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Tissue cell culture device

A culture device and tissue cell technology, applied in the field of biological culture devices, can solve the problems of different cell culture methods

Inactive Publication Date: 2016-05-11
ANHUI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Cell culture has become an indispensable and important part of the current scientific research process, but different cell culture methods are different, common cells are divided into suspension and adherent culture, and a special kind of cells are tissue cells, which need both oxygen and oxygen during the culture process. Culture fluid is needed, and the tissue cells need to be in contact with both the culture fluid and the air during the culture process, but there is still no better method and device to solve this problem in the existing culture equipment

Method used

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specific Embodiment

[0031] The tray 11 has a diameter of 40cm, and the front of the tray 11 has grooves 16a, 16b with a diameter of 6cm and 10cm corresponding to each other. The back side is provided with two annular chute 11a, 11b, and the radius of two annular chute 11a, 11b is 20cm, 16cm respectively, and tray 11 center is flat hole 111, and depth is 3cm, protrudes disc front, and purpose is to prevent tray 11 from contacting with The column 13 is disengaged and when the turntable 12 rotates, the holding tray does not rotate thereupon.

[0032] On the turntable 12, the heights of the two bumps 15a, 15b are 1.5cm, 0.6cm, because the distances from the two bumps 15a, 15b to the center of the turntable are respectively 20cm, 16cm, and the amount of liquid in the culture dish 20 is generally 0.5cm. Height, the distance between the upper and lower plates is 1 cm, so the height difference can make the bump 15a lift up one end of the tray 11, and the bump 15b can support the tray to prevent excessive...

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Abstract

The invention belongs to a biological culture device, and in particular, relates to a tissue cell culture device comprising a tray; the tray is provided with fixing units for fixing culture dishes; the tray is movably arranged on a base; the base is provided with a circumferential limit unit for preventing the tray from circumferentially rotating; the bottom of the tray is horizontally provided with a rotary disk; the rotary disk is driven by a motor; the disk surface of the rotary disk is provided with two convex blocks which have smooth surfaces, wherein one of the two convex blocks is high and the other of the two convex blocks is low; the top parts of the two convex blocks make contact with the bottom surface of the tray and form sliding fit. In the rotation process, the upper convex block of the rotary disk can make the culture dishes on the tray periodically fluctuate and flip, a culture liquid in the culture dishes flows back and forth, and tissue cells at the bottoms of the culture dishes are intermittently exposed in air to meet the growth demand that the tissue cells need not only oxygen gas but also the culture liquid.

Description

technical field [0001] The invention belongs to a biological culture device, in particular to a tissue cell culture device. Background technique [0002] Cell culture has become an indispensable and important part of the current scientific research process, but different cell culture methods are different, common cells are divided into suspension and adherent culture, and a special kind of cells are tissue cells, which need both oxygen and oxygen during the culture process. Culture fluid is needed, and the tissue cells need to be in contact with both culture fluid and air during the culture process, but there is still no better method and device to solve this problem in the existing culture equipment. Contents of the invention [0003] The object of the present invention is to provide a tissue cell culture device capable of contacting tissue cells with both culture solution and air. [0004] In order to achieve the above object, the present invention provides the followin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/00C12M1/22
CPCC12M21/08C12M23/10C12M23/48C12M27/16
Inventor 曹鸿国牛梦涵贾镛章孝荣
Owner ANHUI AGRICULTURAL UNIVERSITY
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