A Molecular Marker-Assisted Selection Method for Transducing Excellent Temperature-sensitive Male Sterile Lines of Rapeseed

Abstract

The invention provides a molecular marker-assisted selection method for transferring an excellent rape thermo-sensitive male sterile line. The molecular marker-assisted selection method is characterized in that a CTAB method is used to extract the genome DNA of the filial generation single plant of an alternative excellent rape thermo-sensitive male sterile line, and agarose gel electrophoresis is used to detect the quality of the DNA sample; an interlocking SSR marker is used to perform PCR amplification on the genome DNA of the filial generation single plant, and the forward primer sequence and reverse primer sequence of the interlocking SSR marker are respectively 5'-CTCTCCTGATCCTCTCCTTC-3' and 5'-CTTGTAGAGAACCCGAACTG-3'; 6% polyacrylamide gel is used to perform electrophoresis detection on the PCR amplification product, and the plant with the target band is selected according to the electrophoresis detection result as the breeding material of the excellent rape thermo-sensitive male sterile line.

Description

technical field [0001] The invention relates to the field of molecular breeding, in particular to a molecular marker-assisted selection method for transducing excellent temperature-sensitive sterile lines of rapeseed. Background technique [0002] Rapeseed thermosensitive male sterile lines have the advantages of free assemblage, wide recovery source, short breeding cycle, and easy breeding of strong dominant combinations. Therefore, as a new way to utilize rapeseed heterosis, it has broad application prospects. Rapeseed thermosensitive sterile line K121S belongs to Brassica napus, and the currently cultivated rape variety is Brassica napus, which belongs to different species. , it is necessary to transfer the sterile gene and fertility conversion gene of K121S to Brassica napus through interspecific hybridization, and then select an excellent sterile line through backcrossing and artificial selection. However, artificial selection in field is generally limited by seasons. ...

AI Technical Summary

Problems solved by technology

Rapeseed thermosensitive sterile line K121S belongs to Brassica napus, and the currently cultivated rape variety is Brassica napus, which belongs to different species. , it is necessary to transfer the sterile gene and fertility conversion gene of K121S to Brassica napus through interspecific hybridization, and then select an excellent sterile line through backcrossing and artificial selection. However, artificial selection in field is generally limited by seasons. And time-consuming and labor-intensive, the selection efficiency is low

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