Functional molecular marker for corn germination potential gene ZmGLP and application of functional molecular marker

A technology of functional molecules and germination potential, applied in the field of genetic engineering, can solve problems such as differences in germination rate and achieve the effect of improving efficiency

Active Publication Date: 2016-04-20
HENAN AGRICULTURAL UNIVERSITY
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

[0003] Although the germination rate of seeds is the result of the joint action of environmental and genetic factors, its own genetic characteristics are the main reason for the differences in germination rates among different varieties

Method used

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  • Functional molecular marker for corn germination potential gene ZmGLP and application of functional molecular marker
  • Functional molecular marker for corn germination potential gene ZmGLP and application of functional molecular marker
  • Functional molecular marker for corn germination potential gene ZmGLP and application of functional molecular marker

Examples

Experimental program
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Embodiment 1

[0051] Example 1: Development of functional molecular markers of corn seed germination potential gene ZmGLP

[0052] (1) Sequence structure analysis of maize ZmGLP gene

[0053] The inventors analyzed and studied the differentially expressed proteins in the germination process of Nongda 108, Zhengdan 958, Xianyu 335, Yuyu 22, Jundan 20 and Jundan 18 and their corresponding parental seeds. On the above, the gi|195606798 protein that affects the germination characteristics of seeds was obtained, the reference sequence of the ZmGLP gene was extracted, and multiple pairs of amplification and sequencing primers were designed to amplify the ZmGLP gene of 40 maize inbred lines. Two pairs of primers including UTR, exon, intron and 3'UTR (cupinF2: CGTAGCACCAGAAAAGAAATGTA / cupinR1: CACAGGTAGGCGGTAGCAGC, cupinF6: GGGGAAGCGAAGGTTGGGTAT / cupinR7: ACAAACGGTCACTGCGGGAC) were used to amplify and sequence the ZmGLP gene sequences of other inbred lines .

[0054] The promoter region of the gene...

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Abstract

The invention discloses a functional molecular marker for a corn germination potential gene ZmGLP. The sequences of PCR primers of the functional molecular marker are as followed: IDF:TATTCCCGAACGGGTCCC and IDR:CGAGGTGGTGAAGCCGAAGAA; the sequences of internal reference primers of the functional molecular marker are as followed: CF:GGGGAAGCGAAGGTTGGGTAT and CR:CGTTGAAGGCACGGGTAAGC. The functional molecular marker for the corn germination potential gene ZmGLP does not depend on molecular genetic mapping, the efficiency of molecular marker-assisted selection can be greatly improved through the functional marker, the functional molecular marker can be directly applied to practice, and a technical support is supplied to selection breeding of good inbred lines or hybrids with the high germination rate through a molecular marker-assisted selection method.

Description

technical field [0001] The invention belongs to the field of genetic engineering, and in particular relates to a functional molecular marker of a maize germination potential gene ZmGLP and its application in breeding. Background technique [0002] In corn production input, seed is the most basic and effective input, and also the most special means of production, which directly affects the economic interests of producers, operators and users. Among many indicators to measure seed quality, germination rate is the most direct and effective evaluation standard, which is of great significance for seed purchase, storage, transportation, etc., and is also the basis for determining the sowing amount in the field. Almost every year in my country's agricultural production, there are cases of corn production reduction due to low seed germination rate. In recent years, some large multinational seed companies such as Pioneer and Syngenta have successively entered the Chinese market. The ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 付志远汤继华丁冬李卫华李浩川薛亚东郭占勇
Owner HENAN AGRICULTURAL UNIVERSITY
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