Quick deep-layer liquid state fermentation method for producing grifola frondosa bacterial powder

Abstract

The invention discloses a quick deep-layer liquid state fermentation method for producing grifola frondosa bacterial powder. According to the method, the semi-continuous fermentation process is adopted to treat grifola frondosa strains to obtain fermentation liquor rich in abundant mycelia fast, and then the fermentation liquor is treated and separated through a micro-filtration membrane to obtain mycelia of grifola frondosa. The traditional intermittent grifola frondosa batch fermentation process is changed, and the mycelia are separated in the mode of efficient ceramic membrane micro-filtration instead of the separation mode of a centrifugal machine. The semi-continuous fermentation process overcomes the defects that the number of process links of traditional batch fermentation is large, middle clearance time is long, and equipment efficiency is low. The efficient fermentation liquor filtered through the ceramic membrane is adopted, the mycelia entering filter liquor can be reduced, the yield of the mycelia can be increased, COD of the filter liquor is reduced, the sewage handling capacity is reduced, damage to cells is avoided, and it is ensured that effective ingredients in the cells are recovered completely. Compared with the traditional technology, the method has remarkable economic benefits, social benefits and environment-friendly benefits.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a method for rapidly producing fungal mycelia by deep liquid fermentation technology, more precisely, a method for rapidly producing grifola frondosa fungus powder by deep liquid fermentation. Background technique [0002] Microbial fermentation can be divided into batch, fed-batch, semi-continuous, continuous and other modes. Batch fermentation consumes a lot of manpower and material resources. Each batch of fermentation needs to be loaded, sterilized, inoculated, discharged, and cleaned. The process is cumbersome, the fermentation cycle is long, and the production efficiency is low; Fed-batch fermentation Although the lack of nutrients or precursors can be supplemented by feeding, the product synthesis will inevitably be blocked due to the continuous accumulation of harmful metabolites; the production intensity of continuous fermentation is greatly improved c...

AI Technical Summary

Problems solved by technology

[0006] The traditional Grifola frondosa liquid fermentation process is mostly an intermittent batch fermentation process, but the batch fermentation process consumes a lot of manpower and material resources, and each batch of fermentation needs to be loaded, sterilized, inoculated, discharged, and cleaned. , the process is cumbersome, the non-fermentation cycle is long, and the production efficiency is low; while the subsequent separation process is traditionally separated by a centrifuge, this method is subject to the influence of the separation factor of the centrifuge, and the mycelium is damaged by shear force during the separation process , some active ingredients are lost into the filtrate, which leads to a decrease in product quality and yield. The separated filtrate is turbid and contains more broken mycelia, resulting in a high COD of the discharged filtrate. If the filtrate meets the sewage discharge standard, it needs to go through complicated procedures Sewage treatment, the workload of the sewage treatment system is too large, which brings a serious economic burden to the enterprise

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