Preparation and preservation method of amniotic membrane biological agent

Abstract

The invention provides a preparation and preservation method of an amniotic membrane biological agent. The method includes the first step of raw material selection and pretreatment, the second step of inactivation treatment of viruses, the third step of preparation, the fourth step of dyeing and the fifth step of preservation. Compared with the prior art, the method has following advantages that the preparation method is simple, raw materials are wide in source, large-scale industrial production can be achieved, prepared decellularized amniotic membranes are low in treatment strength and good in decellularization effect, and dense collagenous structures of natural amniotic membranes can be fully maintained. Amniotic membrane features are optimized easily, and important technical support can be provided for tissue engineering amniotic membrane industrialization.

Description

technical field [0001] The invention relates to the preparation and preservation method of amniotic membrane biological preparations, belonging to the technical field of medical biological materials. Background technique [0002] Tissue adhesions are abnormal structures formed by the combination of connective tissue fibrous bands and adjacent tissues or organs, which can cause various postoperative complications: for example, adhesions after tendon repair, seriously affecting the recovery of the function of the surgical site, and even Can cause loss of local function. [0003] Amniotic membrane is the inner layer of human fetal membranes, including the thickest basement membrane and avascular interstitium in the human body. The amniotic membrane has low immunogenicity and can reduce inflammatory reactions and inhibit fibrous tissue proliferation. It can be used as an ideal Tissue engineering repair materials. [0004] Patents CN1369555A and CN102327640A respectively use re...

AI Technical Summary

Problems solved by technology

[0004] Patents CN1369555A and CN102327640A respectively use reagents such as trypsin and TBS-SDS (TBS buffer containing sodium dodecyl sulfate) to decellularize the amnion. The amnion material prepared is relatively thin, easy to curl, and has poor adhesion. easy to slide off

In order to overcome the above disadvantages, the patent CN100368534C adopts cross-linking technology to prepare composite biologically derived amnion to improve its operability. After the step, the natural structure of the amniotic membrane is greatly damaged, resulting in irregular arrangement of collagen bundles and fracture of collagen fibers.

Aiming at the shortage of bio-derived amniotic membrane, patent CN1943796A discloses a method for making an irradiation cross-linked porous network decellularized amniotic membrane hydrogel auxiliary material, and proposes the concept of hydrogel dressing, but uses trypsin and ethylenediaminetetraacetic acid (EDTA) decellularizes the amniotic membrane, which can easily destroy the natural structure of the amniotic membrane, cause collagen fiber breakage, and reduce the mechanical properties of the material. The safety of the product is often accompanied by some side reactions during the combination process, and the two-time irradiation process will greatly destroy the spatial collagen structure of the natural amniotic membrane

Patent CN101040616A soaks amniotic membrane and growth factor to prepare drug amniotic membrane, but it still does not improve the operational shortcomings of single-layer amniotic membrane being thin and easy to curl, and the growth factor after soaking is only attached to the surface of the amniotic membrane, and the slow-release performance is poor