A western blot experimenter

Abstract

The invention discloses a Western Blot laboratory instrument. The Western Blot laboratory instrument comprises a refrigeration system, a reaction box swinging system, an automatic reagent filling and recovering system and a control system, wherein the automatic reagent filling and reclaiming system is connected with a reaction box and provided with a reagent storage and recovery device; the reaction box is arranged in a reaction box groove; the reaction box swinging system is connected with the reaction box groove; the refrigeration system is used for cooling down the reaction box and the reagent storage and recovery device; the control system is connected with the refrigeration system, the reaction box swinging system, the automatic reagent filling and recovering system and the reaction box. The Western Blot laboratory instrument has the advantages that the reaction efficiency can be improved, reagent filling and reclaiming can be precisely controlled, reagent cross contamination in different experiments is avoided and the experimental reliability and precision are improved.

Description

technical field [0001] The invention relates to the field of laboratory equipment, in particular to a Western Blot experimental instrument. Background technique [0002] Western Blot experiment, that is, Western blot experiment, is an experimental method commonly used in molecular biology, biochemistry and immunogenetics. Its basic principle is to conduct gel electrophoresis-treated cells or biological tissue samples through specific antibodies Staining, a method of obtaining information about the expression of a specific protein in the analyzed cells or tissues by analyzing the position and depth of staining. The method can be divided into three stages: electrophoresis, transfer, and enzyme immunoassay. The protein samples separated by PAGE (polyacrylamide gel electrophoresis) are transferred to a solid phase support (such as nitrocellulose membrane). Adsorbs proteins in the form of non-covalent bonds, and can keep the type of polypeptide separated by electrophoresis and i...

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Problems solved by technology

[0003] At present, the Western Blot experiment process is manually operated step by step. During the entire experiment process, an automated processing flow cannot be formed. The staff need to take care of the progress of the experiment process, which takes up a lot of valuable time of the staff; and the cleaning process needs to be done manually. , the process is cumbersome and takes a long time, which increases the possibility of staff being infected by the virus; in addition, the general reagent filling is directly transported through the pump, causing pollution to the pump and pipeline, resulting in the replacement of reagents due to residual reagents. Affect the experimental results, and the current liquid filling equipment does not have the automatic filling and recovery function of reagents, resulting in the need to add a new filling device and recovery device, resulting in an increase in cost

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