Active dry saccharomyces boulardii feed additive and its preparation method and use

A technology for yeast feed and Boula yeast, applied in the directions of microorganism-based methods, biochemical equipment and methods, applications, etc., can solve the problems of low fermentation level, low number of viable bacteria in the product, low price, etc., and achieve low moisture content. , to ensure the effect of cultivation and fermentation effect

Active Publication Date: 2013-06-12
山东盛泰生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] At present, most of the feed additives widely used in the market are antibiotic drugs, which not only have high cost of use, but also have various negative effects. , not only reduces the quality of livestock and poultry products, but also forms a food chain, affects the immune effect of humans and animals, and lays hidden dangers for the control of epidemic diseases; the third is to destroy the micro-ecological balance in the intestinal tract of animals, creating conditions for the invasion of microorganisms outside the body, thus easily Cross-infection caused by pathogenic bacteria
[0003] In order to overcome the deficiencies in the application of antibiotic feed additives, research and development of prebiotic feed additives has been started at home and abroad, but the products currently developed are mostly compound prebiotics made by several microorganisms fermented individually or jointly. or a single inactive yeast protein
The former, on the one hand, because most of the microorganisms are bacterial probiotics, have natural defects to antibiotics, and today the use of antibiotics has not been completely banned, which greatly limits the application of this type of compound probiotics; Separate or joint fermentation not only requires large equipment investment, complex process and high cost, but also because of the different growth conditions of each microorganism, it is extremely difficult to control the fermentation process during joint fermentation, resulting in low fermentation level and low number of live bacteria in the product, etc. Shortcomings also affect the development and application of this type of probiotics
The latter is mostly developed into inactive yeast protein in the use of yeast and added to the feed. Not only the biological effect is limited, the value cannot be fully reflected, but also because the product is cheap and the economic benefit is not high
Although the research on active dry yeast has been done for a long time, they are all used as a part of compound prebiotics, and a single high-efficiency active dry yeast prebiotic has not been developed and applied in large quantities.
[0004] The research results show that Saccharomyces boulardii has multiple mechanisms of action on the prevention and treatment of pathogenic intestinal diseases, and its bacterial agent is currently mainly used in the treatment of human diarrhea, while its research as a feed additive is only in the basic experimental stage, and has not yet formed an industry change

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Under sterile conditions, the Saccharomyces boulardii strains were inoculated into the activation medium containing 2wt% glucose, 0.5wt% peptone, 0.5wt% yeast extract powder and 1.5wt% agar, at 30°C The activated seed liquid was obtained by activating and culturing in medium for 52 hours. The activated seed solution is inoculated into the primary seed tank filled with the primary medium, the primary medium contains corn soaking water with 4wt% amino nitrogen, 0.5wt% ammonium sulfate, glucose crystallization mother liquor with a glucose value of 7wt%, phosphoric acid Potassium dihydrogen 0.2% and magnesium sulfate 0.05%, the amount of corn soaking water is 1 / 2 of the primary seed tank, the pH value of the primary medium is 4.5, the initial glucose content is 8%, and the primary expansion cultivation During the process, the ventilation ratio was kept at 0.5:1, the dissolved oxygen was controlled to 20% by stirring, and the temperature was cultivated at 30°C for 20 hours t...

Embodiment 2

[0034] Under sterile conditions, the Saccharomyces boulardii strains were inoculated into the activation medium containing 4wt% glucose, 0.7wt% peptone, 0.7wt% yeast extract powder and 2.5wt% agar, at 32°C The activated seed liquid was obtained by activating and culturing for 42 hours. The activated seed solution is inoculated into the first-level seed tank filled with the first-level medium, and the first-level medium contains corn soaking water with 5wt% amino nitrogen, ammonium sulfate 0.7wt%, glucose crystallization mother liquor with a glucose value of 8wt%, phosphoric acid Potassium dihydrogen 0.5% and magnesium sulfate 0.07%, the amount of corn soaking water is 1 / 2 of the primary seed tank, the pH value of the primary medium is 5.5, the initial glucose content is 12%, and the primary expansion cultivation During the process, the ventilation ratio was kept at 0.8:1, the dissolved oxygen was controlled to 30% by stirring, and the temperature was cultivated at 32°C for 15 ...

Embodiment 3

[0038] Under sterile conditions, the Saccharomyces boulardii strains were inoculated into the activation medium containing 3wt% glucose, 0.6wt% peptone, 0.6wt% yeast extract powder and 2.0wt% agar, at 31°C The activated seed solution was obtained by activating in medium for 48 hours. Inoculate the activated seed solution into the first-level seed tank filled with the first-level medium. The first-level medium contains corn soaking water with 4.5wt% amino nitrogen, 0.6wt% ammonium sulfate, and glucose crystallization mother liquor with a glucose value of 7.5wt%. , potassium dihydrogen phosphate 0.4% and magnesium sulfate 0.06%, the amount of corn soaking water is 1 / 2 of the primary seed tank, the pH value of the primary medium is 5.0, and the initial glucose content is 10%. During the expansion process, the ventilation ratio was kept at 0.7:1, the dissolved oxygen was controlled to 25% by stirring, and the temperature was cultivated at 31°C for 18 hours to obtain a primary cult...

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Abstract

The invention discloses an active dry saccharomyces boulardii feed additive and its preparation method and use. The preparation method of the active dry saccharomyces boulardii feed additive comprises the following steps of carrying out activation cultivation, enlarging cultivation and fermentation cultivation of a saccharomyces boulardii strain to obtain fresh yeast, and carrying out low temperature drying of the fresh yeast to obtain the active dry saccharomyces boulardii feed additive having water content less than or equal to 6 wt% and the viable count equal to or greater than 40000 million per gram. An addition amount of the active dry saccharomyces boulardii feed additive in chicken feed is in a range of 100 to 300 grams per ton and an addition amount of the active dry saccharomycesboulardii feed additive in pig feed is in a range of 300 to 1000 grams per ton. The active dry saccharomyces boulardii feed additive has natural antibiotic resistance thus overcoming defects of composite probiotics and feed yeast in application. The active dry saccharomyces boulardii feed additive can adjust microecological balance of an intestinal tract, improve livestock and poultry body immunity and absorption and digestion of feed nutrients, promote livestock and poultry growth and reduce a breeding cost.

Description

technical field [0001] The invention relates to the technical field of animal feed additives, in particular to an active dry yeast boulardii feed additive. Background technique [0002] At present, most of the feed additives widely used in the market are antibiotic drugs, which not only have high cost of use, but also have various negative effects. , not only reduces the quality of livestock and poultry products, but also forms a food chain, affects the immune effect of humans and animals, and lays hidden dangers for the control of epidemic diseases; the third is to destroy the micro-ecological balance in the intestinal tract of animals, creating conditions for the invasion of microorganisms outside the body, thus easily Cause cross-infection of pathogenic bacteria. [0003] In order to overcome the deficiencies in the application of antibiotic feed additives, research and development of prebiotic feed additives has been started at home and abroad, but the products currentl...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23K1/16C12N1/16C12R1/645
Inventor 刘伟杰付刚丛伟国刘德林房贤坤刘雨李虎
Owner 山东盛泰生物科技有限公司
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