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A kind of alkaloid compound and its preparation method and use

A technology of alkaloids and compounds, applied in the field of natural medicinal chemistry, can solve the problem that the breeding of new disease-resistant varieties is indispensable

Active Publication Date: 2021-01-01
INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Therefore, the comprehensive control technology of bacteria, fertilizer and medicine and the breeding of new disease-resistant varieties are indispensable.

Method used

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  • A kind of alkaloid compound and its preparation method and use
  • A kind of alkaloid compound and its preparation method and use
  • A kind of alkaloid compound and its preparation method and use

Examples

Experimental program
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Effect test

Embodiment 1

[0034]Fresh onion lotus bulbs, chopped, dried to get 40 kg, extracted with 95% ethanol cold soaking 3 times, 10 days each time, filtered to obtain the cold soaking extract, and the filter residue was extracted 4 times with 95% ethanol hot reflux, 2h / time , Combine the extracts from cold soaking and hot extracting, concentrate under reduced pressure to obtain extract, add water to dissolve. The aqueous solution was passed through a macroporous resin D101 column and eluted with different concentrations of ethanol, and 147 g of extract was obtained from the 30% ethanol part. 147 g of the obtained extract was applied to a silica gel column, eluted with chloroform-methanol-water system for segmental and repeated column chromatography to obtain compound Yzec-3, the purity of which was detected by HPLC-MS was greater than 98%. After the structure was analyzed by mass spectrometry and nuclear magnetic resonance, it was named dihydronarcissine clasine-4- O - α -L-arabinoside (dihydro...

Embodiment 2

[0042] Lycoris bulbs were extracted 3 times with 80% ethanol hot reflux, 2h / time, filtered and combined extracts, concentrated to a small volume, dispersed with water, and subjected to macroporous resin AB-8 column chromatography, collecting 30% alcohol eluted parts, and then with reverse phase C 18 Silica gel column chromatography, eluted with 60% ethanol, repeated 3 times, obtained. The purity was greater than 90% as detected by HPLC.

Embodiment 3

[0044] Lycoris bulbs were extracted 3 times with 75% ethanol hot reflux, 2h / time, filtered and combined extracts, concentrated to a small volume, dispersed with water, and then subjected to macroporous resin D101 column chromatography, collecting 30% ethanol eluted parts, and then used Silica gel column chromatography, elution with chloroform-methanol-water system, and reverse phase C 18 Silica gel column chromatography, eluted with 60% ethanol. The purity is greater than 85% as detected by HPLC.

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Abstract

The invention relates to the natural pharmaceutical chemistry field and the technical field of biopesticides, and discloses a new alkaloid compound, a preparation method thereof and application in preparing antimicrobial pesticides. The alkaloid compound has a structure as shown in a drawing of the abstract, and has a chemical name of dihydronarciclasine-4-O-alpha-L-arabinopyranoside. The preparation method is characterized by comprising the following steps: by taking Amaryllidaceae plants as raw materials, extracting by methyl alcohol, ethyl alcohol or water-containing alcohol, filtering, concentrating to a small volume, dispersing by using water, then performing macroporous resin column chromatography, collecting at a part eluted by 30 percent of the ethyl alcohol, and then performing column chromatography repeatedly with column chromatography filler being silica gel or reverse phase silica gel, thus obtaining a new alkaloid. The Amaryllidaceae plants are Zephyranthescandida, Lycorisradiata, Lycorislongituba and Lycorisaurea. As a natural plant bactericide, the compound has remarkable effects of resisting Magnaporthe orythae and Verticillium dahliae, can be singly used, and also can be compounded with other bactericides to be prepared into a compounded bactericidal pesticide.

Description

technical field [0001] The invention relates to the fields of natural medicinal chemistry and biopesticide technology, and discloses a new alkaloid compound, a preparation method thereof and an application in preparing antibacterial pesticides. Background technique [0002] In the 21st century, with the enhancement of people's awareness of environmental protection and concern for food safety, people pay more and more attention to the negative effects of chemical pesticides. The development of biological pesticides has become an important trend to ensure human health and sustainable agricultural development. Plants are rich in secondary metabolites, and some secondary metabolites have become research hotspots in recent years because of their certain functions in preventing and controlling diseases and insect pests. The types of plant secondary metabolites include alkaloids, phenols, terpenes, flavonoids, essential oils, photoactivated toxins, etc. Among them, alkaloids are t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H17/00C07H1/08A01N43/90A01P3/00
CPCA01N43/90C07H1/08C07H17/00
Inventor 印敏徐曙杨简赛冯煦王鸣单宇管福琴王奇志王碧刘飞陈雨赵兴增
Owner INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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