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A kit for detecting intestinal barrier function

A kit and solvent technology, applied in the field of biological products, can solve the problems of large trauma, complicated operation, difficult to popularize, etc., and achieve the effects of high accuracy, simple and fast operation, and simple operation.

Active Publication Date: 2017-06-06
北京中生金域诊断技术股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method is cumbersome to operate, requires patients to take corresponding substances that are not easily absorbed, and requires corresponding testing instruments, so it is difficult to promote clinically.
Bacterial culture is commonly used in the diagnosis of bacterial translocation. Usually, mesenteric lymph nodes are taken under sterile conditions for bacterial culture. Bacteria are positive when cultured. However, this method is quite traumatic and difficult to carry out clinically.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Example 1 Preparation of intestinal barrier function detection kit

[0046] The present embodiment provides the preparation of the intestinal barrier function detection kit, and the preparation method is as follows:

[0047] 1) Preparation of reaction substrate:

[0048] The reaction substrate is preferably but not limited to be made of ABS engineering plastics, and is provided with blank control holes, diamine oxidase reaction holes, D-lactic acid reaction holes and bacterial endotoxin reaction holes, and each reaction hole is filled with lower The corresponding reaction pads are described; the size of blank control wells and reaction wells can be preferred

[0049] 2) Preparation of plasma separator:

[0050] The structure of the plasma separation membrane in the plasma separator may be conventional in the prior art, which is not particularly limited in the present invention. Whole blood is dropped on the plasma separation membrane to achieve the separation of s...

Embodiment 2

[0094] Example 2 Using method of intestinal barrier function detection kit

[0095] This embodiment provides the use method of the intestinal barrier function detection kit, specifically:

[0096] After taking a small amount of whole blood from the fingertip with a blood collection needle, it is directly added to the plasma separator of the device. After the whole blood is separated by the plasma separation membrane, the serum reaches each reaction hole of the device, and is in the diamine oxidase and D lactic acid reaction holes. Add one drop (about 10 μL) of diluent, and add one drop (about 10 μL) of chromogenic solution to the bacterial endotoxin reaction well. The reaction device was placed on the dry chemical detection instrument for 15 min. Through quantitative interpretation by dry chemical analyzer, the concentration of the analyte can be quantitatively obtained.

Embodiment 3

[0097] Example 3 Application of intestinal barrier function detection kit

[0098] This kit provides a detection kit for intestinal barrier function. It can detect blood samples of patients in clinic to check whether the intestinal barrier function of the patient is impaired, so as to assist the patient to reconstruct the intestinal barrier function.

[0099] The specific use of the kit is as follows:

[0100]1) When using, first take out the kit from 2°C to 8°C, equilibrate to room temperature and start using.

[0101] 2) Tear off the aluminum foil package and take out the reaction device.

[0102] 3) Use blood collection tubes to collect whole blood from fingertips and add it directly to the blood injection hole of each reaction well of the device; if the sample is serum or plasma, the plasma separation device needs to be torn off and added to each reaction well. 4 μL of sample is sufficient.

[0103] 4) Stand for a while until the blood has fully penetrated, and then rem...

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PUM

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Abstract

The invention provides a kit for detecting the barrier function of the intestinal tract. The kit comprises a reaction device, one or more colorimetric solutions and one or more dilution solutions; the reaction device comprises a blood plasma separator and a reaction substrate, and the reaction substrate includes a blank hole, a diamine oxidase reaction hole, a D-lactic acid reaction hole and a bacterial endotoxin reaction hole; a blood plasma separating film is arranged in each of the holes of the blood plasma separator; and a blank pad is arranged in the blank hole.

Description

technical field [0001] The invention relates to the technical field of biological products, in particular to a kit for detecting intestinal barrier function, and more particularly, to a kit for detecting D-lactic acid, diamine oxidase and bacterial endotoxin in blood. Background technique [0002] Intestinal barrier dysfunction is mainly manifested as impaired intestinal mucosal barrier, intestinal microecological disturbances and intestinal motility disorders. Intestinal barrier dysfunction is caused by various causes of intestinal mucosal damage, atrophy, increased intestinal permeability, and dysbiosis of intestinal flora, resulting in the translocation of bacteria and / or endotoxins, and can induce and / or aggravate systemic inflammatory responses. and multiple organ dysfunction. Intestinal barrier dysfunction has an important impact on the occurrence, development and prognosis of critical diseases. Intestinal barrier dysfunction is common in critically ill patients, but...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/52
Inventor 李维勤王加义桂春爽董婷婷
Owner 北京中生金域诊断技术股份有限公司
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