Sugar solution production method
A manufacturing method and sugar solution technology, which can be applied to methods using microorganisms, sugar production, and sugar solution boiling/evaporation, etc., and can solve the problems of cost, input of a large amount of energy, and low removal efficiency.
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[0109] Examples are given below to describe the present invention in detail. However, the present invention is not limited to these.
reference example 1
[0110] (Reference Example 1) Measurement of Sugar Concentration
[0111] The concentrations of glucose and xylose contained in the sugar solution were quantified by comparison with standard products under the HPLC conditions shown below.
[0112] Column: Luna NH 2 (manufactured by Phenomenex)
[0113] Mobile phase: Milli-Q:acetonitrile=25:75 (flow rate 0.6mL / min)
[0114] Reaction solution: no
[0115] Detection method: RI (differential refractive index)
[0116] Temperature: 30°C.
reference example 2
[0117] (Reference Example 2) Analysis of Fermentation Inhibiting Substances
[0118] Aromatic compounds and furan compounds contained in the hydrolyzate were quantified by comparison with standard samples under the HPLC conditions shown below. In addition, each analysis sample was centrifuged at 3500G for 10 minutes, and the supernatant was used for the analysis described below.
[0119] Column: Synergi HidroRP 4.6mm×250mm (manufactured by Phenomenex)
[0120] Mobile phase: Acetonitrile-0.1%H 3 PO 4 (flow rate 1.0mL / min)
[0121] Detection method: UV (283nm)
[0122] Temperature: 40°C.
[0123] Acetic acid and formic acid were quantified by comparison with standard products under the HPLC conditions shown below. In addition, each analysis sample was subjected to centrifugation at 3500G for 10 minutes, and the supernatant fraction was used for the analysis described below.
[0124] Column: Shim-Pack and Shim-Pack SCR101H (manufactured by Shimadzu Corporation) in series ...
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