Suction method and adding method of biological tissue culture solution

A technology of biological tissue and culture fluid, applied in biochemical equipment and methods, tissue cell/virus culture device, bioreactor/fermenter combination, etc., can solve the problem of embryo culture that is difficult to meet the needs of automation, intelligence and batch production needs and other issues to achieve the effect of ensuring normal cultivation

Pending Publication Date: 2021-11-30
重庆市盛佰昱科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the technical problem of using artificial liquid addition in the existing human embryo culture, it is difficult to meet the needs of automation, intelligence, and batch embryo culture; the invention provides a method for absorbing biological tissue culture liquid, which can automatically add biological tissue culture liquid Aspirated into the culture solution tip to meet the needs of batch culture of biological tissue

Method used

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  • Suction method and adding method of biological tissue culture solution
  • Suction method and adding method of biological tissue culture solution
  • Suction method and adding method of biological tissue culture solution

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0087] to combine Figure 5 , based on the above-mentioned liquid adding device, the biological tissue culture fluid absorption method provided in this embodiment includes the following steps:

[0088] The culture solution tip is set on a plug-in rod, that is, the transverse movement component 600 drives the vertical movement component 700 to move laterally, and the longitudinal movement component 700 moves the liquid suction component 400 to the top of the corresponding culture solution tip socket, and then passes through the second A linear drive mechanism 403 drives the lower end of the insertion rod 401 to insert into the socket of the culture solution tip, so that the culture solution tip is sleeved on the lower end of the insertion rod 401, so that the inner cavity of the culture solution tip communicates with the suction channel;

[0089] According to the coordinates of the culture solution bottle where the culture solution is stored, the culture solution tip is moved t...

Embodiment 2

[0108] to combine Figure 6 , the present embodiment provides a biological tissue culture suction method, including the steps described in Example 1 and the cover oil suction step, the cover oil suction step is:

[0109] Set the cover oil suction head on a plug-in rod, that is, drive the vertical movement assembly 700 to move horizontally through the transverse movement assembly 600, and the vertical movement assembly 700 moves the liquid suction assembly 400 to the top of the corresponding cover oil suction head socket, and then pass through the second A linear drive mechanism 403 drives the lower end of the plugging rod 401 to insert into the socket of the cover oil suction head, so as to socket the cover oil suction head on the lower end of the insertion rod 401, so that the inner cavity of the cover oil suction head communicates with the suction channel;

[0110] According to the coordinates of the cover oil bottle where the cover oil is stored, the cover oil suction head ...

Embodiment 3

[0129] combine Figure 7 , this embodiment provides a method for adding liquid to biological tissue culture, including the steps described in Example 2 and the following steps:

[0130] Move the vacuum suction cup to the top of the culture dish to be added, and move the vacuum suction cup down to make the vacuum suction cup touch the dish cover, that is, the vertical movement assembly 700 is driven by the traverse assembly 600 to move laterally, and the vertical movement assembly 700 will The cover assembly 500 is moved to the top of the petri dish, and then the third linear drive mechanism 502 drives the suction cup 501 to move down and close to the dish cover of the petri dish;

[0131] Apply negative pressure to the vacuum suction cup, move the vacuum suction cup upward to remove the lid from the petri dish, and remove the vacuum suction cup from above the petri dish, specifically, through the third linear drive The retraction of the mechanism 502 moves the dish cover upwa...

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Abstract

The invention discloses a suction method and an adding method of a biological tissue culture solution and relates to the technical field of biological tissue culture. The suction method comprises the following steps: extracting a negative pressure from a culture solution suction head, moving down the culture liquid suction head according to a first set distance, detecting a first state negative pressure in the culture solution suction head, and comparing the first state negative pressure with a first set negative pressure; if the first state negative pressure is greater than or equal to the first set negative pressure, stopping moving down the culture solution suction head and stopping extracting the negative pressure from the culture solution suction head, moving the culture solution suction head to a level of the culture solution, and discharging the solution in the culture solution suction head; moving down the culture solution suction head to a position below the level of the culture solution according to a second set distance and extracting a set amount of the culture solution; and after the culture solution is sucked, moving the culture solution suction head upwards and out of a culture solution bottle, and thus automatically sucking the biological tissue culture solution. The adding method comprises steps of uncovering, solution adding and covering. The biological tissue culture solution can be automatically added and a requirement for culturing biological tissues in batches is met.

Description

technical field [0001] The invention relates to the technical field of biological tissue culture, in particular to a method for absorbing and adding liquid to a biological tissue culture liquid. Background technique [0002] In Vitro Fertilization (In Vitro Fertilization) refers to the technology in which mammalian sperm and eggs complete the fertilization process in an artificially controlled environment outside the body. It is abbreviated as IVF in English. The existing in vitro culture of human embryos is achieved by placing the culture dish containing the embryos in a time-lag incubator or a conventional carbon dioxide incubator, while ensuring the temperature, humidity and cleanliness of the culture environment. [0003] When the culture dish for culturing the embryos is placed in the incubator, it is necessary to add the culture solution and cover oil for the culture of the embryos to the culture dish. In the prior art, the culture medium and the cover oil are usually...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/00C12M1/34C12M1/36
CPCC12M29/14C12M41/00C12M23/38C12M21/08
Inventor 蓝湘源古玉琼
Owner 重庆市盛佰昱科技有限公司
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