Artificial blood vessel supporting mold made through laser carving method

A mold and blood vessel technology, applied in the field of supporting molds prepared by laser engraving, can solve the problems of low frame precision, large size, expensive printing equipment, etc., and achieve the effect of simple structure, strong practicability, and easy promotion

Inactive Publication Date: 2018-12-25
ENERGY RES INST OF SHANDONG ACAD OF SCI
View PDF4 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the compatibility support frame adopts 3D printing method, but the frame printed by this method has low precision, rough surface and large size. At the same time, the printing equipment is expensive and the technology is immature

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Artificial blood vessel supporting mold made through laser carving method
  • Artificial blood vessel supporting mold made through laser carving method
  • Artificial blood vessel supporting mold made through laser carving method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] A support frame mold suitable for artificial blood vessel culture of different diameters, comprising: a mold body, the mold body is a hollow cavity, the hollow cavity is composed of a bottom surface and a side surface, and the side surface is composed of a hole passing through the center point of the hollow cavity The cross section is divided into side I and side II, the side I is provided with at least one through hole, and the side II is respectively provided with at least two through holes, such as figure 1 shown.

[0043] The concrete method of adopting laser engraving method to prepare above-mentioned mold is as: adopting polymethyl methacrylate (PMMA) as mold material, adopting computer design software coredraw to design the long side dimension of mold is 1cm * 4cm, and short side dimension is

[0044] 1cm×2cm, using chloroform as the adhesive, with a thickness of 1mm. After the combination of the short sides is the width of the mold, there are 5 holes arranged s...

Embodiment 2

[0046] The mold of embodiment 1 is used for biomechanical detection of artificial blood vessels in vitro, specifically including:

[0047] The in vitro artificial blood vessels are inserted into the corresponding apertures in the mold of Example 1 in sequence, similar to figure 2 ②, then use a micrometer to measure its thickness, and then use a universal tensile tester (Instron-3343) to detect its mechanical properties and calculate the elastic modulus.

Embodiment 3

[0049] The mold of Example 1 is applied to the preparation of a hydrogel chip, specifically including:

[0050] 1) Select the mold prepared in Example 1 that meets the following requirements: the aperture diameter is 400 μm, and the two long sides of the mold are symmetrically provided with 8 through holes, and one end of the two short sides is provided with 1 through hole, and one end is provided with There are 2 two through holes (the above through holes are all located on the same level), with glass as the bottom surface.

[0051] 2) Prepare an aqueous gelatin solution with a mass volume ratio of 12.5%, add transglutaminase (TG enzyme) with a mass volume ratio of 10%, mix the gelatin solution and TG enzyme at room temperature, and then pour the gelatin solution for the first time. Stop pouring when the surface is flush with the lower edge of the through hole, place the glass capillary in the mold, assemble the required dendritic channel structure, and check the relative pos...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
pore sizeaaaaaaaaaa
pore sizeaaaaaaaaaa
Login to view more

Abstract

The invention discloses a supporting mold made through a laser carving method. A mold frame comprises a supporting frame mold and a fixing frame mold with a groove. The frame mold is rectangular and is made of polymethyl methacrylate (PMMA), and a bonding agent is trichloromethane. The fixing frame mold with the groove is made of glass. Compared with the prior art, the culture supporting frame canadapt to culture of artificial blood vessels different in diameter, and it is guaranteed that the blood vessels are cultured under the dynamic conditions. Kinetic study research, tumor cell migrationand medicine diffusion tests of the in-vitro blood vessels and other research can be conducted.

Description

technical field [0001] The invention belongs to the fields of micromachining technology, biological tissue engineering and biomedicine, and in particular relates to a supporting mold prepared by laser engraving, and the mold can be used for performance characterization of artificial blood vessels in vitro and manufacture of vascularized artificial organs in vitro. Background technique [0002] Laser engraving technology uses laser as the processing medium and has a wide range of processing materials. It can be used not only for non-metallic materials such as plexiglass, plastic, glass, but also for common metal materials such as alumina. The processing process is simple, the precision is high, and the speed is fast. Computer modeling can be used to quickly obtain the mold of the required structure. [0003] Cardiovascular disease is a disease with increasing morbidity and mortality and a serious threat to human health. Transplantation using the own blood vessels of patient...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): A61F2/06
CPCA61F2/06A61F2240/004
Inventor 王雪莹赵培田寒梅王建梅王立秋
Owner ENERGY RES INST OF SHANDONG ACAD OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap