31results about How to "Guaranteed normal culture" patented technology

The invention discloses a 3D culture, passage, cryopreservation, recovery and identification method for in-vitro organoids based on small intestines of different segments of mice. The method comprisesthe following steps: (1) separating recesses of duodenum, jejunum and ileum segments of mice; (2) performing 3D culture on the recesses of the duodenum, jejunum and ileum segments of mice, and forming organoids; (3) performing passage on organoids of small intestines of mice; (4) performing cryopreservation on the organoids of small intestines of mice; (5) performing recovery on the organoids ofsmall intestines of mice; (6) preparing frozen sections of the organoids of small intestines of mice; and (7) performing immunofluorescence labeling and identification on the frozen sections of the organoids of small intestines of mice. Compared with the prior art, the method disclosed by the invention optimizes a recess extraction manner, an in-vitro culture system and a culture medium based on the recesses of the small intestines containing stem cells, and comprises the steps of complete culture, passage, cryopreservation, recovery and identification. The small intestine organoids obtained by the method are capable of performing repeated passage, and the passage organoids have character stability.

The invention relates to a tissue engineering myocardium bioreactor constructed by pouring, perfusion and pulsation combination, belonging to the technical field of tissue engineering and biological engineering manufacture. The bioreactor comprises a cell pouring generator, a perfusion module, a pulsation generator, a culture module and a control module; the cell pouring generator, the perfusion module and the pulsation generator are communicated with the culture module through pipelines; and the culture module comprises a pulsation cavity, a pulsation effect soft tube with one closed end and a perfusion cavity, and the perfusion module and the pulsation generator carry out perfusion culture and pulsation training. The invention can improve cardiomyocytes planting depth and simulate myocardium in vivo environment, and carries out culture to obtain a tissue engineering myocardium with larger thickness and autonomously beating capacity. The bioreactor is used for realizing that the tissue engineering myocardium construction carries out perfusion culture and pulsation training simultaneously or separately on cell perfusion and tissue culture stages in a cell planting stage.

The invention relates to the field of microbiological analysis and detection, and particularly discloses a method for detecting clostridium perfringens in drinking natural mineral water. The inspection method comprises the following steps: a, filtering a water sample by adopting a filter membrane, then tightly placing the filter membrane on a bottom culture medium, paving a middle culture medium on the filter membrane, carrying out anaerobic inverted culture for 24-26h at the temperature of 35-37 DEG C, and counting the number of black bacterial colonies; and b, picking 3-5 black bacterial colonies, respectively inoculating the black bacterial colonies on a liquid thioglycolate culture medium, culturing for 18-24 hours at the temperature of 35-37 DEG C, and taking the black bacterial colonies to carry out a confirmation test. By means of the detection method, the black characteristic of the cultured bacterial colony is obvious, identification is easy, and the accuracy of the detection result is high.

The invention discloses an edible mushroom factory fresh air pre-processing system with high air cleanliness. The edible mushroom factory fresh air pre-processing system comprises a house, a passage arranged in the middle of the house, mushroom growing rooms arranged on the two sides of the passage inside the house, air conditioner internal machines arranged at the upper ends of the mushroom growing rooms, and exhaust fans arranged at the lower ends of the mushroom growing rooms. An air tight chamber is arranged at the upper end of the passage, one side of the air tight chamber is provided with an air inlet, an efficient filtering net is arranged at the air inlet, the two sides of the air tight chamber are provided with fresh air machines respectively, a humidifier is arranged inside the air tight chamber, and an air pre-processing device is arranged on the part, outside the air inlet, in the house. The air pre-processing device structurally comprises a filtering chamber arranged on the part, outside the air inlet, of the house, and air inlets in the two sides of the filtering chamber are provided with filtering nets respectively.

