Brevundimonas vesicularis s3 and its application
A technology of brevundimonas and vesicles, applied in the field of microorganisms, to achieve the effect of less restricted conditions, less environmental interference factors, and avoid competition
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Embodiment 1
[0024] Pseudomonas brevundimonas naejangsanensis S3; preserved in the General Microbiology Center of China Committee for the Collection of Microbial Cultures, the preservation address is No. 3, No. 1 Beichen West Road, Chaoyang District, Beijing, Institute of Microbiology, Chinese Academy of Sciences, preservation number It is CGMCC NO.11072, and the deposit date is July 10, 2015.
[0025] Brevundimonas naejangsanensis S3 can grow in an environment with a pH value of 6-8; the suitable growth temperature is 25°C-35°C.
[0026] Brevundimonas naejangsanensis S3 was cultured in 50 mL of beef extract peptone liquid medium, and gentamicin (G), neomycin (N), streptomycin (S), and ampicillin were added (A), penicillin sodium (P), cephalosporin (C), and sulfathiazole (STZ) concentrations are 32mg / L, 32mg / L, 128mg / L, 64mg / L, 64mg / L, 64mg / L, 64mg / L, Cu 2+ , Zn 2+ Concentrations are 200mg / L, 400mg / L, respectively, 120r / min in a shaking incubator, 30 ℃ dark culture after 3 days, the de...
Embodiment 2
[0028] In this embodiment, Brevundimonas naejangsanensis S3 is isolated and obtained according to the following steps.
[0029] Soil samples were collected in the mulberry field of Jiangsu University of Science and Technology. Take samples according to the 5-point sampling method, then mix all the collected soil, air-dry, and pass through a 100-mesh sieve to obtain soil samples. Then treated with 300mg / kg copper sulfate and 1000mg / kg zinc sulfate for 84 days. Weigh 1g of the soil sample and add it to 99ml of sterile saline, shake it on a shaker at 120r / min at 25°C for 2h to make soil 10 -4 The diluent is ready for use. Take the diluted solution and spread it on the plate, and incubate at 30°C for 2-4 days. Described plate culture medium is basic inorganic salt (MSM) medium (g / L): K 2 HPO 4 5.8, KH 2 PO 4 4.5, (NH 4 ) 2 SO 4 2.0, MgCl 2 0.16, CaCl 2 0.02, Na 2 MoO 4 2H 2 O0.0024, FeCl 3 0.0018, MnCl 2 2H 2 O 0.0015, 18g of agar, pH = 7.0, sterilized at 121°C f...
Embodiment 3
[0031] Brevundimonasnaejangsanensis S3, an antibiotic-degrading bacterium resistant to heavy metals in this embodiment, can be used to degrade antibiotics in heavy metal-polluted environments.
[0032] Brevundimonasnaejangsanensis S3 was added to the environment polluted by heavy metals and antibiotics, and the environment temperature was 25°C-35°C, and the environment pH value was 6-8. Brevundimonas naejangsanensis S3 in this embodiment can be used for remediation of contaminated soil or water.
[0033] Select gentamicin, neomycin, streptomycin, ampicillin, penicillin sodium, cephalosporin and sulfathiazole so that the final concentration reaches 32mg / L, 32mg / L, 128mg / L, 64mg / L, 64mg / L, respectively. L, 64mg / L and 64mg / L, Cu 2+ , Zn 2+ Concentrations of 200mg / L, 400mg / L compound polluted soil 100g, the vesicular Brevundimonas naejangsanensis (Brevundimonas naejangsanensis) S3 cultured in the beef extract peptone liquid medium in the early stage of stationary phase was spray...
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