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Bacillus thuringiensis capable of killing lepidopterous insects, plant nematodes and coleosporium and application

A technology of thuringiensis spores and lepidopteran insects, applied in the directions of nematicides, botanical equipment and methods, applications, etc.

Inactive Publication Date: 2016-03-30
ENVIRONMENT & PLANT PROTECTION INST CHINESE ACADEMY OF TROPICAL AGRI SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In particular, no one has screened out a strain of Bt bacteria from natural resources, especially plant tissue impurities, which has three plant protection functions at the same time, and can efficiently kill a variety of Lepidoptera insects, plant parasitic nematodes and plant coleoptera Rust

Method used

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  • Bacillus thuringiensis capable of killing lepidopterous insects, plant nematodes and coleosporium and application
  • Bacillus thuringiensis capable of killing lepidopterous insects, plant nematodes and coleosporium and application
  • Bacillus thuringiensis capable of killing lepidopterous insects, plant nematodes and coleosporium and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1: Testing the biocidal activity of Bacillus thuringiensis Bt00-50-5 inoculum (comprising crystals, spores, supernatant extrasporial proteins and few vegetative bodies).

[0032] The insecticidal activity test of Bacillus thuringiensis Bt00-50-5 bacterial agent to Lepidoptera insects is as follows:

[0033] Insects or nematodes tested: sunflower band moth, cabbage butterfly, cabbage caterpillar, cabbage looper, yellow cutworm, elm looper, European corn borer, oriental corn borer.

[0034] The sunflower banded moth adopts the method of measuring one worm per tube: the larvae are raised with artificial feed; a hole is punched at the top of each tube, a foam strip with micropores is placed, and Bt bacterial agent suspensions of different concentrations are dripped, containing 0.05% TritonX- 100, one second instar larva was added to each tube, and the number of dead larvae was recorded after 3 days.

[0035] Cabbage leaf dipping method was used to cut the leaves of...

Embodiment 2

[0059] Embodiment 2: Bacillus thuringiensis Bt00-50-5 inoculum efficiently prevents and treats plant diseases, insect pests

[0060] Dilute 40 times with the suspension bacterial agent of Bacillus thuringiensis Bt00-50-5 liquid fermentation, and add 0.05% Triton-X100 before spraying,

[0061] Treatment group D: Spray Bt00-50-5 bacterial agent on the Chinese cabbage growing in the field, and spray it on the 10th day after the Chinese cabbage seedlings are unearthed, once every 7 days, a total of 3 times;

[0062] Control group E: replace the Bt00-50-5 bacterial agent with tap water and spray growing Chinese cabbage under the same conditions;

[0063] Control group F: The growing cabbage was sprayed with chemical pesticide instead of Bt00-50-5 bacterial agent under the same conditions.

[0064] On the 45th day, observe the control effect, the leaves of Chinese cabbage sprayed with Bt00-50-5 bacterial agent in the field do not find holes bitten by insects and symptoms of plant d...

Embodiment 3

[0065] Embodiment 3: the Bacillus thuringiensis Bt00-50-5 bacterial agent of different doses is to banana spiral nematode H.Multicinctus preventive effect

[0066] Efficacy of different doses of Bacillus thuringiensis Bt00-50-5 against H. multicinctus. The fermented stock solution of Bt00-50-5 bacteria diluted 10, 20 and 40x was used twice for root irrigation in the field. After 40 days, observe the nematode killing effect of Bacillus thuringiensis Bt00-50-5, the effect is as follows: image 3 . Specific method: Take soil samples from the banana rhizome and bring them back to the laboratory. After mixing well, take 200g soil sample and 2 plastic pots of the same size, place the one with mesh holes on the upper part and spread two layers of handkerchief paper on the bottom, put the soil sample on it, fill the lower pot with 400ml water, and let it stand overnight Finally, sieve the aqueous solution containing nematodes in the lower plastic basin (the upper layer is 100 mesh,...

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Abstract

The invention discloses bacillus thuringiensis. The bacillus thuringiensis is obtained from sunflower tissue fragment impurities of a natural material through screening. The preservation number is CGMCC No.11769; the preservation unit is China General Microbiological Culture Collection Center; the preservation address is No.3, Countyard 1, Beichen West Road, Chaoyang District, Beijing. The bacillus thuringiensis Bt 00-50-5 strain can produce elliptic spores, rhombus crystals and more than 8 extracellular proteins, wherein the crystals and the extracellular proteins have high toxicity and can efficiently kill the lepidopterous insects, the plant parasitic nematodes and the plant coleosporium. The bacillus thuringiensis Bt 00-50-5 capable of killing the plant coleosporium is obtained through screening at home and abroad for the first time and has the best plant disease and insect pest control effect.

Description

technical field [0001] The invention relates to a plant protection agent, in particular to a bacillus thuringiensis which can efficiently prevent and control lepidopteran insects, plant parasitic nematodes and plant sheath rust fungus. Background technique [0002] Bacillus thuringiensis (Bt) is an entomopathogenic bacterium, a Gram-positive bacillus, and a well-known pest biocontrol bacterium at home and abroad. Because it is toxic to specific insects, and the toxicity varies depending on the type of insect. Insecticides prepared by Bacillus thuringiensis are safe bacterial insecticides that are friendly to humans and the environment, and Bt insecticides have been widely used at present. Compared with chemical pesticides, the cost of using Bt insecticides to control pests is high. If ultra-efficient bacterial insecticides are used in biological control, their dosage and cost can be reduced. Therefore, it is very important to screen and obtain highly effective insecticida...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/02A01P7/04A01P5/00A01P3/00C12R1/07
CPCA01N63/10C12N1/205C12R2001/075
Inventor 白成龙海波孙燕芳刘丽平易克贤
Owner ENVIRONMENT & PLANT PROTECTION INST CHINESE ACADEMY OF TROPICAL AGRI SCI
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