Water pressure-based micro-nano electric single-cell control system

Abstract

The invention discloses a micro-nano-scale electric unicell control system based on water pressure. A linear mobile platform supporting frame is perpendicularly fixed to a workbench. The bottom face of a U-shaped frame is fixed to an adapter plate. A linear mobile platform is installed on the side face of the linear mobile platform supporting frame. The adapter plate is fixed to a mobile table of the linear mobile platform. A liquid container is contained in the U-shaped frame. Two thread adapters on an upper cover of the liquid container are connected with two-way valves respectively. A third thread adapter on a lower cover of the liquid container is connected to one end of a probe holder, and the other end of the probe holder is provided with a probe. The probe holder is clamped into a spring clamping groove in a clamp of the probe holder and fixed to the lower end of the other adapter plate. The upper end of the other adapter plate is connected to a microoperation platform. The three-axis precision can reach about 7 nm, the stroke is 20 mm, and damage to living cells is small. Cell control is stable, and the precision of the linear mobile platform for controlling water pressure is 0.25 micrometer. The micro-nano-scale electric unicell control system is used for unicell microoperation experiments, and can be easily integrated to an experiment system based on unicell microoperation.

Description

technical field [0001] The invention relates to a single-cell manipulation system, in particular to a water pressure-based micro-nano electric single-cell manipulation system. Background technique [0002] With the development of biophysical methods in recent years, many novel and efficient methods have emerged, and these emerging systems often need to be skillfully integrated into new systems using formed sub-modules. In many cell experiments, due to the small size of cells, real-time observation needs to be based on microscope and CCD. If you want to observe the changes of single cells under various conditions (such as morphology, fluorescence, membrane potential, etc.), you first need to fix the single cells and require the ability to move the cells as needed. Thus, cell manipulation systems are the basis for many single-cell experimental approaches. At present, there are few such formed systems on the market, especially cell manipulation systems that can be easily and ...

AI Technical Summary

Problems solved by technology

First of all, considering that many single-cell experiments have anti-vibration requirements, the use of the air pump will obviously affect the experimental process; secondly, the precision of the suction force for fixed cells is also extremely high. Cells are easy to escape; again, the small size of cells often requires high-power objective lenses for observation, and the field of view of high-power objective lenses is usually small, making it difficult to achieve extremely precise micro-nano level micromanipulation of cells

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