A rapid method for measuring liposome encapsulation volume

A technology for rapid determination of the encapsulated volume, which is applied in volume measurement instruments/methods, liquid/fluid solid measurement, chemical analysis by titration, etc. It can solve complex and restrictive liposome encapsulated volume determination methods problem, to achieve the effect of low test cost, high accuracy and simple equipment

Active Publication Date: 2019-01-18
DALIAN NATIONALITIES UNIVERSITY
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AI Technical Summary

Problems solved by technology

[0004] In order to solve the complex and restrictive problem of liposome encapsulation volume measurement methods in the prior art, the present invention provides a rapid determination method of liposome encapsulation volume, which does not require centrifugation, dialysis, coagulation, etc. Gel filtration and other complex operations, directly through the most convenient acid-base titration technology to quickly determine the liposome encapsulation volume

Method used

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  • A rapid method for measuring liposome encapsulation volume
  • A rapid method for measuring liposome encapsulation volume
  • A rapid method for measuring liposome encapsulation volume

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Embodiment 1: use phosphatidylcholine (DOPC) to prepare liposome and measure liposome encapsulation volume

[0026] 1.1 Preparation of liposomes

[0027] Liposome preparation was carried out by thin film dispersion method. Weigh 0.0063 g of DOPC phospholipid and place it in a suitable container, dissolve it completely with 5 mL of chloroform, slowly evaporate to dryness on a rotary evaporator to evenly distribute the phospholipid on the inner surface of the container, and dry it in a vacuum dryer for more than 5 hours to remove chloroform. 4 mL of the solution for preparing liposomes was added to the dried phospholipid film, vibrated with a vortex oscillator, and then treated with an ultrasonic device to prepare liposomes.

[0028] 1.2 Acid-base titration of liposome solution

[0029] Get a fixed volume of liposome solution for acid-base titration, first measure the acidity and alkalinity of the liposome solution, and select a suitable acid-base indicator and standard...

Embodiment 2

[0035] Example 2: Preparation of liposomes using artificial lipid (2,3-dioleoyl-propyl)-trimethylamine (DOTAP) and determination of liposome encapsulation volume

[0036] 2.1 Preparation of liposomes

[0037] Liposome preparation was carried out by thin film dispersion method. Weigh 0.0063 g of DOTAP lipid and place it in a suitable container, dissolve it completely with 5 mL of chloroform, slowly evaporate it on a rotary evaporator to make the phospholipids evenly distributed on the inner surface of the container, and dry it in a vacuum dryer for more than 5 hours to remove chloroform . 4 mL of the solution for preparing liposomes was added to the dried phospholipid film, vibrated with a vortex oscillator, and then treated with an ultrasonic device to prepare liposomes.

[0038] 2.2 Acid-base titration of liposome solution

[0039] Get the liposome solution of fixed volume and carry out acid-base titration, liposome solution is weakly alkaline in the present embodiment, sele...

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Abstract

The invention discloses a rapid measurement method of the liposome encapsulation volume and particularly relates to optimization of a rapid measurement and preparation technology for the encapsulation volume of a liposome preparation in the field of drug production. The method comprises steps as follows: an acid-base indicator is added to a liposome solution, a standard solution is used for acid-base titration, and the usage amount V1 of the standard solution at the titration balanced point is recorded; the same quantity of a liposome solution is taken, a surfactant is added, then a standard solution is used for acid-base titration again, and the usage amount V2 of the standard solution at the titration balanced point is recorded; the liposome encapsulation volume is calculated according to the formula PIN(%)=(V2-V1) / V2. Compared with the prior art, the measurement method has the advantages that the method is simple, complex operation such as separation, dialysis, gel filtration and the like is not required, consumed time is short, the measurement result of the method is high in accuracy, equipment is simple, the application range is wide, and the method can be applied to optimization of a detection and preparation technology for liposome products and plays a key role in actual application of a liposome encapsulated water-soluble drug.

Description

technical field [0001] The invention relates to a method for rapidly measuring the encapsulation volume of liposomes, in particular to the rapid determination and preparation process optimization of the encapsulation volume of liposome preparations in the field of pharmaceutical manufacturing. Background technique [0002] Liposome drugs have become an important direction for the modification of current drug dosage forms. They have attracted much attention because of their good biocompatibility, the ability to change the distribution of encapsulated drugs in vivo and improve the therapeutic index of drugs. The use of liposomes for dosage form modification It can not only improve the solubility and utilization efficiency of drugs, reduce adverse drug reactions, but also carry out targeted drug delivery, effectively deliver drugs to the lesion to specifically kill tumors or lesion cells, and avoid systemic drug damage to the body. . [0003] The encapsulation volume refers to...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01F22/00G01N31/16
CPCG01F22/00G01N31/16
Inventor 刘宝全李春斌韩哲先王剑锋权春善范圣第
Owner DALIAN NATIONALITIES UNIVERSITY
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