39results about "Purification using microorganisms/enzymes" patented technology

Method for extracting oils, proteins and fermentable sugars from vegetable material in an aqueous medium, includes:a) adding water to the vegetable material;b) adding an enzyme mixture containing at least one cellulase, at least one hemicellulase, and at least one pectinase, the ratio between the pectinase activity and the cellulase activity being at least 0.14, and the ratio between the pectinase activity and the hemicellulase activity being at least 7.10−3, the pectinase activity being less than 120 μmol / min / mL;c) incubating the vegetable material and the enzyme mixture with stirring to release oils, proteins and fermentable sugars in the reaction medium;d) separating the reaction medium to obtain free oil, an aqueous phase containing proteins and fermentable sugars, and a solid phase;e) optionally separating and recycling an emulsion of free oil or aqueous phase, to the medium;f) separating the proteins and fermentable sugars from the aqueous phase.

The invention provides a cane sugar alcohol co-production low-carbon and environmental-protection sugaring method based on a solid fermentation technique. The method comprises a low-energy-consumption high-quality cane sugar production procedure and a procedure of fermenting and transforming sugar solid, which is residual in molasses and bagasse, into alcohol. By-products can be utilized by the following steps: carbon dioxide generated in the solid fermentation procedure is taken as a clarifying agent applied to cane juice purification; fermentation dregs generated in a solid distillation procedure can be applied to power generation in a bagasse power plant or fodder; waste steam from the bagasse power plant is applied to alcohol separation and / or cane juice in the solid distillation procedure. According to the low-carbon and environmental-protection sugaring method, cane sugar in sugarcane can be utilized to the maximum extent, the production costs of the cane sugar and the energy consumption are reduced, sugar is produced, and alcohol fuel is also produced; due to a solid fermentation technique, the quantity of organic sewage generated in the production procedure is reduced; the by-products, such as the carbon dioxide, the fermentation dregs and the waste steam, can be comprehensively utilized.

Although various fermentation inhibitors are contained in the aqueous sugar solution obtained from biomass, they can be removed easily and at low cost by treating the aqueous sugar solution with microorganisms that do not have the ability to assimilate glucose and xylose or crude enzymes derived from the microorganisms. Fermentation inhibitory substances derived from biomass.

Conventionally, sugarcane processing avoids leaving residual sucrose in the bagasse, since the bagasse will be burned and the value of the sucrose would be lost. However, when coupled with a Green Power+® process to extract hemicelluloses, sucrose may also be extracted and recovered from the bagasse. In some variations, a process includes mechanically treating a feedstock to generate a sucrose-rich stream and lignocellulosic material that intentionally retains a significant amount of the initial sucrose in the feedstock; extracting the lignocellulosic material with steam and / or hot water to produce cellulose-rich solids and an extract liquor containing hemicellulosic oligomers and sucrose; and then hydrolyzing the hemicellulosic oligomers into a hemicellulose sugar stream. Each of the sucrose-rich stream and the hemicellulose sugar stream (containing the starting residual sucrose) may be recovered or further processed (e.g., fermented to ethanol). Similar processes are possible with energy cane, sugar beets, and energy beets.