 NO 3 0.125mmol·L -1 , KH 2 PO 4 0.125mmol·L -1 , MgSO 4 ·7H 2 O0.375mmol·L -1 ...

Embodiment 4

[0068] Example 4 Microscopic changes of leaf main vein and lateral vein tissue structure

[0069] Observation of leaf microstructure: taking the 2-year-old autumn shoot old leaves of Example 2 as objects, the conventional paraffin section method was adopted. The thickness of the main vein slices was 6 μm, and that of the lateral veins was 5 μm; they were counter-stained with safranin and fast green, sealed with neutral resin, and the fiber structure changes of the main veins and lateral veins were observed. The pictures were acquired by OLMPUSBX-51 microscope imaging system.

[0070] Test results such as Figure 4 shown. Among them, A: main vein at the 2nd month; B: main vein at the 4th month; C: primary phloem cell degeneration (red arrow); D: main vein at the 6th month, phloem cells grew significantly (black arrow) ;E: phloem cell enlargement (black arrow), cell disintegration (red arrow); F: main vein burst; G: lateral vein at the second month; H: lateral vein at the fou...

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Abstract

The invention provides a method for inducing vein burst of a Newhall navel orange in a magnesium deficiency condition, which comprises the steps of (a) selecting 2-years old trifoliate stock Newhall navel orange grafted seedlings every spring, and supplying deionized water after pruning root, stem and leaves for sand culturing colonization and hardening seedling; (b) culturing the grafted seedlings with a 1/4 improved Hoagland and Aron formula nutrient solution; (c) performing magnesium-deficiency culture to the grafted seedlings with the improved Hoagland and Aron formula nutrient solution; and (d) controlling the growth of the new shoot by pruning, promoting the veins of the 2-years old leaves to burst; and disclosing each nutrient solution formula. Through adoption of the method, the 2-years old leaves of the 2-years old Newhall navel orange plant are short of magnesium severely in a short period; and the phenomenon of substantial and typical veins burst occurs at the same year; and the operation is simple and the method can be easily grasped.

Description

technical field [0001] The invention relates to a method for inducing the bursting of veins of Newhall navel orange leaves by magnesium deficiency. Background technique [0002] China has become the world's largest producer of Newhall navel oranges, and Gannan is the largest producer of Newhall navel oranges in China. In recent years, many Newhall navel orange orchards in southern Jiangxi have suffered from large areas of leaf deficiency, yellowing and swollen veins, especially in hilly red soil and sandy soil orchards with less organic fertilizer application. Usually, swollen and cracked leaf veins are considered to be a typical symptom of boron deficiency in citrus, so boron application has been used in the production of Gannan Newhall navel oranges. However, after long-term treatment with boron application, the symptoms of yellowing leaves and swollen veins of Newhall navel oranges in southern Jiangxi production areas have not been eliminated. Since 2008, the inventor h...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G17/00A01G1/06C05G1/06
CPCA01G2/30A01G17/005C05B7/00C05C1/00C05C5/02C05C5/04C05D5/00C05D9/02
Inventor 凌丽俐彭良志淳长品曹立江才伦付行政
Owner CITRUS RES INST SOUTHWEST UNIV
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