Serum-free medium for purifying myocardial cells of stem cell sources

Abstract

The invention belongs to the technical field of cell culture of biological technique, and particularly relates to a serum-free medium for purifying myocardial cells of stem cell sources. The serum-free medium comprises the following components of 0.1-5mmol / L of L-glutamine, 2-10mmol / L of insulin, 0-800mug / mL of vitamin A, 100-300mug / mL of vitamin C (L-ascorbic acid-2-phosphate), 0.1-2ml / mL of gene reconstruction human serum albumin, 0.1-5% of amino acid, 1-5% of unnecessary amino acid, 0.1-0.5% of trace elements, lactose, quercetin, bromo deoxyribonucleoside, and RPMI (Roswell park memorial institute) sugar-free medium. The serum-free medium has the advantages that the myocardial cells of a mixing system can be purified, the ratio of other non-myocardial cells in a cell mixture is gradually decreased, and finally the myocardial cells with high purity can be obtained; the serum-free medium does not contain blood, so that the injury caused by virus pollution is avoided.

Description

technical field [0001] The invention belongs to the technical field of biological cell culture, and in particular relates to a serum-free medium for purifying cardiomyocytes derived from stem cells. Background technique [0002] Cells are the basic unit of life, just like molecules are the basic unit of matter. The functions of cells determine the functions of organs and tissues, and organs and tissues constitute a living body. Cell biology is a science that studies cells, a science that studies the structure, function, characteristics and laws of cells such as metabolism and apoptosis. [0003] Cell culture technology is the most important and basic technical method in cell biology. Cell culture is to grow cells under in vitro environmental conditions. Cell culture can subculture cells and culture them in vitro to obtain the quantity and quality of cells required for scientific research. At the same time, it can achieve the goals of expanding the number of cells, screening...

AI Technical Summary

Problems solved by technology

[0006] Under the current technological conditions, in vitro cell culture, including the culture of stem cells and cardiomyocytes, will use bovine serum in most cases. However, due to the uncertainty of its composition and the risk of foreign viruses and pathogenic factors, bovine serum will not The clinical use of cells is a hidden problem

Moreover, bovine serum products vary greatly due to different batches and different manufacturers.

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