Method for producing saccharide solution
A manufacturing method, technology of sugar solution, applied to methods using microorganisms, production of sugar, boiling/evaporation of sugar solution, etc., can solve problems of cost, remove object limitation, and invest a lot of energy
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[0108] Examples are given below to describe the present invention in detail. However, the present invention is not limited to these.
reference example 1
[0109] (Reference Example 1) Measurement of Sugar Concentration
[0110] The concentrations of glucose and xylose contained in the sugar solution were quantified by comparison with standard products under the HPLC conditions shown below.
[0111] Column: LunaNH 2 (manufactured by Phenomenex)
[0112] Mobile phase: Milli-Q:acetonitrile=25:75 (flow rate 0.6mL / min)
[0113] Reaction solution: no
[0114] Detection method: RI (differential refractive index)
[0115] Temperature: 30°C.
reference example 2
[0116] (Reference Example 2) Analysis of Fermentation Inhibiting Substances
[0117] Aromatic compounds and furan compounds contained in the hydrolyzate were quantified by comparison with standard samples under the HPLC conditions shown below. In addition, each analysis sample was centrifuged at 3500G for 10 minutes, and the supernatant was used for the analysis described below.
[0118] Column: Synergi Hidro RP4.6mm×250mm (manufactured by Phenomenex)
[0119] Mobile phase: Acetonitrile-0.1%H 3 PO 4 (flow rate 1.0mL / min)
[0120] Detection method: UV (283nm)
[0121] Temperature: 40°C.
[0122] Acetic acid and formic acid were quantified by comparison with standard products under the HPLC conditions shown below. In addition, each analysis sample was subjected to centrifugation at 3500G for 10 minutes, and the supernatant fraction was used for the analysis described below.
[0123] Column: Shim-Pack and Shim-Pack SCR101H (manufactured by Shimadzu Corporation) in series ...
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