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Breeding method for somaclonal variation of caladium bicolor

A technology of taro mosaic and clones, which is applied in the field of breeding of somatic clonal variation of taro mosaic, can solve the problems of unstable genetic traits, slow process, long breeding cycle, etc., and achieve shorter plant type and faster speed , The effect of better leaf texture

Inactive Publication Date: 2016-05-11
ENVIRONMENTAL HORTICULTURE RES INST OF GUANGDONG ACADEMY OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Clonal variation selection is the most important breeding method for cultivating new varieties of the genus Synapsis. However, the selection of individual mutants of Synapsis mainly adopts the method of artificial selection, and some of the obtained mutant plants still have the problem of genetic instability.
[0004] At present, the breeding of taro mosaic mainly adopts the conventional hybrid breeding method, which has problems such as long breeding cycle and slow process, and the research on its somaclonal variation breeding has just started, and there is no relevant literature report in China

Method used

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  • Breeding method for somaclonal variation of caladium bicolor
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  • Breeding method for somaclonal variation of caladium bicolor

Examples

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Effect test

Embodiment 1

[0051] A breeding method for obtaining a new variety of C2-0 mutant single plant by using the taro cultivar 'Rose Bud' as the parent material.

[0052] The method proceeds as follows:

[0053] (1) Preparation of medium:

[0054] The culture medium consists of the following three parts:

[0055] a) Induction medium: on the basis of MS medium, add 0.5-2mg / L 6-BA, 0.1-2mg / L 2,4-D, 20-40g / L sucrose and 6-8g / L karaage Glue, pH5.6~6.0;

[0056] b) Differentiation medium: on the basis of MS medium, add 1-3mg / L 6-BA, 0.1-1mg / L NAA, 20-40g / L sucrose and 6-8g / L carrageenan, pH5. 6~6.0;

[0057] c) Medium for rooting and strong seedlings: on the basis of MS medium, add 0.1-1 mg / L NAA, 20-40 g / L sucrose and 6-8 g / L carrageenan, pH 5.6-6.0;

[0058] (2) Selection and disinfection of explants: select the young leaves of the parent 'rosebud' that have not yet unfolded as explants, soak them in 70% ethanol for 30 seconds, rinse them twice with sterile water, soak them in 0.1% mercury chlor...

Embodiment 2

[0067] A breeding method for obtaining a new variety C3-0 with variation single plant using the taro cultivar 'Fliida' as the parent material. The method proceeds as follows:

[0068] (1) Preparation of medium:

[0069] The culture medium consists of the following three parts:

[0070] a) Induction medium: on the basis of MS medium, add 0.5-2mg / L 6-BA, 0.1-1mg / L 2,4-D, 25-35g / L sucrose and 6-8g / L karaage Glue, pH5.8~6.0;

[0071] b) Differentiation medium: on the basis of MS medium, add 1-2mg / L 6-BA, 0.1-0.5mg / L NAA, 25-35g / L sucrose and 6-8g / L carrageenan, pH5 .8~6.0;

[0072] c) Medium for rooting and strong seedlings: on the basis of MS medium, add 0.1-0.5 mg / L NAA, 25-35 g / L sucrose and 6-8 g / L carrageenan, pH 5.8-6.0;

[0073] (2) Selection and disinfection of explants: select the unexpanded young leaves of the parent 'Frieda' as explants, soak them in 70% ethanol for 30 seconds, wash them twice with sterile water, and soak them in 0.1% mercuric chloride for 8~ 10mi...

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Abstract

The invention discloses a breeding method for somaclonal variation of caladium bicolor, and belongs to the technical field of genetic breeding of flowers. The method comprises the steps that a tissue culture rapid propagation system of a parent is built in a callus induction way; variant plants with special characters are screened out of offspring groups and numbered, the content of relative nuclear DNA of the variant plants is detected, and when the peak value position of the variant plants is 1.6 to 2.2 times that of the parent, the corresponding plants are determined as preferable plants of the caladium bicolor; a clonal offspring group of the preferable plants is built, multi-year multi-point experimental research is carried out on the main characters of the offspring group, the specificity, consistency and stability of the preferable plant offspring group are verified, and finally the preferable plants are determined as a new variety of the caladium bicolor. According to the method, good variant plants of the caladium bicolor can be effectively obtained, the variant plants can be stabilized fast, an operating method is simple and convenient, the breeding cycle is shortened, and breeding efficiency is improved.

Description

technical field [0001] The invention belongs to the technical field of flower genetics and breeding, and in particular relates to a breeding method for somatic clone variation of taro mosaic. Background technique [0002] Mosaic taro ( caladium×hortulanum ) is a perennial herb of the family Araceae, native to the tropical regions of South America. The leaf color changes are very rich, including red, pink, white, yellow, purple, etc., and the colors are bright and unique. They are deeply loved by people and are widely used in gardens in western countries. It can also be used as potted plants and cut leaves for production, and is an important color foliage plant. [0003] Somaclonal Variation (Somaclonal Variation) generally refers to the genetic variation or epigenetic variation produced in the process of plant cell, tissue and organ culture. Using this method, many variable traits with economic value can be obtained, such as disease resistance, dwarfing, stress resistance ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 刘金梅赵超艺钟荣辉于波尤毅章金辉陈香罗
Owner ENVIRONMENTAL HORTICULTURE RES INST OF GUANGDONG ACADEMY OF AGRI SCI
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