Method for preparing cattle hair detection antibody by feature diagnosis sequence

A technology for diagnosing sequences and detecting antibodies, which is applied in chemical instruments and methods, anti-animal/human immunoglobulins, specific peptides, etc. , the effect of strong specificity and quick identification method

Inactive Publication Date: 2016-06-22
CHINA NAT SILK MUSEUM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Cow hair keratin is a hardened, insoluble protein, which makes it difficult to identify cow hair products
At present, protein detection technology such as enzyme-linked immunos...

Method used

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  • Method for preparing cattle hair detection antibody by feature diagnosis sequence

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Effect test

Embodiment 1

[0025] A method for preparing a cow hair detection antibody using a characteristic diagnostic sequence, using the following steps:

[0026] a. Synthesis of characteristic diagnostic sequence: Fmoc polypeptide solid-phase synthesis method is used to synthesize characteristic diagnostic sequence, and a cysteine ​​residue is added to the C-terminal of the characteristic diagnostic sequence for coupling reaction. The amino acid sequence of the characteristic diagnostic sequence is GLLDSEDCKLPCNPCATTNAYGK as shown in SEQ ID No. 1 in the sequence table.

[0027] b. Coupling of the characteristic diagnostic sequence and the carrier: hemocyanin is selected as the carrier protein, and the characteristic diagnostic sequence is coupled with the hemocyanin by the SPDP connection method to obtain a complete antigen.

[0028] c. Initial immunization: Dilute the complete antigen prepared in step b to 1 mg / mL with physiological saline, mix the diluted complete antigen with complete Freund's a...

Embodiment 2

[0034] A method for preparing a cow hair detection antibody using a characteristic diagnostic sequence, using the following steps:

[0035] a. Synthesis of characteristic diagnostic sequence: Fmoc polypeptide solid-phase synthesis method is used to synthesize characteristic diagnostic sequence, and a cysteine ​​residue is added to the C-terminal of the characteristic diagnostic sequence for coupling reaction. The amino acid sequence of the characteristic diagnostic sequence is GLLDSEDCKLPCNPCATTNAYGK as shown in SEQ ID No. 1 in the sequence table.

[0036] b. Coupling of the characteristic diagnostic sequence and the carrier: hemocyanin is selected as the carrier protein, and the characteristic diagnostic sequence is coupled with the hemocyanin by the SPDP connection method to obtain a complete antigen.

[0037] c. Initial immunization: Dilute the complete antigen prepared in step b to 0.8mg / mL with physiological saline, mix the diluted complete antigen with complete Freund's ad...

Embodiment 3

[0042] A method for preparing a cow hair detection antibody using a characteristic diagnostic sequence, using the following steps:

[0043] a. Synthesis of characteristic diagnostic sequence: Fmoc polypeptide solid-phase synthesis method is used to synthesize characteristic diagnostic sequence, and a cysteine ​​residue is added to the C-terminal of the characteristic diagnostic sequence for coupling reaction. The amino acid sequence of the characteristic diagnostic sequence is GLLDSEDCKLPCNPCATTNAYGK as shown in SEQ ID No. 1 in the sequence table.

[0044] b. Coupling of the characteristic diagnostic sequence and the carrier: hemocyanin is selected as the carrier protein, and the characteristic diagnostic sequence is coupled with the hemocyanin by the SPDP connection method to obtain a complete antigen.

[0045] c. Initial immunization: Dilute the complete antigen prepared in step b to 1.2 mg / mL with physiological saline, mix the diluted complete antigen with complete Freund's...

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Abstract

The invention relates to the technical field of detection of cultural relics, and discloses a method for preparing a cow hair detection antibody using a characteristic diagnostic sequence. The following steps are adopted: a. synthesis of the characteristic diagnostic sequence; b. coupling of the characteristic diagnostic sequence with a carrier; ; d. Preparation of antiserum; e. Purification of bovine hair detection antibody. The cow hair detection antibody prepared by the method of the invention has high sensitivity and high specificity.

Description

technical field [0001] The invention relates to the technical field of cultural relic detection, in particular to a method for preparing cattle hair detection antibodies by using characteristic diagnostic sequences. Background technique [0002] China has a long history, and a large number of ox hair products have been unearthed from early Chinese tombs. These ancient ox hair products contain a lot of archaeological information. [0003] Cow hair is a natural animal hair fiber. Cow hair is composed of two parts, the epidermal scale layer and the cortex layer. It is a protein fiber, in which keratin is mainly composed of 18 kinds of α-amino acids, and is connected into a long-chain helical molecule, which contains carboxyl, hydroxyl and amino groups. etc., forming hydrogen bonds and salt bridges between molecules. [0004] Usually the identification of cow hair products is mainly the identification of cow hair keratin. Cow hair keratin is a hardened, insoluble protein, whi...

Claims

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Application Information

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IPC IPC(8): C07K16/18C07K16/06
CPCC07K16/18C07K16/06C07K2317/20
Inventor 郑海玲周旸赵丰汪自强刘剑王淑娟龙博杨海亮
Owner CHINA NAT SILK MUSEUM
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