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A countercurrent ultrasonic preparation method of propolis extract

A technology of propolis extract and countercurrent ultrasound is applied in the directions of medical preparations containing active ingredients, pharmaceutical formulations, unknown raw materials, etc., which can solve problems such as poor mass transfer effect and achieve the effect of shortening extraction time.

Active Publication Date: 2020-02-21
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] There have been reports on the method of ultrasonic multi-frequency combination extraction, such as a countercurrent pass-through ultrasonic device disclosed in the invention patent of CN102764620B. This method is that the material flow flows under many parallel ultrasonic heads. Compared with this method , the material flow does not flow in the opposite direction to the direction of the ultrasonic longitudinal wave, and the mass transfer effect is poor
Publication No. 102764619A discloses a sequential working multi-frequency non-contact and contact combined ultrasonic treatment equipment, CN102764621A sequential working multi-frequency contact ultrasonic-assisted extraction and ultrasonic-assisted reaction equipment are all static extraction equipment. Compared with this method, the material The flow does not flow in the opposite direction to the direction of the ultrasonic longitudinal wave, and the mass transfer effect is poor

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  • A countercurrent ultrasonic preparation method of propolis extract
  • A countercurrent ultrasonic preparation method of propolis extract
  • A countercurrent ultrasonic preparation method of propolis extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034]100 g of the raw material was frozen and pulverized until all passed through an 80-mesh sieve. According to the liquid-to-material ratio of 10:1 (mL:g), add edible ethanol aqueous solution with a concentration of 50% (v / v) for preparation, and the prepared liquid is pumped into the countercurrent ultrasonic extraction chamber with a peristaltic pump at a feed rate of 100mL / min , the flow rate is 10ml / min helium for protection and stirring, the ultrasonic frequency is 20kHz for treatment, the ultrasonic power is 500W, the ultrasonic work / intermittent time ratio is 1:10, the initial temperature of the feed liquid is 20°C, and the ultrasonic time is 5min. During the process of ultrasonic treatment, the material liquid is continuously stirred to ensure uniform ultrasonic treatment. After the extraction, the extract was refrigerated and centrifuged at 10000r / min to remove insoluble solid impurities. The supernatant was concentrated to extract by vacuum concentration. The su...

Embodiment 2

[0036] 1000 g of the raw material is frozen and pulverized until all pass through an 80-mesh sieve. According to the liquid-to-material ratio of 50:1 (mL:g), adding a concentration of 90% edible ethanol aqueous solution is prepared, and the prepared feed liquid is pumped into the countercurrent ultrasonic extraction chamber with a feed rate of 500mL / min by a peristaltic pump. 300ml / min helium is used for protection and stirring, ultrasonic frequency is 40kHz, ultrasonic power is 2000W, ultrasonic working / intermittent time ratio is 10:1, initial temperature of feed liquid is 60°C, and ultrasonic time is 25min. During the process of ultrasonic treatment, the material liquid is continuously stirred to ensure uniform ultrasonic treatment. After the extraction, the extract was refrigerated and centrifuged at 10000r / min to remove insoluble solid impurities. The supernatant was concentrated to an extract by vacuum concentration, and the solvent was recovered at the same time. Obtai...

Embodiment 3

[0038] 500 g of the raw material was frozen and pulverized until all passed through an 80-mesh sieve. According to the liquid-to-material ratio of 20:1 (mL:g), adding a concentration of 75% edible ethanol aqueous solution is prepared, and the prepared feed liquid is pumped into the countercurrent ultrasonic extraction chamber with a feed rate of 200mL / min by a peristaltic pump. Protect and stir with 50ml / min helium, process with ultrasonic frequency 33kHz, ultrasonic power 1000W, ultrasonic working / intermittent time ratio 2:5, initial temperature of feed liquid 30°C, ultrasonic time 20min. During the process of ultrasonic treatment, the material liquid is continuously stirred to ensure uniform ultrasonic treatment. After the extraction, the extract was refrigerated and centrifuged at 10000r / min to remove insoluble solid impurities. The supernatant was concentrated to an extract by vacuum concentration, and the solvent was recovered at the same time. 259g of extract was obtai...

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Abstract

The invention discloses a countercurrent ultrasonic preparation method of propolis extract, belonging to the field of development and utilization of propolis active ingredients. The preparation method comprises the technological processes of smashing raw materials, adding solvent for blending, performing ultrasonic extraction, performing solid-liquid separation, drying, recycling the solvent and the like, has the advantages that the active ingredients in propolis can be circularly extracted by utilizing countercurrent energy-gathered ultrasonic wave, meanwhile easy-to-oxidize ingredients are prevented from losing in the ultrasonic extraction by helium protection, compared with the existing method, the extraction time is shortened by 48 to 99.8 percent, the extraction rate of soluble substances is improved by 37.2 to 172 percent, and the flavonoid content of extract is improved by 46 to 279 percent.

Description

technical field [0001] The invention relates to the field of development and utilization of propolis active ingredients, in particular to a countercurrent ultrasonic preparation method of propolis extract. Background technique [0002] Propolis is a mixture formed by honeybees collecting young plant shoots or juices and mixing them with the secretions of their own palate glands. Contains many organic acids, phenols, lipids, higher alcohols, terpenes, flavonoids and other physiologically active ingredients. Its different forms of extracts have various physiological effects such as antibacterial, promoting wound healing, anesthesia, anti-oxidation, and regulating blood sugar. Because the output of propolis is very small, and the global annual output is less than 800 tons, it is a bottleneck problem in the current development of propolis to study the extraction technology of active components of propolis, improve the utilization rate of propolis as much as possible, and save t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K35/644
CPCA61K35/644
Inventor 丁青芝马海乐廖敏骆琳张庭任晓峰马士巧
Owner JIANGSU UNIV