The invention discloses a bacterial culture and storage device applied to a high-humidity environment, and belongs to the technical field of bacterial culture.According to the scheme, in the bacterial culture process, a humidity monitoring mechanism monitors the humidity change in a sterile culture box through a humidity sensor, and if the humidity is too high, the sterile culture box is started; the air-drying moisturizing mechanism pulls a moving plate and a sealing plug to be far away from an air outlet through an electric telescopic rod, so that hot air accumulated in a heat conduction ball is released, discharged upwards under pulling of the moving plate and flows through the inner side and the outer side of the sterile incubator, the humidity in the sterile incubator is reduced, and humidity balance is kept; the liquid cooling humidity supply mechanism sprays potassium nitrate powder through an electric spray head, the potassium nitrate powder is dissolved in an aqueous solution, the temperature is reduced, water vapor in the monitoring cabin is liquefied when cooled, air is promoted to keep moist, under pushing of an extrusion push block, the moist air is extruded oppositely to flow, the dryness of the sterile incubator is reduced, and a constant-humidity environment is created. Growth and reproduction of bacteria are promoted, and the culture effect is enhanced.

The invention discloses a supporting mold made through a laser carving method. A mold frame comprises a supporting frame mold and a fixing frame mold with a groove. The frame mold is rectangular and is made of polymethyl methacrylate (PMMA), and a bonding agent is trichloromethane. The fixing frame mold with the groove is made of glass. Compared with the prior art, the culture supporting frame canadapt to culture of artificial blood vessels different in diameter, and it is guaranteed that the blood vessels are cultured under the dynamic conditions. Kinetic study research, tumor cell migrationand medicine diffusion tests of the in-vitro blood vessels and other research can be conducted.

The invention discloses a multifunctional pathology incubator which comprises an incubator body, a culture frame, an incubator body handle, air leakage openings, a partition plate and a temperature controller. The partition plate is arranged in the incubator body, the incubator body is divided into two layers by the partition plate, the temperature controller is arranged at the upper end of the partition plate, a function lamp is arranged at the upper end of the incubator body, a limit groove is formed in the right side of the incubator body, sliding grooves are formed in the front end and therear end of the culture frame, sliding wheels are arranged at the front ends of the sliding grooves, the sliding grooves are rotatably connected with the sliding wheels, the incubator body is slidably connected with the sliding grooves in the culture frame through limit grooves, a baffle plate is arranged on the right side of the culture frame, limit holes are formed in the baffle plate, limit blocks are arranged on the right side of the incubator body, limit projections are arranged on the limit blocks, a culture frame handle is arranged on the right side of the baffle plate, the air leakageopenings are formed in the incubator body, different research objects are cultured through an upper layer and a lower layer in a classified manner, so that different conditions can be dealt, the culture frame 12 is slidably connected with the incubator body 1, taking is facilitated, and the incubator is convenient to carry by the aid of an integrated structure.

The invention discloses a suction method and an adding method of a biological tissue culture solution and relates to the technical field of biological tissue culture. The suction method comprises the following steps: extracting a negative pressure from a culture solution suction head, moving down the culture liquid suction head according to a first set distance, detecting a first state negative pressure in the culture solution suction head, and comparing the first state negative pressure with a first set negative pressure; if the first state negative pressure is greater than or equal to the first set negative pressure, stopping moving down the culture solution suction head and stopping extracting the negative pressure from the culture solution suction head, moving the culture solution suction head to a level of the culture solution, and discharging the solution in the culture solution suction head; moving down the culture solution suction head to a position below the level of the culture solution according to a second set distance and extracting a set amount of the culture solution; and after the culture solution is sucked, moving the culture solution suction head upwards and out of a culture solution bottle, and thus automatically sucking the biological tissue culture solution. The adding method comprises steps of uncovering, solution adding and covering. The biological tissue culture solution can be automatically added and a requirement for culturing biological tissues in batches is met.

