A method for detecting honeysuckle and mountain silver-flower medicinal materials by rapid high-resolution liquid chromatography

A technology of liquid chromatography and detection method, which is applied in the detection field of Chinese medicinal materials honeysuckle and mountain silver flower, can solve the problems of differentiation and long analysis time, and achieve the effect of simple operation, good reproducibility and reliable method

Active Publication Date: 2018-01-26
HUAZHONG UNIV OF SCI & TECH
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these methods not only take a long time to analyze, but also need to use a large amount of organic solvent as mobile phase
At present, there is no more convenient method than LC to distinguish mountain silver flower from honeysuckle, especially the RRLC method

Method used

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  • A method for detecting honeysuckle and mountain silver-flower medicinal materials by rapid high-resolution liquid chromatography
  • A method for detecting honeysuckle and mountain silver-flower medicinal materials by rapid high-resolution liquid chromatography
  • A method for detecting honeysuckle and mountain silver-flower medicinal materials by rapid high-resolution liquid chromatography

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Experimental program
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Effect test

Embodiment 1

[0089] Identification and detection of embodiment 1 honeysuckle and mountain silver flower

[0090] 1. Prepare the sample solution to be tested: take 5 g of the sample to be tested, grind it into a fine powder (through a No. 2 sieve) with a mortar, take 0.10 g of the fine powder, weigh it accurately, then add 10 ml of 70% methanol aqueous solution, and heat at 37 ° C Sonicate for 15min. After sonication, it was left to cool to room temperature, and adjusted to 10 ml with 70% methanol aqueous solution. Before sample injection, the sample solution to be tested was filtered with a 0.20 μm filter membrane, 1 ul sample was taken for injection, and the chromatogram was recorded.

[0091] 2. Chromatographic conditions: the chromatographic column is filled with octadecylsilane bonded silica gel (Luna C18-HST (2) column (2.5μm, 100mm×3.0mm)); 0.1% formic acid aqueous solution is used as mobile phase A, and The acetonitrile solution containing 0.1% formic acid is the mobile phase B, t...

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Abstract

The invention provides a method for detecting honeysuckle flower and lonicerae flos medicinal materials through rapid resolution liquid chromatography (RRLC). Honeysuckle flowers have the remarkable biological activities of resisting bacteria, inflammations, viruses and the like, and can be widely added into related products such as compound medicines, food and beverages. Due to the fact that the price spread of honeysuckle flowers and lonicerae flos is large, the phenomenon that lonicerae flos is adopted as the main substitutes of honeysuckle flowers for honeysuckle flower product adulterating exists on the market. The method can simultaneously analyze the compounds of chlorogenic acid, galuteolin, macranthoidin B and dipsacoside B contained in honeysuckle flowers and lonicerae flos within 8 min, can be used for identifying honeysuckle flower and lonicerae flos medicinal materials so as to monitor the mass of the honeysuckle flower raw materials and products, and has the good precision, repeatability and accuracy.

Description

technical field [0001] The invention belongs to the field of medicine and relates to a detection method for Chinese medicinal materials, in particular to a detection method for Chinese medicinal materials honeysuckle and mountain silver flower. Background technique [0002] Honeysuckle is the dry flower bud or the first flower of Lonicera japonica Thunb. in the family Caprifoliaceae, native to eastern Asia, including China, Japan, and Korea. Honeysuckle has the functions of clearing heat and detoxifying, dispelling wind-heat, antibacterial and anti-inflammation, etc. It is widely used to treat carbuncle, acute dysentery, pharyngitis and upper respiratory system diseases caused by viral or bacterial infection. In addition, honeysuckle also has pharmacological effects such as antibacterial, anti-inflammatory and antiviral (such as: H1N1 influenza virus). Shuanghuanglian Oral Liquid is a traditional compound Chinese medicine. Honeysuckle is one of its main raw materials. It is...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02
CPCG01N30/02G01N2030/027
Inventor 尹春萍马元春
Owner HUAZHONG UNIV OF SCI & TECH
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