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A kind of preparation method and application of extracting alkaloid from mountain pepper root

A technology of mountain pepper root and alkaloids, which is applied in the field of natural plant extraction and separation, can solve the problems of many extraction liquid filtration steps, low efficiency of active ingredients, and long time-consuming heating treatment, etc., to achieve good leaching effect, convenient extraction, and easy scale-up Effect

Inactive Publication Date: 2018-08-21
HUNAN UNIV OF SCI & ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing techniques for extracting and purifying the total alkaloids of the mountain pepper mainly have the following deficiencies: (1) using high-concentration alcohol solution as the extraction medium, the cost is high, and the extraction is not complete enough; while water is used as the extraction medium, although Economical, but the efficiency of extracting active ingredients is low, and it is difficult to effectively extract the active ingredients of alkaloids in the root of mountain pepper
(2) Traditional extraction methods such as decoction, reflux, dipping, etc., the extraction process involves heat treatment or takes too long, and there are many steps for filtering the extract, and the yield is not ideal

Method used

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  • A kind of preparation method and application of extracting alkaloid from mountain pepper root
  • A kind of preparation method and application of extracting alkaloid from mountain pepper root
  • A kind of preparation method and application of extracting alkaloid from mountain pepper root

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Experimental program
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Effect test

Embodiment 1

[0035]1) Raw material processing: Take 600g of mountain pepper root and dry it in the shade until the water content is 3-8%, then crush it, pass through a 20-80 mesh sieve to get coarse powder, and set aside; 2) Percolation and extraction of acidic alcohol aqueous solution: take step 1) Prepared dry coarse powder, add 1.5 times the amount of acidic alcohol aqueous solution with a pH value of 1-2, wherein the concentration of alcohol is 50%, soak in the percolation bucket after 3-6 hours, and use 15 times the amount of the above-mentioned acidic alcohol The aqueous solution is extracted by percolation, the flow rate is adjusted, and the percolation extract is collected at a flow rate of 10-20mL / min until the alkaloids in the mountain pepper root powder are extracted; using thin-layer chromatography, it appears brown-black at 254nm Spots, bismuth potassium iodide solution is orange-yellow, add alkaline water to the obtained extract to adjust the pH value to 9-10, and then add org...

Embodiment 2

[0037] 1) 1) Raw material processing: Take 600g of mountain pepper root and dry it in the shade until the water content is 3-8%, then crush it, pass through a 20-80 mesh sieve to get coarse powder, and use it for later use; 1) For the prepared dry coarse powder, add 1 times the amount of acidic alcohol solution with a pH value of 1-2, wherein the concentration of alcohol is 30%, soak it for 3-6 hours and put it into the percolation barrel, and use 10 times the amount of the above-mentioned The acidic alcohol aqueous solution is extracted by percolation, the flow rate is adjusted, and the percolation extract is collected with a flow rate of 10-20mL / min until the alkaloid in the peppercorn root powder is extracted cleanly; Brown-black spots, bismuth potassium iodide solution is orange-yellow, add alkaline water to the obtained extract to adjust the pH value to 9-10, and then add organic solvent to extract 2-6 times to obtain the extract; wherein, the extract and organic solvent ...

Embodiment 3

[0039] 1) Raw material processing: Take 600g of mountain pepper root and dry it in the shade until the water content is 3-8%, then crush it, pass through a 20-80 mesh sieve to get coarse powder, and set aside; 2) Percolation and extraction of acidic alcohol aqueous solution: take step 1) Prepared dry coarse powder, add 2 times the amount of acidic alcohol aqueous solution with a pH value of 1-2, wherein the concentration of alcohol is 80%, soak in the percolation bucket after 3-6 hours, and use 18 times the amount of the above acidic alcohol The aqueous solution is extracted by percolation, the flow rate is adjusted, and the percolation extract is collected at a flow rate of 10-20mL / min until the alkaloids in the mountain pepper root powder are extracted; using thin-layer chromatography, it appears brown-black at 254nm Spots, bismuth potassium iodide solution is orange-yellow, add alkaline water to the obtained extract to adjust the pH value to 9-10, and then add organic solven...

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Abstract

The invention discloses a preparation method for alkaloids by performing extraction on lindera glauca root and uses thereof. The preparation method comprises the following steps: 1) processing raw materials; 2) performing percolating extraction by using an acidic alcohol aqueous solution; 3) preparing a lindera-glauca-root total alkaloids extractive concentrate; 4) preparing an eluent; and 5)-8) preparing and purifying the compounds. By employing the acidic alcohol aqueous solution as the extraction solvent, the extraction solvent can be repeated recovered for utilization, and cost is saved. Percolating extraction is employed, the leachate can reach a relatively high concentration, and extraction efficiency can be improved. The method employs normal-temperature operation, does not need heating, the solvent usage amount is less, the filtration requirement is relatively low, the separation operation process is simplified, the extractive is less in impurities, extraction efficiency is high, and the solvent usage amount is less.

Description

technical field [0001] The invention belongs to the technical field of extraction and separation of natural plants, and in particular relates to a preparation method for extracting alkaloids from peppercorn roots and an application thereof. Background technique [0002] Lauraceae Lauraceae Lindera Thunb . There are about 100 species of plants in the world, distributed in Asia and North America in warm and tropical regions. There are 40 species, 9 variants and 2 variants in our country. Pepper is one of the genus with more species and wider distribution in Lauraceae in my country. mountain pepper Lindera glauca (Siebold & Zucc.) Blume, born on hillsides, forest margins, and roadsides below an altitude of about 900 meters. In my country's south of Kunyu Mountain in Shandong, south of Song County in Henan, south of Yun County in Shaanxi, and Gansu, Shanxi, Jiangsu, Anhui, Zhejiang, Jiangxi, Fujian, Taiwan, Guangdong, Guangxi, Hubei, Hunan, Sichuan and other provinces Wide...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D221/18A61K31/473A61P35/00
CPCC07D221/18
Inventor 刘婷
Owner HUNAN UNIV OF SCI & ENG
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