The invention discloses an edible mushroom factory fresh air system. The edible mushroom factory fresh air system comprises a house, a passage arranged in the middle of the house, mushroom growing rooms arranged on the two sides of the passage inside the house, air conditioner internal machines arranged at the upper ends of the mushroom growing rooms, and exhaust fans arranged at the lower ends of the mushroom growing rooms. An air tight chamber is arranged at the upper end of the passage, one side of the air tight chamber is provided with an air inlet, an efficient filtering net is arranged at the air inlet, and the two sides of the air tight chamber are provided with fresh air machines respectively. The edible mushroom factory fresh air system has the advantages that the air inside the air tight chamber is similar to the air in the mushroom growing rooms, the air cleanliness is improved, the frequency of washing the fresh air machine filtering net is reduced, a controller is used for controlling the fresh air machines and is arranged inside the air tight chamber, the edible mushroom factory fresh air system looks more attractive, the fresh air machines can provide fresh air for the mushroom growing rooms, normal cultivation and growth of edible mushrooms can be guaranteed, and the pollution to fungus spores from the natural environment is reduced.

The invention belongs to the technical field of shaking incubators, in particular to a three-color light multi-section adjustable system. The three-color light multi-section adjustable system comprises an incubator, a cabinet door which is mounted on the incubator and is rotatably connected through a rotating shaft, and an observation window which is formed in the surface of the cabinet door and is used for observing the internal state of the incubator, a control panel is installed on the surface of the incubator, an LED illumination plate is arranged in the incubator, and a plurality of LED lamps are arranged on the surface of the LED illumination plate; the problem of culture difference at different positions is solved; the intensity of light sources around the illumination plate is adjusted; the illumination intensity around and in the middle is the same; the three light sources are ensured to be selectable or combinable, actual culture is met, multiple sections can be independentlyor simultaneously arranged, the use is convenient, the illumination intensity is adjustable, different culture requirements are met, a unique heat dissipation system is adopted, the temperature riseis low, the influence on culture is small, the actual problem can be effectively solved through the above points, and the actual requirements of users are met.

The invention discloses a simple and efficient unicellular algae separation and purification method, which comprises the following steps: accurately measuring the unicellular algae cell density of an algae solution to be separated and purified by using a blood cell counting plate under an optical microscope, diluting the algae solution to the unicellular algae cell density of 20-30 cells/mL by using sterilized sand filtration seawater, sucking 1-3 drops of diluted algae liquid according to the quantity of miscellaneous algae cells and harmful organisms in the algae liquid to be separated and purified, dripping the diluted algae liquid into a glass test tube filled with a unicellular algae culture solution, culturing for 10-15 days in a place with sufficient illumination and without direct sunlight, and carrying out microscopic examination and screening to obtain purified unicellular algae without miscellaneous algae cells and harmful organisms. The method disclosed by the invention is simple to operate, extremely low in requirement on professional degree of operators, wide in application place, suitable for laboratories with good conditions and farms with simple conditions, and capable of ensuring pure culture and long-term storage of the unicellular algae, and the success rate of separation and purification reaches 100%.

The invention belongs to the technical field of flora culturing, and discloses a preparation system of nematode No.1 battlefront flora. The system comprises a culturing module which is a device used for culturing nematode No.1 battlefront bacteria, a storage module which is used for the storage of the nematode No.1 battlefront bacteria and connected with the culturing module, a water injection module used for adding water into the storage module, a supplying module used for adding nutrient solution to the storage module and controlling the oxygen dissolving concentration of the storage moduleand a drainage module used for performing regular outward water drainage, and a detection module used for detecting the culturing and storing process and an alarm module used for giving prompts and alarms, wherein the water injection module, the supplying module and the drainage module are connected with the storage module, the detection module and the alarm module are connected with the culturingmodule and the storage module. According to the system, an automation technology is applied to the culturing process of the flora, the nematode No.1 battlefront flora can be effectively cultured, theoperation is simple and convenient and the culturing efficiency is high.