Preparation method of donkey-hide gelatin small molecular peptide

A technology of small molecule peptides and donkey-hide gelatin, which is applied in the field of preparation of donkey-hide gelatin small-molecule peptides, can solve problems such as indigestion and absorption, increasing the burden of gastrointestinal digestion, and difficulty in achieving drug efficacy due to poor digestion and absorption of donkey-hide gelatin.

Inactive Publication Date: 2016-06-22
蔡庭守
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most donkey-hide gelatin in the market is oral ointment. The high content of collagen is difficult to digest and absorb in the human body, especially for patients with weak spleen and stomach. Oral donkey-hide gelatin is less digested and absorbed so that it is difficult to achieve the drug effect, and it also increases the burden of gastrointestinal digestion.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A preparation method of donkey-hide gelatin small molecule peptide, comprising the following steps:

[0025] (1) Raw material pretreatment: Carry out vacuum drying to the donkey-hide gelatin raw material until the water content is 4.8%, then pulverized into donkey-hide gelatin fine powder with a particle diameter of 150 microns;

[0026] (2) Enzymolysis: add water 13 times the weight of donkey-hide gelatin fine powder to donkey-hide gelatin fine powder, heat to 95°C after mixing evenly, keep warm for 15 minutes, then cool down to 50°C, adjust pH to 8.5, add alkaline protease for the second step For one enzymolysis, the amount of alkaline protease used is 1% of the weight of donkey-hide gelatin fine powder; then keep the temperature at 50°C, adjust the pH to 7.3, add neutral protease to carry out the second enzymolysis, the use amount of neutral protease is 1% of the weight of donkey-hide gelatin fine powder; finally keep the temperature at 50°C, adjust the pH to 3.5, ad...

Embodiment 2

[0031] A preparation method of donkey-hide gelatin small molecule peptide, comprising the following steps:

[0032] (1) Raw material pretreatment: The donkey-hide gelatin raw material was vacuum-dried at 55° C. for 16 hours until the moisture content was 5%, and then crushed into donkey-hide gelatin fine powder with a particle size of 150 microns.

[0033] (2) Enzymolysis: add water 12 times the weight of donkey-hide gelatin fine powder to donkey-hide gelatin fine powder, heat to 96°C after mixing evenly, keep warm for 16 minutes, then cool down to 48°C, use 10% NaOH solution to adjust the pH to 8.6, add Bacillus subtilis alkaline protease for the first enzymolysis, the amount of alkaline protease used is 1% of the weight of donkey-hide gelatin fine powder, the activity of alkaline protease is 500000u / g, and the enzymatic hydrolysis temperature of alkaline protease is 48°C , the enzymolysis time of alkaline protease is 3 hours; then the temperature is raised to 50°C, the pH is...

Embodiment 3

[0038] A preparation method of donkey-hide gelatin small molecule peptide, comprising the following steps:

[0039] (1) Raw material pretreatment: The donkey-hide gelatin raw material was vacuum-dried at 50° C. for 17 hours until the water content was 5%, and then crushed into donkey-hide gelatin fine powder with a particle size of 200 microns.

[0040] (2) Enzymolysis: add water 10 times the weight of donkey-hide gelatin fine powder to donkey-hide gelatin fine powder, heat up to 98°C after mixing evenly, keep warm for 10 minutes, then cool down to 55°C, use 10% NaOH solution to adjust the pH to 9.0, adding Bacillus subtilis alkaline protease to carry out the first enzymolysis, the usage amount of alkaline protease is 1% of donkey-hide gelatin fine powder weight, the activity of alkaline protease is 500000u / g, the enzymolysis temperature of alkaline protease is 55 ℃ , the enzymolysis time of alkaline protease is 2.5 hours; then keep the temperature at 55°C, use 10% HCl solutio...

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Abstract

The invention relates to a preparation method of a donkey-hide gelatin small molecular peptide. Donkey-hide gelatin is adopted as a main raw material, and the donkey-hide gelatin small molecular peptide is prepared by adopting the technologies such as raw material pretreatment, enzymolysis, filtration, decolorization and drying, wherein an enzymolysis process is characterized in that enzymolysis is carried out for multiple times by adopting multiple proteases, and collagen in the donkey-hide gelatin is treated by enzymolysis to obtain a small molecular peptide easy to be digested and absorbed by a human body. The preparation method of the donkey-hide gelatin small molecular peptide has the beneficial effects that a compound biological enzyme enzymolysis technology is utilized, enzymolysis is carried out by sequentially adopting alkaline protease, neutral protease and pepsin for enzymolyzing macromolecular protein contained in the donkey-hide gelatin into the donkey-hide gelatin small molecular peptide, the molecular weight of the obtained donkey-hide gelatin small molecular peptide is mainly distributed within 100-1000D, and the obtained donkey-hide gelatin small molecular peptide is mostly a small molecular peptide such as dipeptide, tripeptide and tetrapeptide, belongs to a small molecular structure and is extremely easily absorbed and utilized by the human body.

Description

technical field [0001] The invention relates to a preparation method, in particular to a preparation method of donkey-hide gelatin small molecule peptide. Background technique [0002] Donkey-hide gelatin is hard and brittle, the cross-section is bright, and the fragments are brown and translucent when viewed against light. Donkey-hide gelatin contains a large amount of collagen, as high as more than 80%. It has the functions of nourishing blood, nourishing yin, moistening dryness, and stopping bleeding. It is a good blood tonic. Most donkey-hide gelatin in the market is oral ointment in paste form. The high content of collagen is difficult to digest and absorb in the human body, especially for patients with weak spleen and stomach. Oral donkey-hide gelatin is less digested and absorbed so that it is difficult to achieve the efficacy of the medicine, and it also increases the burden of gastrointestinal digestion. Contents of the invention [0003] In order to solve the ab...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/36C07K1/34C07K1/14
CPCC07K1/14C07K1/34C07K1/36C12P21/06
Inventor 蔡庭守
Owner 蔡庭守
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