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Sugar solution production method

A manufacturing method and sugar solution technology, which can be applied to methods using microorganisms, sugar production, and sugar solution boiling/evaporation, etc., and can solve the problems of cost, input of a large amount of energy, and low removal efficiency.

Active Publication Date: 2019-10-08
TORAY IND INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, in the hydrolysis of cellulose-containing biomass, there is a problem that sugars such as glucose and xylose are decomposed simultaneously with the decomposition of cellulose or hemicellulose components, and furfural, hydroxymethyl, etc. are also produced. Furan compounds such as furfural, or by-products such as organic acids such as formic acid, acetic acid, and levulinic acid
However, in such a method of evaporative removal, there is a problem that the removal efficiency of fermentation inhibitors such as organic acids with low boiling points is low depending on the boiling point of the fermentation inhibitors, and a large amount of energy must be invested in order to obtain sufficient removal efficiency.
[0007] In addition, there is also a method of ion exchange to remove fermentation inhibitors (Patent Document 7), but there is a problem in terms of cost; there is also a method of adsorption removal using woody carbides, that is, activated carbon, etc., but there are cases where the removal object is limited to hydrophobic compounds. Problem (Patent Document 8)
[0008] In addition, a method of using a nanofiltration membrane or a reverse osmosis membrane to remove fermentation inhibitors as the permeate of the membrane is also considered, but there are problems of requiring additional separation equipment and energy input for removal (Patent Document 9)

Method used

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Examples

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Embodiment

[0109] Examples are given below to describe the present invention in detail. However, the present invention is not limited to these.

reference example 1

[0110] (Reference Example 1) Measurement of Sugar Concentration

[0111] The concentrations of glucose and xylose contained in the sugar solution were quantified by comparison with standard products under the HPLC conditions shown below.

[0112] Column: Luna NH 2 (manufactured by Phenomenex)

[0113] Mobile phase: Milli-Q:acetonitrile=25:75 (flow rate 0.6mL / min)

[0114] Reaction solution: no

[0115] Detection method: RI (differential refractive index)

[0116] Temperature: 30°C.

reference example 2

[0117] (Reference Example 2) Analysis of Fermentation Inhibiting Substances

[0118] Aromatic compounds and furan compounds contained in the hydrolyzate were quantified by comparison with standard samples under the HPLC conditions shown below. In addition, each analysis sample was centrifuged at 3500G for 10 minutes, and the supernatant was used for the analysis described below.

[0119] Column: Synergi HidroRP 4.6mm×250mm (manufactured by Phenomenex)

[0120] Mobile phase: Acetonitrile-0.1%H 3 PO 4 (flow rate 1.0mL / min)

[0121] Detection method: UV (283nm)

[0122] Temperature: 40°C.

[0123] Acetic acid and formic acid were quantified by comparison with standard products under the HPLC conditions shown below. In addition, each analysis sample was subjected to centrifugation at 3500G for 10 minutes, and the supernatant fraction was used for the analysis described below.

[0124] Column: Shim-Pack and Shim-Pack SCR101H (manufactured by Shimadzu Corporation) in series ...

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Abstract

Although various fermentation inhibitors are contained in the aqueous sugar solution obtained from biomass, they can be removed easily and at low cost by treating the aqueous sugar solution with microorganisms that do not have the ability to assimilate glucose and xylose or crude enzymes derived from the microorganisms. Fermentation inhibitory substances derived from biomass.

Description

technical field [0001] The present invention relates to a method for producing sugar liquor from biomass. Background technique [0002] Fermentative production processes of sugar-based chemicals are used in the production of various industrial raw materials. As the sugar used as the raw material for this fermentation, sugar derived from edible raw materials such as sugarcane, starch, and sugar beets is currently used industrially, but from the ethical perspective of the skyrocketing price of edible raw materials caused by the increase in the world population in the future, or competition with food The process of efficiently producing sugar liquid from recycled non-edible resources, that is, cellulose-containing biomass, or the process of efficiently converting the obtained sugar liquid into industrial raw materials is a future issue. [0003] As a method for producing sugar liquid from cellulose-containing biomass, a method of producing sugar liquid by acid hydrolysis of ce...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/14C12P7/06C12P19/02
CPCC12P19/02C12P19/14C12N1/22C12P7/10C12P2203/00Y02E50/10C12P7/14C13B20/002C13B30/002C13B50/00C13K1/04C13K5/00C13K13/00C13K13/002
Inventor 栗原宏征石冢裕子山田胜成
Owner TORAY IND INC
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