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Molecularly imprinted adsorbent for removing blood bilirubin and preparation method thereof

A technology of molecular imprinting and bilirubin, which is applied in the field of blood purification system, can solve the problems of large imprinted polymer particles, low adsorption rate of adsorbent, difficult elution, etc., and achieve the effect of good biocompatibility and high specific surface area

Active Publication Date: 2018-05-08
佛山市博新生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the imprinted polymer particles prepared by the traditional embedding molecular imprinting polymerization method are large and not uniform enough, and the template molecules are embedded too deeply or too tightly, and the elution is difficult; The quality is slow, and it is not easy to combine with the recognition site
Therefore, the adsorption rate of the adsorbent prepared by the traditional embedding molecular imprinting polymerization method is relatively low, and it is difficult to meet the performance required for clinical application.

Method used

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  • Molecularly imprinted adsorbent for removing blood bilirubin and preparation method thereof
  • Molecularly imprinted adsorbent for removing blood bilirubin and preparation method thereof
  • Molecularly imprinted adsorbent for removing blood bilirubin and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Example 1: Place 1ml of solid porous polyvinyl alcohol microspheres in a 50ml three-necked flask, add 30ml of 50% ethanol solution and 0.05g of cerium ammonium nitrate, start 15min under nitrogen protection, and then add 0.20g of monomer acrylic acid dropwise N,N-diethylaminoethyl ester was stirred and refluxed for 3h at a temperature of 60°C. After the reaction is completed, the reactant is taken out and filtered with suction, and the unreacted monomer and initiator are washed with acetone and distilled water.

[0017] Place the modified porous polyvinyl alcohol microspheres in a 50ml Erlenmeyer flask, soak them in saline for about 30 minutes, absorb the surface water, add 10ml of human plasma rich in bilirubin, and keep it at 37°C away from light Shake at a constant temperature for 3 hours, remove and filter, wash the plasma on the surface with 0.1 mol / L PBS solution to balance, then transfer the microspheres to an Erlenmeyer flask containing 10ml of 50% ethanol solution...

Embodiment 2

[0018] Example 2: Put 2ml of the settled porous chitosan microspheres in a 50ml three-necked flask, add 30ml of 50% ethanol solution and 0.02g of benzoyl peroxide, start 15min under nitrogen protection, and then add 0.40g of mono N,N-diethylaminoethyl acrylate, stirred and refluxed for 4h at a temperature of 50°C. After the reaction is completed, the reactant is taken out and filtered with suction, and the unreacted monomer and initiator are washed with acetone and distilled water.

[0019] Place the modified porous chitosan microspheres in a 50ml Erlenmeyer flask, soak in physiological saline for about 30 minutes, absorb the surface water, add 20ml of human plasma rich in bilirubin, and keep it at 37°C away from light Shake at a constant temperature for 2 hours, remove and filter, wash the plasma on the surface with 0.1 mol / L PBS solution to balance, then transfer the microspheres to an Erlenmeyer flask containing 20 ml of 50% ethanol solution, and add 0.20 g double Phenol A di...

Embodiment 3

[0020] Example 3: Put 5ml of the settled porous polystyrene divinylbenzene microspheres in a 100ml three-necked flask, add 60ml of 50% ethanol solution and 0.05g potassium persulfate, and start under nitrogen protection for 10 minutes, then add 1.0g dropwise The monomer N-(3-dimethylaminopropyl)methacrylamide was stirred and refluxed for 3h at a temperature of 60°C. After the reaction is completed, the reactant is taken out and filtered with suction, and the unreacted monomer and initiator are washed with acetone and distilled water.

[0021] Measure 2ml of modified porous styrene divinylbenzene microspheres and place them in a 50ml Erlenmeyer flask, soak in saline for about 30 minutes, absorb the surface water, add 20ml of human plasma rich in bilirubin, At temperature, shake at a constant temperature for 2 hours in the dark, remove and filter, wash the plasma on the surface with 0.1M PBS solution to balance, then transfer the microspheres to an Erlenmeyer flask containing 20ml ...

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Abstract

The invention provides a molecular imprinting adsorbent for eliminating blood bilirubin and a preparation method thereof. Imprinting sites are distributed on or close to the surface of a porous adsorption vector. A branched chain with the adsorption capacity for bilirubin is grafted to the surface of the porous adsorption vector. A bilirubin protein combination object is used as an imprinting template molecule, the porous adsorption vector with the proper pore diameter, the large specific surface area and the good biological compatibility is selected, and the molecular imprinting adsorbent for eliminating blood bilirubin is obtained by grafting the side chain with alkali groups and imprinting, crosslinking and eluting template molecules, the adsorbent can recognize bilirubin objects and perform specific adsorption similar to combination of an antibody and an antigen. The adsorption performance is good, and the good biological compatibility is achieved. The prepared adsorbent can be used for blood perfusion or plasma adsorption for treating hyperbilirubinemia.

Description

Technical field [0001] The invention relates to the technical field of blood purification systems, in particular to a molecularly imprinted adsorbent for removing blood bilirubin and a preparation method thereof. Background technique [0002] Bilirubin is a product of heme metabolism and degradation. It is an endogenous toxin. Under normal physiological conditions, serum protein binds to bilirubin to help bilirubin transfer to the liver and be excreted. When the liver function is abnormal, the metabolism of bilirubin is impaired, causing it to accumulate in the blood. Excessive concentrations of bilirubin in the blood can cause obstructive jaundice and even acute renal failure. The normal serum total bilirubin concentration is when the serum bilirubin concentration is 1.7~17.1μmol / L (0.1~1mg / dL). High levels of bilirubin can interfere with cell metabolism, stimulate skin nerves, and inhibit cardiac conduction system. The formation of embolism in the tubules causes toxic effects ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01J20/26B01J20/30C08F261/04C08F251/00C08F257/02C08F251/02C08F265/04C08J9/26A61M1/36
CPCA61M1/3679A61M2202/0415B01J20/268C08F251/00C08F251/02C08F257/02C08F261/04C08F265/04C08J9/26C08J2351/00C08J2351/02A61M2202/0021
Inventor 姜建明梁达星
Owner 佛山市博新生物科技有限公司
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