Quick deep-layer liquid state fermentation method for producing xylariasp bacterial powder

A technology of submerged liquid fermentation and Wuling ginseng bacteria powder, which is applied in the field of microorganisms, can solve the problems of heavy workload in the sewage treatment system, decline in product quality and yield, and high consumption of manpower and material resources, so as to improve equipment utilization and filtrate Clarification and easy maintenance of the device

Inactive Publication Date: 2016-05-18
JIANGSU SHENHUA PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The traditional liquid fermentation process of Wuling ginseng is mostly an intermittent batch fermentation process, but the batch fermentation process consumes a lot of manpower and material resources. Each batch of fermentation needs to be loaded, sterilized, inoculated, discharged, and cleaned. , the process is cumbersome, the non-fermentation cycle is long, and the production efficiency is low; while the subsequent separation process is traditionally separated by a centrifuge, this method is subject to the influence of the separation facto

Method used

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  • Quick deep-layer liquid state fermentation method for producing xylariasp bacterial powder
  • Quick deep-layer liquid state fermentation method for producing xylariasp bacterial powder
  • Quick deep-layer liquid state fermentation method for producing xylariasp bacterial powder

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Embodiment 1

[0028] 1) Wulingshen strain (Xylarianigripes (Kl.) Sacc.) was provided and identified by the Institute of Microbiology, Chinese Academy of Sciences, with a deposit number of CGMCCNO.1038. Seed the cultured Wulingshen strain seed liquid into the seed medium, the inoculum size is 10%, and the seed medium is composed of glucose 2%, peptone 0.5%, soybean cake powder 1.5%, potassium dihydrogen phosphate 0.3%, heptahydrate Magnesium sulfate 0.2%, zinc sulfate 0.25%, soybean oil 0.02%; adjust the pH value to 5.5. The culture conditions are: the ventilation ratio is 1:0.5vvm, the tank pressure is 0.03Mpa, the culture temperature is 26-28°C, and the seed liquid can be obtained after culturing for about 4 days. Inoculate the cultivated seed liquid into 50L fermentation medium after sterilization, the inoculum size is 15%, and the fermentation medium is composed of 2% glucose, 0.6% peptone, 1.5% soybean cake powder, 0.3% potassium dihydrogen phosphate, magnesium sulfate heptahydrate 0.3...

Embodiment 2

[0036] 1) Wulingshen strain (Xylarianigripes (Kl.) Sacc.) was provided and identified by the Institute of Microbiology, Chinese Academy of Sciences, with a deposit number of CGMCCNO.1038. Seed the cultured Wulingshen strain seed liquid into the seed medium, the inoculum size is 10%, and the seed medium is composed of 2% glucose, 0.6% peptone, 1.8% soybean cake powder, 0.3% potassium dihydrogen phosphate, heptahydrate Magnesium sulfate 0.3%, zinc sulfate 0.25%, soybean oil 0.02%; adjust the pH value to 5.5. The culture conditions are: the ventilation ratio is 1:0.5vvm, the tank pressure is 0.03Mpa, the culture temperature is 26-28°C, and the seed liquid can be obtained after culturing for about 4 days. Inoculate the cultivated seed liquid into 100L fermentation medium after sterilization, the inoculum size is 15%, and the fermentation medium is composed of 2% glucose, 0.8% peptone, 2.0% soybean cake powder, 0.3% potassium dihydrogen phosphate, magnesium sulfate heptahydrate 0....

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Abstract

The invention discloses a quick deep-layer liquid state fermentation method for producing xylariasp bacterial powder. According to the method, the semi-continuous fermentation process is adopted to treat xylariasp strains to obtain fermentation liquor rich in abundant mycelia fast, and then the fermentation liquor is treated and separated through a micro-filtration membrane to obtain mycelia of xylariasp. The traditional intermittent xylariasp batch fermentation process is changed, and the mycelia are separated in the mode of efficient ceramic membrane micro-filtration instead of the separation mode of a centrifugal machine. The semi-continuous fermentation process overcomes the defects that the number of process links of traditional batch fermentation is large, middle clearance time is long, and equipment efficiency is low. The efficient fermentation liquor filtered through the ceramic membrane is adopted, the mycelia entering filter liquor can be reduced, the yield of the mycelia can be increased, COD of the filter liquor is reduced, the sewage handling capacity is reduced, damage to cells is avoided, and it is ensured that effective ingredients in the cells are recovered completely. Compared with the traditional technology, the method has remarkable economic benefits, social benefits and environment-friendly benefits.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to a method for rapidly producing fungal mycelium by deep liquid fermentation technology, more precisely, a method for rapidly producing Wulingshen bacteria powder by deep liquid fermentation. Background technique [0002] Microbial fermentation can be divided into batch, fed-batch, semi-continuous, continuous and other modes. Batch fermentation consumes a lot of manpower and material resources. Each batch of fermentation needs to be loaded, sterilized, inoculated, discharged, and cleaned. The process is cumbersome, the fermentation cycle is long, and the production efficiency is low; Fed-batch fermentation Although the lack of nutrients or precursors can be supplemented by feeding, the product synthesis will inevitably be blocked due to the continuous accumulation of harmful metabolites; the production intensity of continuous fermentation is greatly improved compared w...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12N1/02C12R1/645
CPCC12N1/14C12N1/02
Inventor 王文风徐国华徐玲杨亚威张芙蓉孙妮娜
Owner JIANGSU SHENHUA PHARMA
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