Method for carrying out enzyme-linked immunosorbent assay (ELISA) reactions by embedding suction head

Abstract

The invention relates to a method for carrying out ELISA reactions by embedding a suction head. The method comprises the following steps: (1) subjecting a suction head to be embedded to a surface treatment; (2) washing the suction head: inserting a pipettor into the suction head, and washing the suction head for three times by washing liquid; (3) embedding the suction head; (4) after embedding, washing the suction head; (5) adding a confining liquid; (6) washing the suction head after the confining liquid is added; (7) carrying out ELISA experiment after embedding. According to the ELISA experiment, the habit that the ELISA plate is embedded is changed, the suction head is embedded instead, the limitation of ELISA plate is broken through, the ELISA plate can be used flexibly, the liquid is drained by the strong dispelling function of pipettor, the operation is very convenient, the liquid is absorbed, at the same time, the antibody and antigen are combined, the time is saved, and the efficiency is improved.

Description

technical field [0001] The invention belongs to the technical field of medical detection, in particular to a method for performing enzyme-linked immunological reaction by using tip coating. Background technique [0002] Enzyme-linked immunosorbent assay, referred to as enzyme-linked immunosorbent assay, or ELISA method. Its center is to bind the antibody to the enzyme complex and then detect it by color development. The basic principles are: ① Make the antigen or antibody bind to the surface of a certain solid phase carrier and maintain its immune activity. ②The antigen or antibody is connected with an enzyme to form an enzyme-labeled antigen or antibody, which not only retains its immune activity, but also retains the activity of the enzyme. During the determination, the test specimen (to determine the antibody or antigen in it) and the enzyme-labeled antigen or antibody react with the antigen or antibody on the surface of the solid phase carrier in different steps. The ...

AI Technical Summary

Problems solved by technology

Although there are already many automatic plate washing machines and plate shakers, this washing method has irreparable shortcomings. First, it is cumbersome to operate and has many processes. Most of the design is a flat bottom design. When the liquid is washed and drained in the microplate, the liquid will remain at the bottom due to tension. This can only be achieved by increasing the number of cycles of washing and shaking the plate and increasing the soaking time. , Increase the flushing pressure and flushing time to make up for it. It is difficult to completely clean and shake the plate when washing and shaking the plate. This is not only time-consuming and labor-intensive, but also very likely to cause inaccurate experiments.

However, since the experiment needs to be carried out with the aid of a microplate reader, it cannot be separated from the microplate plate