Application of a kind of tea volatile essential oil in the preparation of anti-cancer health products or anti-cancer drugs

A technology of tea volatility and anti-cancer drugs, which is applied in application, drug combination, drug delivery, etc., can solve the problem that the health care function is seldom paid attention to in processing, and achieve strong anti-cancer health care and anti-cancer effects, good application Prospect, effect of inhibiting cancer cell growth

Active Publication Date: 2019-02-12
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In terms of tea deep processing, tea water extraction to prepare instant tea and concentrated tea juice for tea beverage processing are widely used. Tea polyphenols isolated from tea, including catechins, are widely used in food additives or in the production of health food, and some are even used in In the field of medicine, such as anti-inflammatory drugs and anti-cancer research, but the volatile essential oils of tea are aroma substances, and its health function application is rarely paid attention to in processing

Method used

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  • Application of a kind of tea volatile essential oil in the preparation of anti-cancer health products or anti-cancer drugs
  • Application of a kind of tea volatile essential oil in the preparation of anti-cancer health products or anti-cancer drugs
  • Application of a kind of tea volatile essential oil in the preparation of anti-cancer health products or anti-cancer drugs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] The preparation method of embodiment 1 tealeaves volatile essential oil

[0019] (1) Weigh 25g of ground tea sample into a 1L round bottom flask, add first-grade water according to the ratio of 5-20ml of water per gram of tea raw material, connect to one end of the simultaneous distillation and extraction device, and heat with an electric heating mantle to keep a slight boil; the other end is connected to a round-bottomed flask filled with 50mL of dichloromethane, heated with a water bath, and the temperature is kept at 55°C; the extraction time is 60-180min.

[0020] (2) After the extraction, the distillate was collected and transferred to a sample bottle, and the dichloromethane was evaporated with nitrogen to obtain a tea volatile essential oil sample.

Embodiment 2

[0021] Example 2 Anti-tumor cell efficacy research of tea volatile essential oil in vitro, comprising the following steps:

[0022] (1) Prepare the Hepa1c1c7 mouse liver cancer cell suspension, get the cells in the logarithmic growth phase and in good condition, digest with 0.25% trypsin (mass percentage), and prepare 5×10 cells with the culture medium 3 cells / mL cell suspension, inoculated in 96-well culture plate, 200 μL per well, inoculated two plates. 37°C, 5% (volume percent) CO 2 Cells were cultured in the incubator for 24 h.

[0023] (2) According to a certain dose, the volatile essential oil of tea is prepared into a certain concentration solution containing the sample with a-MEM culture medium (volume percentage) containing 10% fetal bovine serum, and 150 μL of the solution is added to each well, and mouse liver cancer cells will not be added. The control group was used as the blank control group, and the same amount of fresh culture medium was added to the control ...

Embodiment 3

[0033] Anticancer activity evaluation of embodiment 3 tealeaves essential oil (1)

[0034] Black tea, green tea, black tea, white tea, green tea, and yellow tea are represented by 6 major teas, using the method in Example 1 to obtain the tea volatile essential oil, and using the method described in Example 2, to obtain the induced quinone by linear fitting curve Specific concentrations of reductases produced doublings.

[0035] Table 1. Concentration (μg / ml) of quinone reductase production doubled by tea essential oils induced by six major teas

[0036] tea

Test tea representative

QR Multiplier Concentration

black tea

Yunnan big leaf black tea

5.32

green tea

Yunnan big leaf green tea

12.32

black tea

Yunnan large-leaf Pu-erh tea

3.03

white tea

Fuding white tea

8.96

green tea

Phoenix Single Cong (Milan Fragrance)

23.08

yellow tea

Hunan Junshan Silver Needle

7.39

[0037] Th...

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Abstract

The invention belongs to the field of tea processing and discloses application of tea volatile essential oil to preparation of an anti-cancer health care product or an anti-cancer drug. The anti-cancer health care product or the anti-cancer drug refers to the health care product or the drug used for preventing or treating the cancer with a quinone reductase inductive effect. The tea volatile essential oil is prepared from tea leaves being a raw material through a simultaneous distillation-extraction method. The obtained tea volatile essential oil has a pleasant smell, can soothe the anxious emotion and has a certain anti-cancer effect; when the concentration of the volatile essential oil extracted from 6 types of tea including black tea, green tea, oolong tea, yellow tea, white tea and dark green tea is in the range of 3-30 micrograms / ml, quinone reductase can be induced to multiply, that is, the anti-cancer effect is achieved. Research results indicate that the tea volatile essential oil achieves the anti-cancer effect by inducing the quinone reductase, is a potential anti-cancer active substance and can be used for prepare the anti-cancer health care product or the anti-cancer drug.

Description

technical field [0001] The invention belongs to the technical field of deep processing of tea, and in particular relates to the application of volatile essential oil of tea in preparing anti-cancer health products or anti-cancer drugs. Background technique [0002] China has a long history of tea drinking and rich tea tree resources, which provide unique conditions for tea drinkers. Tea is one of the most popular health beverages. Tea contains tea protein, tea polyphenols, tea polysaccharides, caffeine, tea saponins and other functional ingredients, which have anti-tumor, hypoglycemic, blood lipid, blood pressure, and antioxidant It has broad development prospects as health food and medicine. [0003] There are many types of aroma substances in tea, but the content is very small, generally accounting for 0.01-0.05% of the dry matter content of tea, green tea is about 0.005-0.02%, black tea is about 0.01-0.03%, and oolong tea is about 0.05%. Tea aroma substances can be divi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23L33/00A61K36/82A61P35/00
CPCA23V2002/00A61K9/007A61K36/82A61K2236/35A23V2200/308A23V2250/214
Inventor 陈忠正李斌张媛媛林晓蓉燕妮高雄李晓飞李晓玲
Owner SOUTH CHINA AGRI UNIV
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